Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 56 7 2018 08 13 421 428 EN Mina Afhami Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran. Mansoureh Hashemi Department of Neuroscience, Functional Neurosurgery Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Khashayar Afshari Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran. Jamal Chenari Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Seyed Mohammad Tavangar Department of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran. Mahmoudreza Hadjighassem Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. Ahmad Reza Dehpour Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Ahad Muhammadnejad Cancer Biology Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran. Arvin Haj-Mirzaian Department of Pharmacology, Tehran University of Medical Sciences, Tehran, Iran. AND Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran. Vafa Rahimi-Movaghar Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran. AND Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Neurosurgery, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran. 2017 10 02 2018 03 13 Olfactory ensheathing cells-based therapy for spinal cord injury (SCI) repair has been a possible treatment for clinical study because of their safety in autologous transplantation and potential regenerative capability. However, there are contradictory reports on the results after transplantation in animal models. The purpose of this research was to investigate the effect of acute transplantation of human mucosa-derived olfactory ensheathing cells (OECs) on the repair of the spinal cord. Human olfactory ensheathing cells were isolated from the human mucosa and cultured under supplemented neuronal cell culture medium. They were characterized by immunocytochemistry for olfactory ensheathing cell markers. We induced spinal cord injury at T8-T9 of rats by aneurysm clips and simultaneously injected two million OECs into subarachnoid space of spinal cord. Sensory and motor behaviors were recorded by tail-flick reflex (TFR) and BBB scores, respectively every week for seven weeks after injury. Morphology and S100-beta antigen expression in olfactory ensheathing cells of the human olfactory mucosa was confirmed by immunostaining. OECs transplantation did not recover inflammation, neuronal vacuolation, hemorrhage, and cyst formation. These findings suggest that OECs transplantation in this experimental setting did not lead to tissue regeneration to enhance locomotion. These results broaden current knowledge and are additions to the science and literature. https://acta.tums.ac.ir/index.php/acta/article/view/6754 https://acta.tums.ac.ir/index.php/acta/article/download/6754/5124

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 56 7 2018 08 13 474 477 EN Saeid Morovvati Human Genetic Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Paniz Farshadyeganeh School of Basic Sciences, Tehran Medical Branch, Islamic Azad University, Tehran, Iran. Mojdeh Hamidizadeh School of Basic Sciences, Islamic Azad University Tehran Medical Branch, Tehran, Iran. Ziba Morovvati Department of Medical Genetics, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran. Samaneh Doost Mohammadi Faculty of Basic Sciences, Islamic Azad University of Zanjan, Zanjan, Iran. 2017 05 17 2017 12 18 Lipoid proteinosis (LP) is a rare autosomal recessive disorder caused by malfunction mutations in extracellular matrix protein 1 gene (ECM1) with common features such as hoarseness of the voice, infiltration of the skin and mucosa, and varying degrees of skin scars. We studied two LP patients. Clinical and genetic examination and genetic counseling were carried out, and their family pedigree was drawn. Two different variants were found in exon 6 of ECM1 gene in both patients: a homozygous deletion of a nucleotide T at position 507 and a missense variant at nucleotide 389 which the first was a pathogenic mutation and the other one was a non-pathogenic variant. https://acta.tums.ac.ir/index.php/acta/article/view/6466 https://acta.tums.ac.ir/index.php/acta/article/download/6466/5131

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 56 7 2018 08 13 482 483 EN Hussein Baharlooi Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Nima Rezaei Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. AND Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran. AND Network of Immunity in Infection, Malignancy and Autoimmunity (NIIMA), Universal Scientific Education and Research Network (USERN), Tehran, Iran. Ehsan Sharif-Paghaleh Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. AND Division of Imaging and Biomedical Engineering, School of Life Sciences and Medicine, King’s College London, London, United Kingdom. 2017 12 10 2018 03 02 No Abstract https://acta.tums.ac.ir/index.php/acta/article/view/6883 https://acta.tums.ac.ir/index.php/acta/article/download/6883/5134

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 56 7 2018 08 13 429 433 EN Isaura Isabelle Fonseca Gomes-Silva Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235-Cidade Universitária, Recife-PE, Brazil. Eliezer Rushansky Department of Clinical Rheumatology, University of Pernambuco, Rua Arnóbio Marques, 10-Santo Amaro, Recife-PE, Brazil. Francisco Geraldo Carvalho-Neto Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235-Cidade Universitária, Recife-PE, Brazil. Amanda Virginia Batista Vieira Department of Biology, Rural Federal University of Pernambuco, Rua Manoel de Medeiros-Dois Irmãos, Recife-PE, Brazil. Maria Helena Queiroz de Araujo Mariano Department of Clinical Rheumatology, University of Pernambuco, Rua Arnóbio Marques, 10-Santo Amaro, Recife-PE, Brazil. Paulo Roberto Eleutério de Souza Department of Biology, Rural Federal University of Pernambuco, Rua Manoel de Medeiros-Dois Irmãos, Recife-PE, Brazil. Martín Alejandro Montes Department of Biology, Rural Federal University of Pernambuco, Rua Manoel de Medeiros-Dois Irmãos, Recife-PE, Brazil. Maria Mascena Diniz Maia Department of Biology, Rural Federal University of Pernambuco, Rua Manoel de Medeiros-Dois Irmãos, Recife-PE, Brazil. 2017 04 08 2017 12 12 Rheumatoid Arthritis (RA) is a complex disease with higher level of IL-6. In order to elucidate the alterations related to IL-6 gene in RA, we evaluated the -174 G/C IL-6 gene polymorphism and methylation pattern of its promoter. A total of 120 RA patients and 120 healthy controls were recruited for polymorphism analysis, and 30 individuals of both groups were randomly selected for methylation analysis. The IL-6 gene polymorphism was analyzed by PCR-RFLP and methylation pattern was analyzed by MSP-PCR. Regarding IL-6 gene polymorphism, no significant difference was found between RA patients and controls (P>0.05). For DNA, two CpG regions of IL-6 promoter gene was analyzed. The first region (−1069, −1061, −1057 and −1001 CpG) did not show significant differences, whereas the second region (−628, −610, −574 and −491 CpG) showed a significant hypermethylated status (P=0.0004) in RA patients as compared with controls.These results, suggest a possible relationship between methylation pattern and the susceptibility to RA in our studied population. https://acta.tums.ac.ir/index.php/acta/article/view/6344 https://acta.tums.ac.ir/index.php/acta/article/download/6344/5125

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 56 7 2018 08 13 478 481 EN Leila Pourali Department of Obstetrics and Gynecology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Sedigheh Ayati Department of Obstetrics and Gynecology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Parvaneh Layegh Department of Radiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Atieh Vatanchi Department of Obstetrics and Gynecology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Zahra Rastin Department of Obstetrics and Gynecology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Azadeh Shourvi Department of Obstetrics and Gynecology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. 2017 07 09 2017 12 18 Persistent low level of beta-hCG (PLL) is defined as rising hCG level no more than two-fold over a three months period. Almost many types of PLL can lead to the wrong diagnosis. Here, we presented two cases of the retained product of conception (RPOC) with persistent low levels of beta-hCG. Both cases were presented with persistent low levels of beta-hCG and abnormal uterine bleeding since first-trimester pregnancy termination. Ultrasonography revealed a vascular mass with extension from the endometrial cavity to myometrium imitating gestational trophoblastic disease (GTD) or arteriovenous malformation (AVM). The final pathologies of both cases were retained product of conception. Imaging features of RPOC can closely imitate those of an AVM or GTN; so, hysteroscopy is one of the best non-invasive procedures which may be helpful in diagnosis and selection of appropriate treatment especially in young patients who desire to preserve their fertility.   https://acta.tums.ac.ir/index.php/acta/article/view/6589 https://acta.tums.ac.ir/index.php/acta/article/download/6589/5133

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 56 7 2018 08 13 434 440 EN Ala Habibian Infectious and Tropical Diseases Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, Jundishapur University of Medical Sciences, Ahvaz, Iran. Manoochehr Makvandi Infectious and Tropical Diseases Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, Jundishapur University of Medical Sciences, Ahvaz, Iran. Alireza Samarbaf-Zadeh Infectious and Tropical Diseases Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran. AND Department of Virology, Jundishapur University of Medical Sciences, Ahvaz, Iran. Niloofar Neisi Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran. Rahim Soleimani-Jelodar Infectious and Tropical Diseases Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran. Kamyar Makvandi Department of Orthopedic Surgery, Taleghani Hospital Research Development Unit, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Shima Izadi Infectious and Tropical Diseases Research Center, Health Research Institute, Jundishapur University of Medical Sciences, Ahvaz, Iran. 2017 07 16 2017 12 18 Epstein-Barr virus (EBV) is a DNA virus which belongs to the Herpesviridae family, has been infected with 90% of the world’s population. EBV is transmitted through saliva and associated with different diseases such as Infectious Mononucleosis, Nasopharyngeal Carcinoma, Burkitt`s lymphoma, Hodgkin and Non-Hodgkin`s lymphoma. This study was designed to determine the frequency of EBV DNA and genotyping of this virus in histological tissues of Hodgkin and Non-Hodgkin's lymphoma in Ahvaz, Iran. In this study, 12 samples of Hodgkin and 29 samples of Non-Hodgkin`s lymphoma were examined from Ahvaz Imam Khomeini Hospital. After deparaffinization and DNA extraction, Nested-PCR technique was carried out on Epstein-Barr nuclear antigen 1 (EBNA-1) and Epstein-Barr nuclear antigen 3C (EBNA-3C) regions in order to detect EBV genome and genotype in tumoral tissues. Among 12 cases of Hodgkin`s lymphoma, 3 (25%) cases and among 29 cases of Non-Hodgkin's lymphoma, 14 (48%) cases were positive for EBV. All 3 EBV positive cases of Hodgkin`s lymphoma and a number of 8 (57%) cases of Non-Hodgkin`s lymphoma belonged to the adolescence age group which proves an association between age and EBV positive in Hodgkin and Non-Hodgkin`s lymphoma according to Fisher`s exact test (P=0.03). All EBV positive cases in Hodgkin and Non-Hodgkin`s lymphoma were EBV-1. The results of this study revealed that EBV played a possible important role in Hodgkin and Non-Hodgan University of Medical Sciences, Tehran, Iran. Moloud Payab Obesity and Eating Habits Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran. Gholamreza Pourmand Urology Research Center, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran. 2017 03 13 2017 03 15 Stress urinary incontinence is a common medical problem among women. The urethral closure complex and/or the supportive mechanisms are responsible for incontinence in the majority of patients. Several surgical procedures with different degrees of invasiveness and outcomes have been reported to treat the problem. Although most of these procedures are reasonably effective, a general trend towards the study of natural and biocompatible tissues is emerging over popular synthetic materials. Here we report our experience of autologous adipose-derived stem cells transplantation into the periurethral region as a new method of stress urinary incontinence treatment. Ten women with symptoms of stress urinary incontinence were treated by injections of autologous adipose-derived stem cells into the periurethral region via transurethral and transvaginal approach under urethroscopic observation. This report presents the short-term outcome of the patients. The outcome measured by pad test results, ICIQ-SF scores, and Qmax. The mean age of the participants was 45.8±8.7 years. Urinary incontinence significantly decreased through the first two, 6 and 24 weeks after the injection therapy. The difference was significant in pad test results (P<0.001) and ICIQ-SF scores (P<0.001), especially comparing results between 2 and 6 weeks and among 6 and 24 weeks, but not for 2 and 6 weeks compared to each other. Surprisingly, Qmax showed improvement after the study period (means 32.6 vs. 35.7; P=0.002). This study showed that injection of the autologous adipose-derived stem cells to the periurethral region is a safe, yet short-term effective treatment option for stress urinary incontinence. Further studies with longer follow up are needed to confirm its long term efficacy. https://acta.tums.ac.ir/index.php/acta/article/view/6295 https://acta.tums.ac.ir/index.php/acta/article/download/6295/4947

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 55 6 2017 08 19 411 413 EN Raheleh Aliabadi Rasoul-e-Akram General Hospital, Iran University of Medical Sciences, Tehran, Iran. Hassan Riahi Beni Rasoul-e-Akram General Hospital, Iran University of Medical Sciences, Tehran, Iran. Jila Nadjafi East Surrey Associate Hospital for Brighton University, London, Uk. Morteza Hassanzade Rasoul-e-Akram General Hospital, Iran University of Medical Sciences, Tehran, Iran. Mehrnaz Tabrizi Rasoul-e-Akram General Hospital, Iran University of Medical Sciences, Tehran, Iran. Mohsen Nasiri Rasoul-e-Akram General Hospital, Iran University of Medical Sciences, Tehran, Iran. Gandom Sedehi Rasoul-e-Akram General Hospital, Iran University of Medical Sciences, Tehran, Iran. Ali Pourmojarab Rasoul-e-Akram General Hospital, Iran University of Medical Sciences, Tehran, Iran. 2015 10 28 2016 04 17 Streptokinase is a fibrinolytic agent that enhances plasmin activation and is used in selected patients with acute ST elevation myocardial infarction (STEMI). Similar to the other thrombolytics, a common side effect is bleeding, especially from venous puncture sites. Here, we present a case of acute anterior wall STEMI complicated by large spontaneous iliopsoas hematoma after streptokinase administration. With conservative management, the course of the disease was uneventful, and the patient was discharged with no symptom and no clinically important sequel. https://acta.tums.ac.ir/index.php/acta/article/view/5087 https://acta.tums.ac.ir/index.php/acta/article/download/5087/4955

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 55 6 2017 08 19 375 380 EN Abbas Darjani Department of Dermatology, Skin Research Center, Guilan University of Medical Sciences, Rasht, Iran. Nahid Nickhah Department of Dermatology, Skin Research Center, Guilan University of Medical Sciences, Rasht, Iran. Mohammad Hassan Hedayati Emami Department of Internal Medicine, Endocrinology and Metabolism Research Center, Guilan University of Medical Sciences, Rasht, Iran. Narges Alizadeh Department of Dermatology, Skin Research Center, Guilan University of Medical Sciences, Rasht, Iran. Rana Rafiei Department of Dermatology, Skin Research Center, Guilan University of Medical Sciences, Rasht, Iran. Hojat Eftekhari Department of Dermatology, Skin Research Center, Guilan University of Medical Sciences, Rasht, Iran. Kaveh Gharaei Nejad Department of Dermatology, Skin Research Center, Guilan University of Medical Sciences, Rasht, Iran. 2016 04 07 2017 02 01 Pemphigus vulgaris is a chronic autoimmune disease and glucocorticoids are one of the main treatments. Our study investigates the prevalence and associated factors of glucocorticoid-induced diabetes mellitus in these patients under different glucocorticoid regimens. 36 patients with first diagnosed Pemphigus vulgaris based on pathological and direct immunofluorescence findings who had received different glucocorticoid regimens (1-2 mg/kg oral or 1-2 mg/kg oral with 1g methylprednisolone pulse daily for 3 consecutive days with or without azathioprine) were evaluated during 2014-2016. Our study found that 22.2% of patients had impaired fasting glucose and incidence of corticosteroid-induced diabetes mellitus was 22.2% with no difference between oral and pulse therapy of corticosteroid. The first day after pulse therapy 19 patients of 21 had post bolus hyperglycemia that 36% of them became diabetic after 8 weeks. None of the variables, including age, BMI, HbA1c, LDL, HDL, TG, cholesterol, family history and blood pressure were associated with diabetes. Pretreatment FBS was the factor that would increase the likelihood of glucocorticoid-induced diabetes mellitus, 42.2% of patients with pretreatment FBS 100-126 developed diabetes in comparison with 17.2% in normal pretreatment FBS. Although the group who received azathioprine was associated with increased incidence of diabetes, the overall corticosteroid dose in this group was significantly higher than the other group (P=0.012), and controversy with other studies could be because of difference in corticosteroid dosage and small number of patients. The incidence of diabetes was not different between the group with glucocorticoid pulses and oral prednisolone without pulse therapy. Higher pretreatment FBS can be related to increased incidence of diabetes, but results from this study due to small number of patients are preliminary and multicenter studies are needed. https://acta.tums.ac.ir/index.php/acta/article/view/5561 https://acta.tums.ac.ir/index.php/acta/article/download/5561/4948

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 55 6 2017 08 19 381 388 EN Ata Pourabbasi Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran. Mehdi Tehrani-Doost Department of Psychiatry, Rouzbeh Hospital, Tehran University of Medical Sciences, Tehran, Iran. Soqra Ebrahimi Qavam Department of Educational Psychology, Faculty of Psychology and Education, Allameh Tabataba’ee University, Tehran, Iran. Jalal Farzami Department of Biostatistics, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran. Bagher Larijani Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran. 2017 01 31 2017 02 07 Diabetes in children and adolescents is a chronic condition with an expanding trend in the community. Several studies have shown cognitive dysfunctions are the most important side effects of diabetes among individuals of younger ages. Due to cultural differences and their impact on cognitive issues, the authors decided to assess the cognitive functions of Iranian children and adolescents with diabetes. Cognitive functions including memory, attention and executive functions were evaluated in 62 diabetic children and adolescents and healthy peers using CANTAB cognitive tests. Other data such as demographic, school performance and medical information were collected by questionnaires. Except in the case of few variables in RVP, SSP and SST, no significant difference exists between diabetic children and the control group in terms of different cognitive domains. But cognitive variables, especially in PRM, SWM and SOC test, has been shown to be deteriorated with increasing HbA1C values in serum levels. Diabetes has no impact on the cognitive functioning of children provided by maintaining a glycemic control. It is proposed that the adoption of appropriate parenting styles and family and social support can prevent cognitive changes in children with diabetes. https://acta.tums.ac.ir/index.php/acta/article/view/6197 https://acta.tums.ac.ir/index.php/acta/article/download/6197/4949

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 55 6 2017 08 19 389 394 EN Niloofar Shekoohi Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Mohammad Hassan Javanbakht Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Marjan Sohrabi Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Mahnaz Zarei Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Hamed Mohammadi Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Mahmoud Djalali Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. 2016 05 20 2017 02 01 Smoking may modify the appetite, and consequently affect nutrient intake and serum micronutrients. The effect of smoking on vitamin B12 status has been considered in several studies. The research proposed that organic nitrites, nitro oxide, cyanides, and isocyanides of cigarette smoke interfere with vitamin B12 metabolism, and convert it to inactive forms. This research was carried out to determine the serum level of active and inactive forms of vitamin B12 in male smokers in comparison with male nonsmokers. This is a case-control study, in which the participants were 85 male smokers and 85 male nonsmokers. The serum levels of total and active form of vitamin B12 were measured. Dietary intake was recorded by a quantitative food frequency questionnaire and one-day 24-hour dietary recall method. Independent two sample T test was used to compare quantitative variables between the case and control groups. The serum level of total vitamin B12 was not significantly different between two groups, but serum level of active form of vitamin B12 in the smoking group was significantly lower than non-smoking group (P<0.001). This is one of the first studies that evaluated the serum level of active form of vitamin B12 in smokers in the Iranian community. The results of this study identified that serum level of total vitamin B12 might be not different between smoking and non-smoking people, but the function of this vitamin is disturbed in the body of smokers through the reduction of serum level of active form of vitamin B12. https://acta.tums.ac.ir/index.php/acta/article/view/5648 https://acta.tums.ac.ir/index.php/acta/article/download/5648/4950

Tehran University of Medical Sciences Acta Medica Iranica 0044-6025 55 6 2017 08 19