Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 55 5 2017 07 11 282 289 EN Soheila Sarmadi Department of Pathology, Women Hospital, Tehran University of Medical Sciences, Tehran, Iran. Narges Izadi-Mood Department of Pathology, Women Hospital, Tehran University of Medical Sciences, Tehran, Iran. Fatemeh Movarei Department of Pathology, Women Hospital, Tehran University of Medical Sciences, Tehran, Iran. Salma Sefidbakht Department of Pathology, Bahrami Hospital, Tehran University of Medical Sciences, Tehran, Iran. Kambiz Sotoudeh Department of Pathology, Hasheminejad Hospital, Iran University of Medical Sciences, Tehran, Iran. Eesa Jahanzad Department of Pathology, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran ,Iran. 2016 02 25 2017 01 30 Tumor angiogenesis is one of the most important factors in tumor progression. In this study, the angiogenesis of cervical squamous cell carcinoma (SCC) and its association with prognostic factors was assessed by using CD34 immunostaining marker. The microvessel density in 40 patients with cervical SCC was studied in three areas of the tumor; stromal and peripheral tumor area (combined) central stromal tumor area and peripheral tumor area and the relationship of microvascular density and survival was also evaluated. The count of CD34 is correlated with younger age, the presence of perineural invasion and metastasis to lymph nodes. High peripheral tumor angiogenesis is also correlated with lower disease-free tumor survival. According to the findings of the present study, CD34 expression, especially in peripheral tumor areas, can be used as a prognostic marker in cervical SCC. https://acta.tums.ac.ir/index.php/acta/article/view/5468 https://acta.tums.ac.ir/index.php/acta/article/download/5468/4933

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 55 5 2017 07 11 340 343 EN Kamran Balighi Department of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran. Arghavan Azizpour Department of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran. Ali Sadeghinia Department of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran. Vahide Saeidi Department of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran. 2015 10 20 2016 09 27 Paraneoplastic pemphigus (PNP) is an autoimmune bullous disease associated with underlying neoplasms, both malignant and benign. The most constant clinical presentation of PNP is the presence of intractable stomatitis. Herein we present a 25-year-old male with a 3-month history of refractory stomatitis especially involving the lips and widespread vesiculobullous eruption on his trunk and extremities. The diagnosis of PNP was confirmed based on histological and serological results. Investigation for the underlying neoplasm revealed a retroperitoneal tumorous mass which was biopsied and diagnosed as the inflammatory myofibroblastic tumor (IMT). The tumor was surgically excised, and different treatment regimens were used to treat the mucocutaneous lesions. Skin lesions responded favorably to treatment, but oral stomatitis still persists which is the case in most PNP patients. This combination of PNP and IMT has rarely been reported in the literature. Treatment started with corticosteroid and rituximab then tumor excised. https://acta.tums.ac.ir/index.php/acta/article/view/5011 https://acta.tums.ac.ir/index.php/acta/article/download/5011/4941

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 55 5 2017 07 11 352 353 EN Mirhojjat Khorasanizadeh Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. Mahsa Eskian Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. Nima Rezaei Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. AND Network of Immunity in Infection, Malignancy and Autoimmunity (NIIMA), Universal Scientific Education and Research Network (USERN), Tehran, Iran. 2016 04 01 2017 01 03 https://acta.tums.ac.ir/index.php/acta/article/view/5532 https://acta.tums.ac.ir/index.php/acta/article/download/5532/4944

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 55 5 2017 07 11 290 296 EN Majid Mossahebi-Mohammadi Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Amir Atashi Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. AND Stem Cell and Tissue Engineering Research Center, Shahroud University of Medical Sciences, Shahroud, Iran. Saeid Kaviani Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Masoud Soleimani Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. 2017 01 21 2017 02 21 Hematopoietic stem cells (HSCs) were characterized by self-renewal and multilineage potential. Umbilical cord blood-derived (UCB) as an alternative source of HSCs is widely used especially in children for stem cells transplant (SCT). The main limitation in using UCB for transplantation especially in adults is low cell dose. To overcome this limitation besides using double dose UCB, ex vivo expansion is the most important way to increase cell number for transplantation. HSCs are mainly isolated using CD133 or CD34. CD133, as the most primitive marker, shows important physiological role in maintenance and expansion of HSCs. SALL4 plays crucial role in the development and maintaining the pluripotency and self-renewal ability of embryonic stem cells (ESCs) as well as HSCs. Moreover, SALL4 act as a regulator of HSCs expansion, normal hematopoiesis, and hematological malignancies. In the present study, CD133+ cells positively selected and ex vivo expanded in SALL-4 and GFP-transduced group. CD133 expression assessed using flow cytometry at day 0, 7 and 10. Moreover, multilineage differentiation and proliferation potential of expanded cells in both groups evaluated using colony forming unit (CFU) assay, and cells count assay. Karyotyping analysis was performed to assess any chromosomal instability after 7 days of expansion. Obtained results demonstrated that SALL-4 transduced cells showed significant increase in cell number compared to control group. Moreover, immunophenotyping results showed higher expression level of CD133 at day 7 and 10 following expansion in SALL-4 transduced (62 % and 42%) compared to control group (51% and 20.6%). Our results illustrated that SALL4 could act as a positive factor for the expansion of CD133+ derived UCB cells besides maintaining self-renewal and differentiation ability of expanded cell without any numerical and structural chromosomal aberrations. https://acta.tums.ac.ir/index.php/acta/article/view/6161 https://acta.tums.ac.ir/index.php/acta/article/download/6161/4934

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 55 5 2017 07 11 344 347 EN Hamid Attaran Legal Medicine Research Center, Legal Medicine Organization, Mashhad, Iran. 2016 04 08 2016 10 21 Methamphetamine is one of the most common abused drugs, so its various effects on different body organs should be familiar to all physicians. Regarding its gastrointestinal sequels, there are few reports of ischemic colitis induced by its vasoconstrictive effects. This is the first report of isolated small intestinal infarction resulting in death following methamphetamine toxicity. A 40-year-old woman with a past history of medical treatment for obesity referred to hospital with severe chest and back pain, perspiration, nausea, agitation, high blood pressure, bradycardia and subsequent lethargy and vasomotor instability. Cardiac evaluations were normal, and a toxicologic urinalysis revealed methamphetamine. Later, abdominal pain predominated, and ultrasonography revealed signs of bowel infarction. She did not consent to surgery and succumbed afterward. At autopsy gangrene and perforation of distal ileum were found. The cause of death was determined as intestinal gangrene following methamphetamine toxicity. Methamphetamine has anorectic effects and so is used in some "diet pills"; Consumers may not even know they are using methamphetamine. Hence in cases of either known MA abuse or those using unknown weight reduction drugs presenting with gastrointestinal complaints or abdominal pain, intestinal ischemia should be kept in mind and if plausible, intervened promptly. https://acta.tums.ac.ir/index.php/acta/article/view/5564 https://acta.tums.ac.ir/index.php/acta/article/download/5564/4942

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 55 5 2017 07 11 297 303 EN Arezou Ebrahimi Department of Biology, Faculty of Sciences, Islamic Azad University, Damghan Branch, Damghan, Iran. AND Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran. Abasalt Hosseinzadeh Colagar Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran. Mohammad Karimian Department of Biology, Faculty of Sciences, Islamic Azad University, Damghan Branch, Damghan, Iran. 2016 08 04 2017 01 29 Methionine synthase (MTR) is one of the key enzymes of folate pathway, which play a key role in the construction, repair, and methylation of DNA. In this study, an association of MTR A2756G gene transition with prostate cancer in men populations of Kashan-Iran was investigated by a case-control study and an in silico analysis. The 200 samples including 100 patients with prostate cancer, as case group and 100 healthy men, as control group included in this study. MTR-A2756G genotyping was performed by PCR-RFLP technique. Some in silico tools used to evaluate the effects of A2756G transition on the structure and function of MTR. Results showed that the AG genotype (OR: 2.4014, 95% CI: 1.3216-4.3636, P=0.0040), and GG genotype (OR: 3.6324, 95% CI: 1.2629-10.4475, P=0.0167) and G allele (OR: 2.0120, 95% CI: 1.3098-3.0905, P=0.0014) were associated with prostate cancer. In silico analysis showed that polymorphisms of the enzyme protein might change properties of MTR such as relative mutability and flexibility, which leads to alteration of stability and function of the enzyme. Based on the results, an MTR-A2756G polymorphism which changes activity and stability of the methionine synthase associated with prostate cancer in men. It is a preliminary study and is presenting data for future comprehensive study for making a clinical conclusion that this gene transition is a biomarker for susceptibility to prostate cancer. https://acta.tums.ac.ir/index.php/acta/article/view/5801 https://acta.tums.ac.ir/index.php/acta/article/download/5801/4935

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 55 5 2017 07 11 348 351 EN Hamid Mohammadi Kojidi Department of Forensic Medicine, School of Medicine, Razi Teaching Hospital, Guilan University of Medical Sciences, Rasht, Iran. Morteza Rahbar Taramsari Department of Forensic Medicine, School of Medicine, Razi Teaching Hospital, Guilan University of Medical Sciences, Rasht, Iran. Alireza Badsar Department of Forensic Medicine, School of Medicine, Razi Teaching Hospital, Guilan University of Medical Sciences, Rasht, Iran. Mirsaeed Attarchi Department of Forensic Medicine, Inflammatory Lung Disease Research Center, Guilan University of Medical Sciences, Rasht, Iran. 2016 02 22 2016 10 10 A 51-year-old man committed suicide by swallowing the contents of a fire extinguisher. A few hours after his suicide attempt, he was referred to the medical center for poisoning. At the time of admission, the patient was conscious with stable vital signs. The patient complained of burning lips and mouth, mentioning diarrhea. Initial treatments included gastric lavage with activated charcoal, while paraclinical measures were requested. The patient had undergone hypernatremia (Na: 152 mEq/l) and metabolic alkalosis. Treatment focused on the adjustment of sodium level and alkalosis. On the first day of hospitalization, the patient experienced recurrent episodes of tonic-clonic seizure along with the loss of consciousness. On the third day of hospitalization, the patient developed respiratory arrest followed by cardiac arrest and death. https://acta.tums.ac.ir/index.php/acta/article/view/5462 https://acta.tums.ac.ir/index.php/acta/article/download/5462/4943

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 55 5 2017 07 11 304 310 EN Asie Sadeghi Department of Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran. Maani Beigy Students' Scientific Research Center (SSRC), Tehran University of Medical Sciences, Tehran, Iran. Samira Alizadeh Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Hossein Mazloom Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Sanaz Vakili Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. https://acta.tums.ac.ir/index.php/acta/article/view/7057 https://acta.tums.ac.ir/index.php/acta/article/download/7057/5222

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 57 2 2019 03 12 127 133 EN Shahin Koohmanaee Department of Pediatrics, Pediatric Diseases Research Center, Guilan University of Medical Sciences, Rasht, Iran. Afagh Hassanzadeh Rad Pediatric Diseases Research Center, Guilan University of Medical Sciences, Rasht, Iran. Seyyedeh Forough Jafari Pediatric Diseases Research Center, Guilan University of Medical Sciences, Rasht, Iran. Marjaneh Zarkesh Department of Neonatology, Pediatric Diseases Research Center, Guilan University of Medical Sciences, Rasht, Iran. Ehsan Kazemnejad Leili Department of Biostatistics, School of Nursing, Guilan University of Medical Sciences, Rasht, Iran. Esfandiar Nazari Department of Pediatrics, Pediatric Diseases Research Center, Guilan University of Medical Sciences, Rasht, Iran. Setila Dalili Department of Pediatrics, Pediatric Diseases Research Center, Guilan University of Medical Sciences, Rasht, Iran. 2018 03 17 2018 12 05 We aimed to define Metabolic Syndrome (METs) from different viewpoints to determine the most appropriate method that could be used for early METs' diagnosis in general population and treat them immediately. This study was an analytic cross-sectional study which was conducted on 725, twelve year-old-girls and boys from Rasht city in Iran. METs was defined based on 7 different methods. Data were reported by descriptive statistics (number, percent, mean, and standard deviation) and analyzed by Cohen's kappa coefficient correlation and chi-square in SPSS version 19. The highest and lowest percentages of METs were obtained by DE Ferranti (17.5%) and viner et al., (0.8%) methods, respectively. Results showed that viner et al., had the highest degree of agreement with NCEP ATPIII and the lowest with DE Ferranti. Furthermore, De Ferranti showed the highest degree of agreement with NHANESIII and the lowest with Viner et al., According to results, the identification of the cut off points of obesity could help to promote public health care. https://acta.tums.ac.ir/index.php/acta/article/view/7124 https://acta.tums.ac.ir/index.php/acta/article/download/7124/5223

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 57 2 2019 03 12 134 137 EN Leila Pourali Department of Obstetrics and Gynecology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Farokh Seilanian Toosi Department of Radiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Atiyeh Vatanchi Department of Obstetrics and Gynecology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Ali Taghizadeh Surgical Oncology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Zahra Rastin Department of Obstetrics and Gynecology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. 2018 07 25 2018 11 10 Ovarian tumors are rare in childhood and adolescent age. A 14-year-old girl presented with abdominal distention and mild cyclical abdominal pain since 3 months ago. There was an abdominal distention, and huge firm mass was palpated from pelvis to epigastric region. Abdominal ultrasonography revealed normal uterus and large multiloculated adnexal mass with multiple fine septations. Laparotomy was performed, and the ovarian mucinous borderline tumor was reported in frozen section biopsy. Exploration of other abdominopelvic organs revealed no other pathological signs. The final pathological report showed the right ovarian mucinous borderline tumor. Although the mucinous ovarian borderline tumor is a rare condition in adolescent age, pelvic mass, especially with solid or nodular component, must arise this diagnosis, and exploratory laparotomy with comprehensive surgical staging with regard to fertility preservation is warranted. https://acta.tums.ac.ir/index.php/acta/article/view/7403 https://acta.tums.ac.ir/index.php/acta/article/download/7403/5224

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 57 2 2019 03 12 138 140 EN Moein Moghaddam Ahmadi Student Research Committee, Guilan University of Medical Sciences, Rasht, Iran. Mohammad Javad Ghanbarkhah Student Research Committee, Guilan University of Medical Sciences, Rasht, Iran. Hossein Mohammadi Student Research Committee, Guilan University of Medical Sciences, Rasht, Iran. Marzieh Gholizadeh Student Research Committee, Guilan University of Medical Sciences, Rasht, Iran. 2018 03 06 2018 12 15 Gastrointestinal Stromal Tumors (GISTs) are the most common gastrointestinal mesenchymal tumors and in 80% of the cases are associated with KIT activating mutations. The incidence of GISTs is generally 10-20 cases per million population, which turn out to be malignant in 20-30% of cases. GISTs can be large and present with dysphagia, even though they are usually small and asymptomatic. A 67-year-old woman was referred to the hospital complaining of pain and burning in epigastric area and left flank; the patient had experienced a weight loss within one month before the admission. Primary investigations upon upper endoscopy revealed a submucosal mass in distal part of esophagus, and nodularity and erythema in stomach. But through more examinations by MDCT of Abdomen and Pelvis, a mass measuring 20*33 mm in cardiac and lesser curvature submucosa, without lymphadenopathy was reported, that could suggest GIST. This mass was excised with normal margins via wide local excision, bilayer cardiplasty and funduplication. The patient was hospitalized for 10 days after surgery, and was discharged with good general condition. In follow-up, there wasn’t any sign of dysphagia and dyspepsia within 24 months. In the patients suffering from GIST, the tumor location appears to have the most important role on deciding whether to perform local excision or not. Treatment of GISTs locating near gastroesophageal junction (GE junction) is still challenging. Using an appropriate therapeutic method is crucial in improving the patient’s quality of life. Therefore, wide local excision followed by bilayer cardiplasty and funduplication, could be considered as the appropriate therapeutic protocol in such same situations due to elimination of post total gastrectomy adverse effects and the tumor relapse. https://acta.tums.ac.ir/index.php/acta/article/view/7094 https://acta.tums.ac.ir/index.php/acta/article/download/7094/5225

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 57 2 2019 03 12 141 142 EN Hesam Jahandideh Department of Otolaryngology-Head and Neck Surgery, Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran. Farideh Hosseinzadeh ENT and Head and Neck Research Center, The Five Senses Institute, Iran University of Medical Sciences, Tehran, Iran. 2018 10 12 2018 11 14 Nasal foreign bodies are usually received in otolaryngology practice. Although more frequently seen in pediatric patients, also they can affect adults, specifically those with mental retardation or any psychiatric problems. We presented an unusual case of the nasal foreign body, an eraser rhinolith in a 17-year-old boy with mild mental retardation presented with long-lasting nasal obstruction but no chronic infection or epistaxis. Computed tomography revealed a peripherally calcified sub-mucosal round mass in the left nasal cavity. After surgery, a round shape foreign body that looked like an eraser piece was removed from the nasal cavity. Rhinolith can present just with nasal obstruction. With properly diagnosed and appropriate surgery, all rhinoliths can be removed and complication of extraction can be minimized. https://acta.tums.ac.ir/index.php/acta/article/view/7526 https://acta.tums.ac.ir/index.php/acta/article/download/7526/5226

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 57 2 2019 03 12 143 146 EN Souhir Khemiri Department of Medical Oncology, CHU Habib Bourguiba, Sfax, Tunisia. Jihene Feki Department of Medical Oncology, CHU Habib Bourguiba, Sfax, Tunisia. Afef Khanfir Department of Medical Oncology, CHU Habib Bourguiba, Sfax, Tunisia. Mohamed Abdelmoula Department of Maxillofacial Surgery, CHU Habib Bourguiba, Sfax, Tunisia. Mounir Frikha Department of Medical Oncology, CHU Habib Bourguiba, Sfax, Tunisia. 2018 01 31 2018 11 05 Melanotic neuroectodermal tumor of infancy (MNTI) is a rare and distinctive neoplasm of early infancy with rapid expansile growth and a high rate of recurrences. Most commonly the lesion affects the maxilla of infants during the first year of life, but it may also occur in the mandible, skull, brain, epididymis, and other rare locations. Common treatment methods’ include surgical excision and resection of the tumor. The aim of this article was to show the diagnosis and treatment of a 7-month-old patient with melanotic neuroectodermal tumor occurred in the anterior mandible and to demonstrate the effectiveness of the neoadjuvant chemotherapy. https://acta.tums.ac.ir/index.php/acta/article/view/7005 https