Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 64 7 2026 07 12 428 434 EN Fatemeh Sadabadi Kidney Transplantation Complications Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.AND Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Maryam Saberi-Karimian Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Hamideh Ghazizadeh Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Mohsen Moohebati Vascular & Endovascular Surgery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. AND Department of Cardiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Habibollah Esmaily Department of Biostatistics & Epidemiology, Faculty of Health, Management & Social Determinants of Health Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Mohammad Sadegh Khorrami Mohebbseraj Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Sousan Darroudi Vascular & Endovascular Surgery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Zahra Asadi Student Research Committee, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Alireza Pasdar Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Amirhossein Sahebkar Biotechnology Research Center, Pharmaceutical Technology Institute, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. ASND Centre for Research Impact & Outcome, Chitkara College of Pharmacy, Chitkara University, Rajpura, Punjab 140401, India. Alireza Heidari-Bakavoli Vascular & Endovascular Surgery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. AND Department of Cardiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran . Sara Samadi Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran . Mahmoud Reza Azarpazhooh Department of Clinical Neurological Sciences, Western University, London, ON, Canada. Gordon A. Ferns Brighton and Sussex Medical School, Division of Medical Education, Brighton, UK. Amir Avan Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. AND Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Majid Ghayour-Mobarhan Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. AND International UNESCO Center for Health-Related Basic Sciences and Human Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran. AND Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. 2024 07 07 2025 04 02 Coronary heart disease (CHD) is a leading cause of death, highlighting the importance of risk stratification and prognostic biomarkers in CHD. There is a growing body of evidence supporting the potential value of heat shock proteins (HSPs) in the pathogenesis of atherosclerosis. Here, we explored the relationship between serum anti-HSP27 levels and a genetic variant, rs2868371, in the HSPB1 gene in the Stroke and Heart Atherosclerotic Disorders (MASHAD) cohort study carried out in Mashhad. A total of 8776 subjects were recruited. Anti-HSP27 levels were measured using an in-house enzyme-linked immunosorbent assay (ELISA), followed by genotyping using a TaqMan® probe-based assay. Demographic, biochemical, and hematological characteristics of the population were evaluated in all subjects. Kaplan-Meier curves were utilized, while logistic regression models were used to evaluate the relationship between genotypic frequencies and clinical characteristics of the population. No significant difference in anti-HSP27 levels was demonstrated between subjects with and without CHD. The frequencies of the CC, CG, and GG genotypes were 68%, 26.8%, and 5.2%, respectively. CAD patients with GG and GC genotypes had a lower risk of myocardial infarction (MI) compared with the reference group after adjusting for confounding factors [OR=0.27 (95% CI=0.08-0.94), P=0.040]. Our data revealed a relationship between the genetic variant in the HSPB1 gene and the risk of developing CHD, supporting further research on the potential value of this emerging marker in predicting https://acta.tums.ac.ir/index.php/acta/article/view/11152 https://acta.tums.ac.ir/index.php/acta/article/download/11152/6073