EFFECTS OF ISCHEMIA-REPERFUSION ON RAT RENAL TISSUE ANTIOXIDANT SYSTEMS AND LIPID PEROXIDATION

  • B Rasoulian Razi Herbal Medicines Research Center and Department of Physiology, Lorestan University (Medical sciences), Khorramabad, Iran. AND Department of Physiology, School of Medicine, Lorestan University (Medical Sciences), Khorramabad, Iran.
  • M Jafari Trauma Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. AND Department of Biochemistry, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
  • A Noroozzadeh Department of Physiology and Biophysics, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
  • H Mehrani Department of Biochemistry, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
  • H Wahhab-Aghai Trauma Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. AND Department of Physiology and Biophysics, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
  • S.M.H Hashemi-Madani Research Center for Molecular Biology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
  • E Ghani Department of Physiology and Biophysics, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
  • M Esmaili Department of Physiology, School of Medicine, Yazd University of Medical Sciences, Yazd, Iran.
  • A Asgari Department of Physiology and Biophysics, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
  • A Khoshbaten Department of Physiology and Biophysics, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran. AND Research Center for Chemical Injuries, School of Medicine, Baqiyatallah University Medical Sciences, Tehran, Iran.
Keywords: Kidney, ischemia, reperfusion, antioxidant, malondialdehyde

Abstract

Renal ischemia-reperfusion (IR) injury is a common problem in kidney transplantation. There is increasing evidence about the role of the reactive oxygen species (ROS) in these injuries and endogenous antioxidants seem to have an important role in decreasing the renal tissue injury. The aim of this study was to investigate the effects of kidney IR on renal antioxidant enzymes activity such as catalase and superoxide dismutase (SOD) and renal glutathione (GSH) levels. The level of malondialdehyde (MDA), an end product of lipid peroxidation, also was quantified. Male Wister rats (200-280 g) were anesthetized and after right nephrectomy, the left renal artery was clamped for 40 min. After 24 h reperfusion, serum and kidney tissue samples were obtained for metabolites assay. Results showed that renal IR increased serum creatinine and urea level [90.59 ± 12.93 vs. 44.06 ± 4.07 µmol/L (P < 0.01), and 40.22 ± 10.27 vs. 20.8 ± 1.8 mmol/L, (P < 0.05), respectively]. The renal catalase activity was decreased (33.13 ± 2.02 vs. 43.78 ± 2.38 units/mg protein, P < 0.01) but SOD activity was increased (57.59 ± 4.64 vs. 42.84 ± 1.85 units/mg protein, P < 0.05). The GSH level also was decreased (24.67 ± 2.31 vs. 37.44 ± 3.09 nmol/mg protein P < 0.01). MDA level after IR was not significantly different from control group (2.05 ± 0.27 vs. 1.84 ± 0.2 nmol/mg protein, P = 0.55). Our data indicated that despite decrease in renal GSH level and catalase activity following IR, SOD activity was increased.
How to Cite
1.
Rasoulian B, Jafari M, Noroozzadeh A, Mehrani H, Wahhab-Aghai H, Hashemi-Madani S, Ghani E, Esmaili M, Asgari A, Khoshbaten A. EFFECTS OF ISCHEMIA-REPERFUSION ON RAT RENAL TISSUE ANTIOXIDANT SYSTEMS AND LIPID PEROXIDATION. Acta Med Iran. 46(5):353-60.
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