Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 47 5 2009 10 15 369 372 EN Amaneh Mohammadi Roushandeh Department of Anatomy, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Farnaz Sohrabvand VALI-e-ASR Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran. Saeid Amanpour VALI-e-ASR Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran. Parichehr Pasbakhsh Department of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Abdollah Sobhani Department of Animal Sciences, Faculty of Agriculture, University. of Mohghegh Ardabili, Ardabil, Iran. Hossein Nikzad Department of Anatomy, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran. Aligholi Sobhani Department of Anatomy and VALI-e-ASR Reproductive Health Research Center, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 2015 09 28 Important aspect of sperm function such as motility and capacitation appear to be mediated at least partially though hyaloronic acid (HA). Present study investigated effects of different doses of HA on sperm motility and vitality in human. Sperm was obtained from 20 male from IVF clinic in Imam Khomeini Hospital. Sperm motility and vitality in human semen was analyzed according to WHO criteria before and 4 hours after treatment with different doses of HA (0.750, 1000 and 1250 µg/ml). The results showed that in 1000 µg/ml the percent of stage 3 and 4 increased compare to control group. Percent of stage 1 and 2 decreased in group with 1000 µg/ml HA, there was an increase in the percentage of stage 3 and 4 and decrease in percentage of stage 1 and 2 compare to control. In the group treated with 1250 µg/ml stage 1 and 2 increased while stage 3 and 4 decreased. Vitality in all groups decreased except of the group treated with 1000 µg/ml HA. The group with 1250 µg/ml showed significantly decrease in vitality compare to fresh group (P < 0.05). The present study showed that the effects of HA on sperm motility and vitality is dose dependant and 1000 µg/ml HA had the effective role on sperm parameters. https://acta.tums.ac.ir/index.php/acta/article/view/3609 https://acta.tums.ac.ir/index.php/acta/article/download/3609/3585