Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 49 5 2011 05 15 319 324 EN Mohammad Mohammadi Kerman Oral and Dental Diseases Research Center. AND Department of Periodontics, Dental School, Kerman University of Medical Sciences, Kerman, Iran. Rasool Mofid Department of Periodontics, Dental School, Shaheed Beheshti University of Medical Sciences, Tehran, Iran. Mohammad Ali Shokrgozar National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran. 2015 09 28 Tissue-engineered gingival graft was used for regenerating facial gingiva around an implant at lower left first premolar area with insufficient attached gingiva. Engineered gingival graft was produced by mixing 250 ml bovine skin collagen with 250 ml nutritional medium containing human gingival fibroblasts (2×105). 3 months post-surgery, there were gains in the attached gingiva compared to pre-surgery. The histological examination revealed a keratinized tissue on the treated site. Based on the result of this investigation, this graft was safe and capable of generating keratinized gingiva. https://acta.tums.ac.ir/index.php/acta/article/view/3749 https://acta.tums.ac.ir/index.php/acta/article/download/3749/3724