Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 23 1-2 1981 06 15 27 43 AHMAD SCHAHBAZFAR 2015 09 28 Blood specimens from 22 insulin dependent diabetic patient and 17 nontliabetic subject were collected im EDTA- tubes. Quantitation of Hb-Al was performed by Columne chromatography. The glycosylated hemoglobin component is increased in diabetic patient and found a significant correlation between Hb-Al concentration and blood glucose levels in all diabetic patients. The mean Hb-Al valve for diabetic patients is twice as the mean valve for nondiabetics. The second part of experiment consist of aging bloodEDTA in ambient temerature and a 4c cold room. A sample of each blood was analysed the following days for estimation of Hb-Al after 7 to 9 days and noticed adifferepe between the Hb-Al valves in two specimens and increase of Hb-Al in both samples but after aging forthermore about 19 days, the concentration of Hb-Al in blood kept in ambient temperature was three times more than in blood kept in cold room. https://acta.tums.ac.ir/index.php/acta/article/view/147 https://acta.tums.ac.ir/index.php/acta/article/download/147/143