Angiotensin 1-7 Administration Increases Renal Blood Flow in the Absence of Bradykinin B2 Receptor in Ovariectomized Estradiol Treated Rats: The Role of Mas Receptor

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 57 2 2019 03 12 Angiotensin 1-7 Administration Increases Renal Blood Flow in the Absence of Bradykinin B2 Receptor in Ovariectomized Estradiol Treated Rats: The Role of Mas Receptor 103 109 EN Shadan Saberi Department of Physiology, Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. AND Department of Physiology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran. Aghdas Dehghani Department of Physiology, Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. ANDDepartment of Physiology, School of Medicine, Hormozgan University of Medical Sciences, Bandar-Abbas, Iran. Mehdi Nematbakhsh Department of Physiology, Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. AND Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. AND IsfahanMN Institute of Basic and Applied Sciences Research, Isfahan, Iran. 2018 03 03 2018 12 15 Renin angiotensin (RAS), kallikrein kinin (KKS), and sex hormonal systems demonstrate a complex contribution in kidney circulation. This study was designed to investigate the role of angiotensin 1-7 (Ang 1-7) receptor (MasR) and of bradykinin B2 receptor (B2R) in renal blood flow (RBF) response to Ang 1-7 infusion in ovariectomized estradiol treated rats. The ovariectomized rats received intramuscular vehicle (group 1, OV) or estradiol valerate (500 µg/Kg/week) (group 2, OVE) for two weeks. Then each group was divided into two subgroups subjected to receive B2R antagonist (HOE-140, subgroup A), or MasR antagonist (A779) plus HOE-140 (subgroup B). RBF and renal vascular resistance (RVR) responses to graded Ang 1-7 infusion were determined. In condition of B2R alone blocking, RBF response to Ang 1-7 in OVE group was significantly greater than that of OV group (P=0.05), however this response difference was failed by co-blockades of MasR and B2R. Estradiol could promote RBF response to graded Ang 1-7 infusion in the absence of B2R alone, however when both receptors (MasR and B2R) were blocked the role of estradiol was limited. https://acta.tums.ac.ir/index.php/acta/article/view/7084 https://acta.tums.ac.ir/index.php/acta/article/download/7084/5218