Ecology of Anopheles stephensi in a Malarious Area, Southeast of Iran

  • Ahmad Mehravaran Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Hassan Vatandoost Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Mohammad Ali Oshaghi Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Mohammad Reza Abai Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Hamideh Edalat Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. AND Kerman Province Health Center, Kerman University of Medical Sciences, Kerman, Iran.
  • Ezatoddin Javadian Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Minoo Mashayekhi Pasteur Institute of Iran, Tehran, Iran.
  • Norair Piazak Pasteur Institute of Iran, Tehran, Iran.
  • Ahmad Ali Hanafi-Bojd Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Keywords: Anopheles stephensi, Ecology, Iran, Malaria

Abstract

District of Jiroft is situated in south-east of Iran which is one of the malarious regions. Anopheles stephensi is considered as one of the main malaria vector in this region. Ecology of this species was studied in the area to understand its vector behavior for implementation of effective vector control measures. Different methods like total catch, pit shelter, night bite collection on human and animal, larval dipping methods were used for species identification, seasonal activity, anthropophilic index and egg morphological characteristics. Anthropophilicity index was assessed by ELISA test. Activity of Anopheles species started at the beginning of April, and its peak occurs in late spring. The larvae were found in the river bed with pools, stagnant streams, slow foothill streams, temporary pools, and slowly moving water with and without vegetation, drainage containers of air conditioner and palm irrigation canals. From different methods of adult collection, it was found that spray sheet collection is the appropriate method. ELISA testing of 144 blood meals of females revealed the anthropophilicity of 11.8% indicating host preference on animal, mainly cow. Ridge length and their number on the egg floats confirmed Anopheles stephensi mysorensis form. This study showed that Anopheles stephensi is the main vector of malaria in the region, although some other species may play a role. Our findings could provide a valuable clue for epidemiology and control of malaria in the southeast of Iran.

References

Raeisi A, Shahbazi A, Ranjbar M, Shoghli A, Vatandoost H, Faraji L. National Strategy Plan for Malaria Control (I.R.Iran, 2004-2008). Ministry of Health and Medical Education, 2004.

Manouchehri AV, Zaim M, Emadi AM. A review of malaria in Iran, 1957-1990. J Am Mosq Control Assoc 1992;8(4):381-5.

Zahirnia AH, Vatandoost H, Nateghpour M, Djavadian E. Insecticide resistance/susceptibility monitoring in Anopheles pulcherrimus (Diptera: Culicidae) in Ghasreghand district, Sistan and Baluchistan province, Iran. Iran J Publ Health 2002;31(1-2):11-4.

Vatandoost H. Irritability level of Anopheles sephensi inIran. Iran J Publ Health 2001;30(1-4):27-30.

Zahirnia AH, Taherkhani H, Vatandoost H. Observation of malaria sporozoite in Anopheles culicifacies (Diptera: Culicidae) in Ghasreghand district, Sistan and Baluchistan province. Hakim Res J 2001;4(2):149-53.

Enayati AA, Vatandoost H, Ladonni H, Townson H, Hemingway J. Molecular evidence for a kdr-like pyrethroid resistance mechanism in the malaria vector mosquito Anopheles stephensi. Med Vet Entomol 2003;17(2):138-44.

Naddaf SR, Oshaghi MA, Vatandoost H, Assmar M. Molecular characterization of Anopheles fluviatilis species complex in the Islamic Republic of Iran. East Mediterr Health J 2003;9(3):257-65.

Vatandoost H, Borhani N. Susceptibility and Irritability levels of main malaria vectors to synthetic pyrethroids in the endemic areas of Iran. Acta Med Iran 2004;42(4):240-7.

Vatandoost H, Moinvaziri VM. Larvicidal activity of neem tree extract (Neemarin) against mosquito larvae in the Islamic Republic of Iran. East Mediterr Health J 2004;10(4):573-8.

Vatandoost H, Shahi H, Abai MR, Hanafi-Bojd AA, Oshaghi MA, Zamani G. Larval habitats of main malaria vectors in Hormozgan province and their susceptibility to different larvicides. Southeast Asian J Trop Med Public Health 2004;35 Suppl 2:22-5.

Vatandoost H, Mashayekhi M, Abaie MR, Aflatoonian MR, Hanafi-Bojd AA, Sharifi I. Monitoring of insecticides resistance in main malaria vectors in a malarious area of Kahnooj district, Kerman province, southeastern Iran. J Vector Borne Dis 2005;42(3):100-8.

Vatandoost H, Hanafi-Bojd AA. Current resistant status of Anopheles stephensi Liston to different larvicides in Hormozgan province, southeastern Iran. Pakistan J Biol Sci 2005;8(11):1568-70.

Hanafi-Bojd AA, Vatandoost H, Jafari R. Susceptibility status of Anopheles dthali and An. fluviatilis to commonly used larvicides in an endemic focus of malaria, southern Iran. J Vector Borne Dis 2006;43(1):34-8.

Oshaghi MA, Chavshin AR, Vatandoost H, Yaaghoobi F, Mohtarami F, Noorjah N. Effects of post-ingestion and physical conditions on PCR amplification of host blood meal DNA in mosquitoes. Exp Parasitol 2006;112(4):232-6.

Oshaghi MA, Chavshin AR, Vatandoost H. Analysis of mosquito bloodmeals using RFLP markers. Exp Parasitol 2006;114(4):259-64.

Oshaghi MA, Yaghoobi F, Vatandoost H, Abai MR, Akbarzadeh K. Anopheles stephensi biological forms, geographical distribution, and malaria transmission in malarious regions in Iran. Pakistan J Biol Sci 2006;9(2):294-8.

Vatandoost H, Gholizadeh S, Abai MR, Djavadian E. Laboratory efficacy of protection rate of torn nets treated with pyrethroids, cyfluthrin, deltamethrin and permethrin against Anopheles stephensi (Diptera: Culicidae). J Biol Sci 2006;6(2):331-6.

Vatandoost H, Oshaghi MA, Abaie MR, Shahi M, Yaaghoobi F, Baghaii M, et al. Bionomics of Anopheles stephensi Liston in the malarious area of Hormozgan province, southern Iran, 2002. Acta Trop 2006;97(2):196-203.

Davari B, Vatandoost H, Oshaghi MA, Ladonni H, Enayati AA, Shaeghi M, Basseri HR, Rassi Y, Hanafi-Bojd AA. Selection of Anopheles stephensi with DDT and dieldrin and cross-resistance spectrum to pyrethroids and fipronil. Pestic Biochem Physiol 2007;89(2):97-103.

Abai MR, Mehravaran A, Vatandoost H, Oshaghi MA, Javadian E, Mashayekhi M, Mosleminia A, Piyazak N, Edallat H, Mohtarami F, Jabbari H, Rafi F. Comparative performance of imagicides on Anopheles stephensi, main malaria vector in a malarious area, southern Iran. J Vector Borne Dis 2008;45(4):307-12.

Vatandoost H, Ramin E, Rassi Y, Abai MR. Stability and wash resistance of local made mosquito bednets and detergents treated with pyrethroids against Anopheles stephensi. Iran J Arthropod-Borne Dis 2009;3(1):19‐28.

Salari Lak Sh, Vatandoost H, Entezarmahdi MR, Ashraf H, Abai MR, Nazari M. Monitoring of insecticide resistance in Anopheles sacharovi (Favre, 1903) in borderline of Iran, Armenia, Naxcivan and Turkey, 2001. Iran J Publ Health 2002;31(3‐4):96‐9.

Sedaghat MM, Linton Y-M, Nicolescu G, Smith L, Koliopoulos G, Zounos AK, Oshaghi MA, Vatandoost H, Harbach RE. Morphological and molecular characterization of Anopheles (Anopheles) sacharovi Favre, a primary vector of malaria in the Middle East. Syst Entomol 2003b;28:241-56.

Sedaghat MM, Linton YM, Oshaghi MA, Vatandoost H, Harbach RE. The Anopheles maculipennis complex (Diptera: Culicidae) in Iran: molecular characterization and recognition of a new species. Bull Entomol Res 2003;93(6):527-35.

Sedaghat MM, Harbach RE. An annotated checklist of the Anopheles mosquitoes (Diptera: Culicidae) in Iran. J Vector Ecol 2005;30(2):272-6.

Doosti S, Azari-Hamidian S, Vatandoost H, Oshaghi MA, Hosseini M. Taxonomic differentiation of Anopheles sacharovi and An.maculipennis S.l. (Diptera: Culicidae) larvae by seta 2 (antepalmate hair). Acta Med Iran 2006;44(1):21-7.

Doosti S, Vatandoost H, Oshaghi MA, Hosseini M, Sedaghat MM. Applying morphometric variation of seta 2 (antepalmate hair) among the larvae of the members of the Maculipennis subgroup (Diptera: Culicidae) in Iran. Iran J Arthropod-Borne Dis 2007;1(1):28-37.

Vatandoost H, Zahir Nia AH. Responsiveness of Anopheles maculipennis to different imagicides during resurgent malaria. Asia Pac J Trop Med 2010;3(5):360-3.

Krishnan KS. Anophele stephensi Liston 1901. In: Vectors of Malaria in India. 2nd ed. Delhi: National Society of India for Malaria and other Mosquito-born Disease; 1961. p. 38-57.

Manouchehri AV, Javadian E, Eshighy N, Motabar M. Ecology of Anopheles stephensi Liston in southern Iran. Trop Geogr Med 1976;28(3):228-32.

Davidson G, Jackson CE. DDT-resistance in Anopheles stephensi. Bull World Health Organ 1961;25(2):209-17.

Subbarao SK, Vasantha K, Adak T, Sharma VP, Curtis CF. Egg-float ridge number in Anopheles stephensi: ecological variation and genetic analysis. Med Vet Entomol 1987;1(3):265-71.

World Health Organization Practical entomology in malaria eradication. Part І. Field and laboratory techniques. 1963. WHO/PA/62.63: 8-11.

World Health Organization (WHO). Entomological Field Techniques for Malaria Control. Part II: Tutor's Guide Geneva: Nonserial Publication; 1992. p. 55.

Edrissian GH, Manouchehri AV, Hafizi A. Application of an enzyme-linked immunosorbent assay (ELISA) for determination of human blood index in anopheline mosquitoes collected in Iran. J Am Mosq Control Assoc 1985;13:349-52.

Sweet WC, Rao BA. Races of Anopheles stephensi Liston, 1901. Indian Med Gazette 1937;72:665-74.

Garcia LS. Diagnostic Medical Parasitology. 4th ed. Washington DC: American Society for Microbiology; 2001; p. 1092.

Rowland M, Mohammed N, Rehman H, Hewitt S, Mendis C, Ahmad M, Kamal M, Wirtz R. Anopheline vectors and malaria transmission in eastern Afghanistan. Trans R Soc Trop Med Hyg 2002;96(6):620-6.

Zahar AR. Vector bionomics in the epidemiology and control of malaria. Part II: The WHO European and the WHO Mediterranean region. Vol II. Applied field studies. Geneva: World Health Organization; 1990.

How to Cite
1.
Mehravaran A, Vatandoost H, Oshaghi MA, Abai MR, Edalat H, Javadian E, Mashayekhi M, Piazak N, Hanafi-Bojd AA. Ecology of Anopheles stephensi in a Malarious Area, Southeast of Iran. Acta Med Iran. 50(1):61-65.
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