Bacterial Analysis of Peri-implantitis and Chronic Periodontitis in Iranian Subjects
AbstractChronic periodontitis (CP) and peri-implantitis (PI) are multifactorial diseases of tooth and implant supporting apparatus. Bacterial invasion and consequent host immune response seem to play a role in relevant pathogenesis. The structural differences between tooth and implant pose preferential biofilm colonization. This study was aimed to compare the prevalence of bacteria in CP and PI. Clinical and radiographic examination performed over 69 individuals referred to Shahid Beheshti Dental School (Tehran, Iran) and four groups categorized: CP (n=22), HP (n=21), PI (n=13) and HI (n=13). The mean age was 45.6 years, 55% of participants were female and 45% were male. Bacterial samples were collected by paper point method and transferred to Institute of Odontology, University of Gothenburg (Gothenburg, Sweden) for checkerboard DNA-DNA hybridization. Kruskal-Wallis and Mann-Whitney U tests were used to compare distribution of bacteria in four groups. Significant differences were observed for T. forsythia, P. intermedia, C. rectus, P. endodontic, P. gingivalis, T. denticola and P. tannerae (P
Berglundh T, Zitzmann NU, Donati M. Are periimplantitis lesions different from periodontitis lesions? J Clin Periodontol 2011;38 Suppl 11:188-202.
Lang NP, Berglundh T. Working Group 4 of Seventh European Workshop on Periodontology. Periimplant diseases: where are we now? Consensus of the Seventh European Workshop on Periodontology. J Clin Periodontol2011;38 Suppl 11:178-81.
Kurien T, Deo V, Bhati A. The pouch and tunnel technique for the management of adjacent gingival recession defects: surgical correction and one-year follow-up. J Contemp Dent Pract 2010;11(5):041-8.
Heitz-Mayfield LJ, Lang NP. Comparative biology of chronic and aggressive periodontitis vs. peri-implantitis. Periodontol 2000 2010;53:167-81.
Jones DJ, Munro CL, Grap MJ. Natural history of dental plaque accumulation in mechanically ventilated adults: a descriptive correlational study. Intensive Crit Care Nurs 2011;27(6):299-304.
Meijndert L, van der Reijden WA, Raghoebar GM, Meijer HJ, Vissink A. Microbiota around teeth and dental implants in periodontally healthy, partially edentulous patients: is pre-implant microbiological testing relevant? Eur J Oral Sci 2010;118(4):357-63.
Standar K, Kreikemeyer B, Redanz S, Münter WL, Laue M, Podbielski A. Setup of an in vitro test system for basic studies on biofilm behavior of mixed-species cultures with dental and periodontal pathogens. PLoS One 2010;1;5(10). pii: e13135.
Cortelli JR, Fernandes CB, Costa FO, Cortelli SC, Kajiya M, Howell SC, Kawai T. Detection of periodontal pathogens in newborns and children with mixed dentition. Eur J Clin Microbiol Infect Dis 2012;31(6):1041-50.
Toyofuku T, Inoue Y, Kurihara N, Kudo T, Jibiki M, Sugano N, Umeda M, Izumi Y. Differential detection rate of periodontopathic bacteria in atherosclerosis. Surg Today 2011;41(10):1395-400.
Heuer W, Elter C, Demling A, Neumann A, Suerbaum S, Hannig M, Heidenblut T, Bach FW, Stiesch-Scholz M. Analysis of early biofilm formation on oral implants in man. J Oral Rehabil 2007;34(5):377-82.
Suzuki N, Yoshida A, Nakano Y. Quantitative analysis of multi-species oral biofilms by TaqMan Real-Time PCR. Clin Med Res 2005;3(3):176-85.
Ohnishi M. Quantitative analysis of periodontal pathogens in aggressive periodontitis patients in a Japanese population. Kokubyo Gakkai Zasshi 2006;73(1):70-8.
Pye AD, Lockhart DE, Dawson MP, Murray CA, Smith AJ. A review of dental implants and infection. J Hosp Infect 2009;72(2):104-10.
Sato J, Gomi K, Makino T, Kawasaki F, Yashima A, Ozawa T, Maeda N, Arai T. The evaluation of bacterial flora in progress of peri-implant disease. Aust Dent J 2011;56(2):201-6.
de Leitão JA, De Lorenzo JL, Avila-Campos MJ, Sendyk WR. Analysis of the presence of pathogens which predict the risk of disease at peri-implant sites through polymerase chain reaction (PCR). Braz Oral Res 2005;19(1):52-7.
Dahlén G, Leonhardt A. A new checkerboard panel for testing bacterial markers in periodontal disease. Oral Microbiol Immunol 2006;21(1):6-11.
1999 International workshop for a classification of periodontal diseases and conditions. Papers. Oak Brook, Illinois, October 30-November 2, 1999. Ann Periodontol 1999;4(1):i, 1-112.
Berglundh T, Persson L, Klinge B. A systematic review of the incidence of biological and technical complications in implant dentistry reported in prospective longitudinal studies of at least 5 years. J Clin Periodontol 2002;29 Suppl 3:197-212; discussion 232-3.
Ferreira SD, Silva GL, Cortelli JR, Costa JE, Costa FO. Prevalence and risk variables for peri-implant disease in Brazilian subjects. J Clin Periodontol 2006;33(12):929-35.
Roos-Jansåker AM, Lindahl C, Renvert H, Renvert S. Nine- to fourteen-year follow-up of implant treatment. Part II: presence of peri-implant lesions. J Clin Periodontol 2006;33(4):290-5.
Jung RE, Pjetursson BE, Glauser R, Zembic A, Zwahlen M, Lang NP. A systematic review of the 5-year survival and complication rates of implant-supported single crowns. Clin Oral Implants Res 2008;19(2):119-30.
López-Cerero L. Dental implant-related infections. Enferm Infecc Microbiol Clin 2008;26(9):589-92.
Fransson C, Wennström J, Berglundh T. Clinical characteristics at implants with a history of progressive bone loss. Clin Oral Implants Res 2008;19(2):142-7.
Zitzmann NU, Berglundh T. Definition and prevalence of peri-implant diseases. J Clin Periodontol 2008;35(8 Suppl):286-91.
Charalampakis G, Rabe P, Leonhardt A, Dahlén G. A follow-up study of peri-implantitis cases after treatment. J Clin Periodontol 2011;38(9):864-71.
Misch CE, Perel ML, Wang HL, Sammartino G, Galindo- Moreno P, Trisi P, Steigmann M, Rebaudi A, Palti A, Pikos MA, Schwartz-Arad D, Choukroun J, Gutierrez- Perez JL, Marenzi G, Valavanis DK. Implant success, survival, and failure: the international congress of oral implantologists (ICOI) pisa consensus conference. Implant Dent 2008;17(1):5-15.
Kadkhodazadeh M, Amid R. Implant success index (ISI): a new scoring system to evaluate the clinical outcomes of dental implants. J Clin Periodontol 2012; 39(Suppl): 209.
Charalampakis G, Leonhardt A, Rabe P, Dahlén G. Clinical and microbiological characteristics of periimplantitis cases: a retrospective multicentre study. Clin Oral Implants Res 2011.
Amoroso PF, Adams RJ, Waters MG, Williams DW. Titanium surface modification and its effect on the adherence of Porphyromonas gingivalis: an in vitro study. Clin Oral Implants Res 2006;17(6):633-7.
Leonhardt A, Olsson J, Dahlén G. Bacterial colonization on titanium, hydroxyapatite, and amalgam surfaces in vivo. J Dent Res 1995;74(9):1607-12.
Dowsett SA, Kowolik MJ, Archila LA, Eckert GJ, LeBlanc DJ. Subgingival microbiota of indigenous Indians of Central America. J Clin Periodontol 2002;29(2):159-67.
Natto S, Baljoon M, Dahlén G, Bergström J. Tobacco smoking and periodontal microflora in a Saudi Arabian population. J Clin Periodontol 2005;32(6):549-55.
Boutaga K, Savelkoul PH, Winkel EG, van Winkelhoff AJ. Comparison of subgingival bacterial sampling with oral lavage for detection and quantification of periodontal pathogens by real-time polymerase chain reaction. J Periodontol 2007;78(1):79-86.
Ata-Ali J, Candel-Marti ME, Flichy-Fernández AJ, Peñarrocha-Oltra D, Balaguer-Martinez JF, Peñarrocha Diago M. Peri-implantitis: Associated microbiota and treatment. Med Oral Patol Oral Cir Bucal 2011;16(7):e937-43.
do Nascimento C, Monesi N, Ito IY, Issa JP, de Albuquerque Junior RF. Bacterial diversity of periodontal and implant-related sites detected by the DNA Checkerboard method. Eur J Clin Microbiol Infect Dis 2011;30(12):1607-13.
Mombelli A, Décaillet F. The characteristics of biofilms in peri-implant disease. J Clin Periodontol 2011;38 Suppl 11:203-13.