Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 48 5 2010 10 15 283 288 EN Mehrdad Hashemi Department of Genetics, Islamic Azad University, Tehran Medical Branch, Tehran, Iran. Mehdi Nouri Long Department of Medicine, Islamic Azad University, Tehran medical Branch, Tehran, Iran. Maliheh Entezari Department of Genetics, Islamic Azad University, Tehran Medical Branch, Tehran, Iran. Shohreh Nafisi Department of Chemistry, Islamic Azad University, Central Tehran Branch, Tehran, Iran. AND Research Institute for Islamic and Complementary Medicine, Iran University of Medical Science, Tehran, Iran. Hossein Nowroozii Research Institute for Islamic and Complementary Medicine, Iran University of Medical Science, Tehran, Iran. 2015 09 28 Diet can play a vital role in cancer prevention. Nowadays the scientists are looking for food materials which can potentially prevent the cancer occurrence. The purpose of this research is to examine anti-mutagenic and apoptotic effects of apigenin in human lymphoma cells. In present study human chronic lymphocytic leukemia (Eheb cell line) were cultured in RPMI 1640 (Sigma), supplemented with 10% fetal calf serum, penicillin-streptomycin, L-glutamine and incubated at 37 ºC for 2 days. In addition cancer cell line was treated by and apigenin and cellular vital capacity was determined by MTT assay. Then effect of apigenin in human lymphoma B cells was examined by flow cytometry techniques. The apigenin was subsequently evaluated in terms of anti-mutagenic properties by a standard reverse mutation assay (Ames test). This was performed with histidine auxotroph strain of Salmonella typhimurium (TA100). Thus, it requires histidine from a foreign supply to ensure its growth. The aforementioned strain gives rise to reverted colonies when expose to sodium azide as a carcinogen substance. During MTT assay, human chronic lymphocytic leukemia revealed to have a meaningful cell death when compared with controls (P<0.01) Apoptosis was induced suitably after 48 hours by flow cytometry assay. In Ames test apigenin prevented the reverted mutations and the hindrance percent of apigenin was 98.17%.These results have revealed apigenin induced apoptosis in human lymphoma B cells in vitro. https://acta.tums.ac.ir/index.php/acta/article/view/4049 https://acta.tums.ac.ir/index.php/acta/article/download/4049/4024