HUMAN PREADIPOCYTES INHIBIT PROLIFERATION OF MCF-7 BREAST CANCER CELL LINE

Abstract

Cancer cell-stromal cell interaction plays a crucial role in the growth and invasion of tumor cells of various organs. It has been recently suggested that adipocytes and preadipocytes, two specific types of stromal cells, affect the biological behavior of variety of breast cancer cell lines. In spite of few investigations, there is a controversy in the literatures about the effects of adipocytes and preadipocytes on tumor growth. In this study, we compared the proliferation of MCF-7 breast-cancer cell line in culture with or without presence of primary isolated human adipocytes and preadipocytes by MTT colorimetric assay in 2, 4 and 6 days of culture experiments. Human adipocytes increased the proliferation of MCF-7 cells in the co-culture about 8.6%, 12% and 12% more than MCF-7 monoculture in 2, 4 and 6 days after cell culture respectively. Human preadipocytes decreased the proliferation of MCF-7 cells in the co-culture about 7.7%, 16.9% and 21% lesser than MCF-7 cell monoculture in 2, 4 and 6 days after cell culture, respectively. Proliferation of MCF-7 cells in co- culture with adipocytes were 17.7%, 35% and 42% more than MCF-7 cells in co-culture with preadipocytes after 2, 4 and 6 days of culture, respectively. We concluded that human adipocytes increase the proliferation of MCF-7 breast cancer cell line but human preadipocytes inhibit proliferation of MCF-7 breast cancer cell line.