Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 58 3 2020 07 19 130 133 Atousa Hakamifard Department of Infectious Diseases, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Rasool Soltani Department of Clinical Pharmacy, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran. Ali Hjigholami Department of Hematology-Oncology, Isfahan University of Medical Sciences, Isfahan, Iran. Sholeh Yaghoubi Department of Infectious Diseases, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. 2019 05 09 2020 04 24 Epstein-Barr virus (EBV) is a member of the herpes virus family and is characterized by fever, lymphadenopathy, and sore throat. In this report, we present a 20-year-old woman with a four-day history of fever, chills, nausea, vomiting, jaundice and abdominal pain, and a diagnosis of acalculous cholecystitis, acute hepatitis with hemolytic anemia due to infectious mononucleosis. After the confirmation of acute acalculous cholecystitis along with hemolytic anemia, prednisolone was initiated for the patient. Supportive measures resolved the symptoms of acalculous cholecystitis. https://acta.tums.ac.ir/index.php/acta/article/view/7866 https://acta.tums.ac.ir/index.php/acta/article/download/7866/5384