Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 43 1 2005 02 15 32 36 E. Safarzadeh F. Mostafavi M.T. Haghi Ashtiani 2015 09 28 Recognized as a reliable tool for assessing the hypothalamus-pituitary-adrenal (HPA) axis, measurement of salivary cortisol plays an important role in both the clinical and research settings. To establish a normative data, which forms the basis for the usage of this valuable parameter, we gathered 8:00 h saliva samples from 94 healthy individuals aged 6-14 years. Cortisol levels were measured by radioimmunoassay technique, using Orion Diagnostica's coated tube technology. Based on mean±2SD, we established a normal range for salivary cortisol concentrations in this age group: 1.69 - 12.81nmol/L. Considering a confidence interval for upper and lower limits, there was an upper limit equal to 11.42 -14.29 nmol/L and a lower limit of 1.21 - 2.25 nmol/L. Regarding the results of this study, cortisol levels were age dependent, and although with a low correlation coefficient, there was a positive correlation between cortisol levels and weight and height. There was no correlation with BMI and no sex difference was found. https://acta.tums.ac.ir/index.php/acta/article/view/2958 https://acta.tums.ac.ir/index.php/acta/article/download/2958/2939