Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 48 5 2010 10 15 279 282 EN Reza Shahsiah Department of Pathology, Imam Hospitals Complex, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Alireza Abdollahi Department of Pathology, Imam Hospitals Complex, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Farid Azmoudeh Ardalan Department of Pathology, Imam Hospitals Complex, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Taghi Haghi-Ashtiani Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. Issa Jahanzad Department of Pathology, Imam Hospitals Complex, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Mohsen Nassiri Toosi Department of Gastroenterology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 2015 09 28 Fluorescent monitoring of DNA amplification is the basis of real-time PCR. Absolute quantification can be achieved using a standard curve method. The standard curve is constructed by amplifying known amounts of standards under identical conditions to that of the samples.The objective of the current study is to propose a mathematical model to assess the acceptability of PCR resulys.Four commercial standards for HCV-RNA (hepatitis C virus RNA) along with 6 patient samples were measured by real-time PCR, using two different RT-PCR reagents. The standard deviation of regression (Sy,x) was calculated for each group of standard and compared by F-Test. The efficiency kinetics was computed by logistic regression, c2 goodness of fit test was preformed to assess the appropriateness of the efficiency curves.Calculated efficiencies were not significantly different from the value predicted by logistic regression model. Reactions with more variation showed less stable efficiency curves, with wider range of amplification efficiencies.Amplification efficiency kinetics can be computed by fitting a logistic regression curve to the gathered fluorescent data of each reaction. This model can be employed to assess the acceptability of PCR results calculated by standard curve method. https://acta.tums.ac.ir/index.php/acta/article/view/4048 https://acta.tums.ac.ir/index.php/acta/article/download/4048/4023