Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 56 7 2018 08 13 434 440 EN Ala Habibian Infectious and Tropical Diseases Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, Jundishapur University of Medical Sciences, Ahvaz, Iran. Manoochehr Makvandi Infectious and Tropical Diseases Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, Jundishapur University of Medical Sciences, Ahvaz, Iran. Alireza Samarbaf-Zadeh Infectious and Tropical Diseases Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, Jundishapur University of Medical Sciences, Ahvaz, Iran. Niloofar Neisi Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran. Rahim Soleimani-Jelodar Infectious and Tropical Diseases Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran. Kamyar Makvandi Department of Orthopedic Surgery, Taleghani Hospital Research Development Unit, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Shima Izadi Infectious and Tropical Diseases Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran. 2017 07 16 2017 12 18 Epstein-Barr virus (EBV) is a DNA virus which belongs to the Herpesviridae family, has been infected with 90% of the world’s population. EBV is transmitted through saliva and associated with different diseases such as Infectious Mononucleosis, Nasopharyngeal Carcinoma, Burkitt`s lymphoma, Hodgkin and Non-Hodgkin`s lymphoma. This study was designed to determine the frequency of EBV DNA and genotyping of this virus in histological tissues of Hodgkin and Non-Hodgkin's lymphoma in Ahvaz, Iran. In this study, 12 samples of Hodgkin and 29 samples of Non-Hodgkin`s lymphoma were examined from Ahvaz Imam Khomeini Hospital. After deparaffinization and DNA extraction, Nested-PCR technique was carried out on Epstein-Barr nuclear antigen 1 (EBNA-1) and Epstein-Barr nuclear antigen 3C (EBNA-3C) regions in order to detect EBV genome and genotype in tumoral tissues. Among 12 cases of Hodgkin`s lymphoma, 3 (25%) cases and among 29 cases of Non-Hodgkin's lymphoma, 14 (48%) cases were positive for EBV. All 3 EBV positive cases of Hodgkin`s lymphoma and a number of 8 (57%) cases of Non-Hodgkin`s lymphoma belonged to the adolescence age group which proves an association between age and EBV positive in Hodgkin and Non-Hodgkin`s lymphoma according to Fisher`s exact test (P=0.03). All EBV positive cases in Hodgkin and Non-Hodgkin`s lymphoma were EBV-1. The results of this study revealed that EBV played a possible important role in Hodgkin and Non-Hodgkin`s lymphoma especially in adults. Also, the main genotype of virus in the current study was EBV-1. https://acta.tums.ac.ir/index.php/acta/article/view/6605 https://acta.tums.ac.ir/index.php/acta/article/download/6605/5126