Novel Biochemical Markers: Early Detection and Prevention of Malignant Transformation A Pilot Study

  • Abhishek Singh Nayyar Department of Oral Medicine and Radiology, Post-Graduate, Government Dental College and Research Institute, Bangalore-560002, Karnataka, India.
Keywords: Anti-oxidants, Carcinogenesis, Free radicals, Malignant transformation, Pre-cancerous, Reactive oxygen species


The role of oxygen free radicals in the initiation, promotion and progression of carcinogenesis and the protective role of anti-oxidant defenses has been the subject of much speculation in the recent past with conflicting reports in the literature. In recent years, increasing experimental and clinical data have provided compelling evidence for the involvement of oxidative stress in a large number of pathological states including cancers. The aim of this study was to measure the concentration of serum total proteins and albumin as potent anti-oxidants in the sera of patients diagnosed with speckled leukoplakia, one of the oral pre-cancerous lesions reported to have significantly high malignant transformation rates and well-differentiated oral squamous cell carcinoma. The study consisted of sera analysis of total protein and albumin levels in patients with speckled leukoplakia and histologically proven, well-differentiated oral squamous cell carcinoma. One way analyses of variance (ANOVA) was used to test the difference between groups. To find out which of the two groups' means were significantly different; post-hoc test of Scheffe was used. The study revealed variations in sera levels of albumin to be statistically significant. The results obtained emphasize the need for more studies with larger sample sizes to be conducted before a conclusive role for sera levels of total protein and albumin could be drawn as markers of transition from the various oral pre-cancerous lesions and conditions to frank oral squamous cell carcinoma.


Kolanjiappan K, Ramachandran CR, Manoharan S. Biochemical changes in tumor tissues of oral cancer patients. Clin Biochem 2003;36(1):61-5.

Hershkovich O, Shafat I, Nagler RM. Age-related changes in salivary antioxidant profile: possible implications for oral cancer. J Gerontol A Biol Sci Med Sci 2007;62(4):361-6.

Khanna R, Thapa PB, Khanna HD, Khanna S, Khanna AK, Shukla HS. Lipid peroxidation and antioxidant enzyme status in oral carcinoma patients. Kathmandu Univ Med J (KUMJ) 2005;3(4):334-9.

Bahar G, Feinmesser R, Shpitzer T, Popovtzer A, Nagler RM. Salivary analysis in oral cancer patients: DNA and protein oxidation, reactive nitrogen species, and antioxidant profile. Cancer 2007;109(1):54-9.

Demirbilek ME, Kilic N, Komurcu HF, Akin KO. Advanced oxidant protein products in aged with dementia. Am J Immunol 2007;3(2):52-5.

Iwao Y, Anraku M, Hiraike M, Kawai K, Nakajou K, Kai T, Suenaga A, Otagiri M. The structural and pharmacokinetic properties of oxidized human serum albumin, advanced oxidation protein products (AOPP). Drug Metab Pharmacokinet 2006;21(2):140-6.

Elango N, Samuel S, Chinnakkannu P. Enzymatic and non-enzymatic antioxidant status in stage (III) human oral squamous cell carcinoma and treated with radical radio therapy: influence of selenium supplementation. Clin Chim Acta 2006;373(1-2):92-8.

Ihara H, Hashizume N, Hasegawa T, Yoshida M. Antioxidant capacities of ascorbic acid, uric acid, alphatocopherol, and bilirubin can be measured in the presence of another antioxidant, serum albumin. J Clin Lab Anal 2004;18(1):45-9.

Barle H, Hammarqvist F, Westman B, Klaude M, Rooyackers O, Garlick PJ, Wernerman J. Synthesis rates of total liver protein and albumin are both increased in patients with an acute inflammatory response. Clin Sci (Lond) 2006;110(1):93-9.

Nicholson JP, Wolmarans MR, Park GR. The role of albumin in critical illness. Br J Anaesth 2000;85(4):599-610.

Zoellner H, Höfler M, Beckmann R, Hufnagl P, Vanyek E, Bielek E, Wojta J, Fabry A, Lockie S, Binder BR. Serum albumin is a specific inhibitor of apoptosis in human endothelial cells. J Cell Sci 1996;109 ( Pt 10):2571-80.

Kouoh F, Gressier B, Luyckx M, Brunet C, Dine T, Cazin M, Cazin JC. Antioxidant properties of albumin: effect on= oxidative metabolism of human neutrophil granulocytes. Farmaco 1999;54(10):695-9.

James WP, Hay AM. Albumin metabolism: effect of the nutritional state and the dietary protein intake. J Clin Invest 1968;47(9):1958-72.

Soejima A, Matsuzawa N, Hayashi T, Kimura R, Ootsuka T, Fukuoka K, Yamada A, Nagasawa T, Era S. Alteration of redox state of human serum albumin before and after hemodialysis. Blood Purif 2004;22(6):525-9.

Himmelfarb J, McMonagle E. Albumin is the major plasma protein target of oxidant stress in uremia. Kidney Int 2001;60(1):358-63.

How to Cite
Singh Nayyar A. Novel Biochemical Markers: Early Detection and Prevention of Malignant Transformation A Pilot Study. Acta Med Iran. 50(9):597-602.