Interleukin 17 receptor gene polymorphism in periimplantitis and chronic periodontitis.

  • Mahdi Kadkhodazadeh Department of Periodontics, Dental Research Center, Dental School, Shahid Beheshti Medical University, Tehran, Iran.
  • Ahmad Reza Ebadian Dentist, Private Practitioner, Mashhad, Iran
  • Reza Amid Department of Periodontics, Dental School, Shahid Beheshti Medical University, Evin, Tehran, Iran.
  • Navid Youssefi Shahid Beheshti Medical University, Tehran, Iran.
  • Amir Reza Mehdizadeh Shahid Beheshti Medical University, Tehran, Iran.
Keywords: IL-17R, IL-17, Periodontitis, Periimplantitis, Polymorphism

Abstract

Gene polymorphism of cytokines influencing their function has been known as a contributing factor in the pathogenesis of inflammatory diseases of the tooth and implant supporting tissues. The aim of this study was to investigate the association of IL-17R gene polymorphism (rs879576) with chronic periodontitis and periimplantitis in an Iranian population. 73 patients with chronic periodontitis, 37 patients with periimplantitis and 83 periodontally healthy patients were enrolled in this study. 5cc blood was obtained from each subject's arm vein and transferred to tubes containing EDTA. Genomic DNA was extracted using Miller's Salting Out technique. The DNA was transferred into 96 division plates, transported to Kbioscience Institute in United Kingdom and analyzed using the Kbioscience Competitive Allele Specific PCR (KASP) technique. Chi-square and Kruskal Wallis tests were used to analyze differences in the expression of genotypes and frequency of alleles in disease and control groups (P-Value less than 0.05 was considered statistically significant). There were no significant differences between periodontitis, periimplantitis with AA, GG, GA genotype of IL-17R gene (P=0.8239). Also comparison of frequency of alleles in SNP rs879576 of IL-17R gene between the chronic periodontitis group and periimplantitis group did not revealed statistically significant differences (P=0.8239). The enigma of IL-17 and its polymorphism-role in periodontitis and periimplantitis is yet to be investigated more carefully throughout further research but this article demonstrates that polymorphism of IL-17R plays no significant role in incidence of chronic periodontitis and Periimplantitis.

References

Sakka S, Coulthard P. Implant failure: etiology and complications. Med Oral Patol Oral Cir Bucal 2011;16(1):e42-4.

Mombelli A, Lang NP. The diagnosis and treatment of peri-implantitis. Periodontol 2000 1998;17:63-76.

Bormann KH, Stühmer C, Z'Graggen M, Kokemöller H, Rücker M, Gellrich NC. IL-1 polymorphism and periimplantitis. A literature review. Schweiz Monatsschr Zahnmed 2010;120(6):510-20.

Ata-Ali J, Candel-Marti ME, Flichy-Fernández AJ,m Peñarrocha-Oltra D, Balaguer-Martinez JF, Peñarrocha Diago M . Peri-implantitis: associated microbiota and treatment. Med Oral Patol Oral Cir Bucal 2011;16(7):e937-43.

Roos-Jansa ker AM, Lindahl C, Renvert H, Renvert S. Nine- to fourteen-year follow up of implant treatment: I. Implant loss and associations to various factors. J ClinPeriodontol 2006;33(4):283-9.

Schwartz-Arad D, Laviv A, Levin L. Failure causes, timing, and cluster behavior: an 8-year study of dental implants. Implant Dent 2008;17(2):200-7.

Zitzmann NU, Berglundh T. Definition and prevalence of peri-implant diseases. J Clin Periodontol 2008;35(8 Suppl):286-91.

Michalowiz B S, Diehl S R, Gunsolley J C, Sparks, B S, Brooks C N, Koertge T E, Califano J V, Burmeister J A, Schenkein H A. Evidence of a substantial genetic basis for risk of adult periodontitis. J Periodontol 2000;71(11):1699-707.

Woo P. Cytokine polymorphisms and inflammation. Clin Exp Rheumatol 2000;18(6):767–71.

Candel-Martí ME, Flichy-Fernández AJ, Alegre-Domingo T, Ata-Ali J, Peñarrocha-Diago MA. Interleukins IL-6, IL- 8, IL-10, IL-12 and periimplant disease. An update. Med Oral Patol Oral Cir Bucal 2011;16(4):e518-21.

Yao Z, Spriggs MK, Derry JM, Strockbine L, Park LS, VandenBos T, Zappone JD, Painter SL, Armitage RJ. Molecular characterization of the human interleukin (IL)- 17 receptor. Cytokine 1997;9(11):794-800.

Pradeep AR, Hadge P, Chowdhry S, Patel S, Happy D. Exploring the role of Th1 cytokines: interleukin-17 and interleukin-18 in periodontal health and disease. J Oral Sci 2009;51(2):261-6.

Takahashi K, Azuma T, Motohira H, Kinane DF, Kitetsu S. The potential role of interleukin-17 in the immunopathology of periodontal disease. J Clin Periodontol 2005;32(4):369-74.

Wagner J, Kaminski WE, Aslanidis C, Moder D, Hiller KA, Christgau M, Schmitz G, Schmalz G. Prevalence of OPG & IL-1 gene polymorphisms in chronic periodontitis.J Clin Periodontol 2007;34(10):823-7.

Qyirynen M, De Soete M, Van Steenberghe D. Infectious risks for oral implants. Clin Oral Implant Res 2002;13(1):1-19.

Kadkhodazadeh M, Amid R. Evaluation of peri-implant tissue health using a scoring system. JIACD 2012;4:51-7.

Severino VO, Napimoga MH, de Lima Pereira SA. Expression of IL-6, IL-10, IL-17 and IL-8 in the periimplant crevicular fluid of patients with peri-implantitis.Arch Oral Biol 2011;56(8):823-8.

Ozçaka O, Nalbantsoy A, Buduneli N.Interleukin-17 and interleukin-18 levels in saliva and plasma of patients with chronic periodontitis. J Periodontal Res 2011;46(5):592-8.

Pradeep AR, Hadge P, Chowdhry S, Patel S, Happy D. Exploring the role of Th1 cytokines: interleukin-17 and interleukin-18 in periodontal health and disease. Oral Sci 2009;51(2):261-6.

Fumagalli M, Pozzoli U, Cagliani R, Comi GP, Riva S, Clerici M, Bresolin N, Sironi M. Parasites represent a major selective force for interleukin genes and shape the genetic predisposition to autoimmune conditions. J Exp Med 2009;206(6):1395-408.

Pu X, Ye Y, Spitz MR, Wang L, Gu J, Lippman SM, Hildebrandt MAT, Hong WK, Minna JD, Roth JA, Yang P, Wu X . Predictors of survival in never-smokers with non-small cell lung cancer: A large-scale, two-phase genetic study. Clin Cancer Res 2012;18(21):5983-91.

Yu JJ, Ruddy MJ, Conti HR, Boonanantanasarn K, Gaffen SL. The interleukin-17 receptor plays a gender-dependent role in host protection against Porphyromonas gingivalisinduced periodontal bone loss. Infect Immun 2008;76(9):4206-13.

How to Cite
1.
Kadkhodazadeh M, Ebadian AR, Amid R, Youssefi N, Mehdizadeh AR. Interleukin 17 receptor gene polymorphism in periimplantitis and chronic periodontitis. Acta Med Iran. 51(6):353-358.
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