Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 46 4 2008 08 15 283 286 V Rahimi-Movaghar Sina Trauma and Surgery Research Center, Sina Hospital, School of Medicine, Tehran University of Med A Yazdi Department of Neurosurgery, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Ira S Saadat Sina Trauma and Surgery Research Center, Sina Hospital, School of Medicine, Tehran University of Med 2015 09 28 The dysesthesia and paresthesia that occurs in laboratory rats after spinal cord injury (SCI) results in autophagia. This self-destructive behavior interferes with functional assessments in designed studies and jeopardizes the health of the injured rat. In this study, we evaluated role of saturated picric acid in the prevention of autophagia and self-mutilation. All rats were anesthetized with an intraperitoneal injection of a mixture of ketamine (100 mg/kg) and xylazine (10 mg/kg) for the SCI procedures. In the first 39 rats, no solution applied to the hind limbs, but in the next 26 cases, we smeared the saturated picric acid on the tail, lower extremities, pelvic, and abdomen of the rats immediately after SCI. In the rats without picric acid, 23 rats died following autophagia, but in the 26 rats with picric acid, there was no autophagia (P < 0.001). Picric acid side effects in skin and gastrointestinal signs such as irritation, redness and diarrhea were not seen in any rat. Saturated picric acid is a topical solution that if used appropriately and carefully, might be safe and effectively prevents autophagia and self-mutilation. When the solution is applied to the lower abdomen and limbs, we presume that its bitterness effectively prevents the rat from licking and biting the limb. https://acta.tums.ac.ir/index.php/acta/article/view/3483 https://acta.tums.ac.ir/index.php/acta/article/download/3483/3460