Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 60 11 2023 01 10 699 706 Sahar Zare Health Information Management Research Center (HIMRC), Kashan University of Medical Sciences, Kashan, Iran Mohammad Shirdeli Department of Health Information Technology, Human Resources Development Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Rita Rezaee Department of Health Information Management, Health Human Resources Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Fatemeh Niknam Department of Health Information Technology, Human Resources Development Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Sara Mobarak Department of Infectious Disease, School of Medicine, Abadan University of Medical Sciences, Abadan, Iran Saeed Jelvay Department of Health Information Management, Abadan University of Medical Sciences, Abadan, Iran 2021 11 25 2022 08 19 The successful implementation of mobile learning is mainly impacted by learners’ attitudes to accepting and applying mobile learning systems. In the current study, an m-learning application was developed and implemented for university students, and their perspective toward the adoption and application of m-learning was investigated. This cross-sectional study was carried out in 2020. The participants were 114 university students at Abadan University of Medical Sciences. Data were collected by means of a valid questionnaire containing 42 questions in 13 subscales in addition to an open-ended question about the positive and negative aspects of the m-learning application. There was a significant relationship between acceptance and major (P=0.001), mobile usage (P=0.035), and familiarity with m-learning (P<0.001). The total mean of m-learning application acceptance was 3.95±0.32, which shows a good level. Self-efficacy, perceived ease of use, and user interface were, respectively, the most influencing factors; mobile device limitation and governmental support were the least influencing factors. “Direct communication with the teacher/instructor” and “Internet disconnection during video teaching” were among the positive and negative aspects of applying the m-learning application, respectively. The m-learning application was well adopted among the students. Mobile device limitations are a reason for the student's concerns. “Usability features” can be a point for covering mobile device limitations. https://acta.tums.ac.ir/index.php/acta/article/view/9807