Tehran University of Medical Sciences
Acta Medica Iranica
0044-6025
39
3
2001
09
15
Determination of total L-Ascorbic Acid by high performance liquid chromatography in human plasma
159
163
Oveisi MR
Sadeghi N
2015
09
28
The total vitamin C content in human plasma is widely accepted as an indicator of the tissue status of vitamin C. A liquid chromatography method with ultraviolet detector (264 nm) for measuring ascorbic acid in human plasma was developed. A C18 reversed-phase column and cetrimide as an ion-pairing agent was employed. Ascorbic acid (AA) was measured after reducing L-dehydroascorbic acid to L-ascorbic acid with dithiothreitol. The stability of the ascorbic acid in plasma, metaphosphoric acid and trichloroacetic acid was also evaluated. The analytical parameters, including linearity (1-60 µg/ml), accuracy (98.98%), repeatability (2.8%) and reproducibility (7.2%), showed that the method is reliable for measuring the total vitamin C content in plasma.
https://acta.tums.ac.ir/index.php/acta/article/view/2427
https://acta.tums.ac.ir/index.php/acta/article/download/2427/2416
d with the main procedures (MIC and MBC), on 66 strains obtained from 100 clinical specimens. Five different antibiotics (penicillin, cefazolin, ampicillin, amoxicillin and vancomycin)
were employed in this study. In the case of penicillin, 47 sensitive strains and 19 highly resistant strains were obtained. No intermediate or low-resistant strain was found. The frequency of resistant strains against other antibiotics was found to be 10.6%, 7.5%, 18.1% and 0%, respectively. All strains were sensitive to vancomycin except for a low resistant one. Care should be taken to choose a suitable drug when being faced with a resistant strain. Vancomycin can be used with confidence to cure infections induced by penicilin-resistant S. pneumoniae.
https://acta.tums.ac.ir/index.php/acta/article/view/2724
https://acta.tums.ac.ir/index.php/acta/article/download/2724/2706