Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 53 9 2015 10 06 540 546 EN Bagher Larijani Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran Iran. Hamidreza Aghayan cGMP Grade Stem Cell Facility, Chronic Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran. Parisa Goodarzi School of Nursing and Midwifery, Iran University of Medical Sciences, Tehran, Iran. Fereshteh Mohamadi-Jahani Department of Biology, Payame Noor University, Tehran, Iran. Abbas Norouzi-Javidan Brain and Spinal Cord Injury Research Center, Cellular Fanavaran Knowledge-Based Organization, Tehran University of Medical Sciences, Tehran, Iran. Ahmad Reza Dehpour Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Khadijeh Fallahzadeh cGMP Grade Stem Cell Facility, Chronic Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran. Forough Azam Sayahpour Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. Kazem Bidaki Department of Biology, Payame Noor University, Tehran, Iran. Babak Arjmand Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran. 2015 10 06 2015 10 06 In this study, our aim was to produce a generation of GMP-grade adipose tissue-derived mesenchymal stem cells for clinical applications. According to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cGMP) compliant adipose tissue-derived mesenchymal stem cells from five female donors. The isolated cells were cultured in DMEM supplemented with 10% fetal bovine serum and characterized by standard methods. Moreover, karyotyping was performed to evaluate chromosomal stability. Mean of donors’ age was 47.6 ± 8.29 year, mean of cell viability was 95.6 ± 1.51%, and cell count was between 9×106 and 14×106 per microliter with the mean of 12.2×106 ± 2863564.21 per microliter. The main aim of this project was demonstrating the feasibility of cGMP-compliant and clinical grade adipose tissue-derived mesenchymal stem cells preparation and banking for clinical cell transplantation trials. https://acta.tums.ac.ir/index.php/acta/article/view/4290 https://acta.tums.ac.ir/index.php/acta/article/download/4290/4244