Detection of Metallo-β-Lactamase Producing Pseudomonas aeruginosa Isolated from Public and Private Hospitals in Baghdad, Iraq
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Abstract
Metallo-β-lactamase (MBL) producing Pseudomonas aeruginosa has been reported to be an important nosocomial infection. Its intrinsic and acquired resistance to various antimicrobial agents and its ability to develop multidrug resistance imposes a serious therapeutic problem. Different clinical samples were collected from public and private hospitals in Baghdad city, Iraq. Bacterial identification was done using conventional cultural, biochemical tests, and VITEk 2 system. Minimum inhibitory concentration (MIC) testing was performed using VITEK 2 automated system. Each P. aeruginosa isolates showed resistance to Carbapenems (Imipenem and Meropenem) were subjected to Imipenem-EDTA combined disc synergy test (CDST) to investigate the production of MBL (confirmative test). The presence of bla-genes encoded IMP, VIM, and SPM-1 was detected by conventional PCR technique. A total of 75 P. aeruginosa isolates were isolated, 16 (21.3%) were able to grow on MacConkey agar supplemented with Meropenem 4mg/L (MMAC). The MIC of different antibiotics showed that 6 (37.5 %) isolates were Carbapenem resistant, MIC ≥16 µg/ml while 4 (25%) isolates appear to be MBL producer using CDST test. PCR assay revealed that 3 (50%), 1 (16.6%) of the carbapenem resistant isolates harbored blaIMP, blaSPM-1 genes, respectively. blaVIM gene was not detected in this study. The prevalence of multi-drug resistant P. aeruginosa isolates especially Carbapenem resistant bacteria was increased in Baghdad province. The blaIMP was the predominant among the MBLs genes in P. aeruginosa in this study.
Murray PR, Baron EJ, Jorgensen JH, et al, editors. Manual of Clinical Microbiology. 8th ed. Washington, D.C: American Society for Microbiology Press; 2003: p. 719-28.
Saderi H, Karimi Z, Owlia P, et al. Phenotypic Detection of Metallo-β-lactamase Producing Pseudomonas aeruginosa Strains Isolated from Burned Patients. Iran J Pathol 2008;17(3):20-4.
Livermore DM, Woodford N. Carbapenemases: a problem in waiting? Curr Opin Microbiol 2000;3(5):489-95.
Pitout JD, Gregson DB, Poirel L, et al. Detection of Pseudomonas aeruginosa Producing Metallo-β- Lactamases in a Large Centralized Laboratory. J Clin Microbiol 2005;43(7):3129-35.
Kaleem F, Usman J, Hassan A, et al. Frequency and susceptibility pattern of metallo-beta-lactamase producers in a hospital in Pakistan. J Infect Dev Ctries 2010;4(12):810-3.
Pournaras S, Maniati M, Petinaki E, et al. Hospital outbreak of multiple clones of Pseudomonas aeruginosa carrying the unrelated metallo-β-Lactamase gene variants blaVIM-2 and blaVIM-4. J Antimicrob Chemother 2003;51(6):1409-14.
Poirel L, Lambert T, Türkoglü S, et al. Characterization of class 1 integrons from Pseudomonas aeruginosa that contain the blaVIM-2 carbapenem-hydrolyzing β- lactamase gene and of two novel aminoglycoside resistance gene cassettes. Antimicrob Agents Chemother 2001;45(2):546-52.
Rossolini GM, Mantengoli E. Treatment and control of severe infections caused by multiresistant Pseudomonas aeruginosa. Clin Microbiol Infect 2005;11(Suppl 4):17-32.
Bergès L, Rodriguez-Villalobos H, Deplano A, et al. Prospective evaluation of imipenem/EDTA combined disc and E-test for detection of metallo-beta-lactamase producing Pseudomonas aeruginosa. J Antimicrob Chemother 2007;59(4):812-3.
Lee, K, Lim YS, Yong D, et al. Evaluation of the Hodge test and the imipenem-EDTA double disk synergy test for differentiating Metallo-β-Lactamase producing isolate of Pseudomonas spp. and Acinetobacter spp. J Clin Microbiol 2003; 41(10):4623-9 .
Performance Standards for Antimicrobial Susceptibility Testing. 22nd Informational Supplement. (Accessed in May 2015, 1, at http://antimicrobianos.com.ar/ATB/wpcontent/ uploads/2012/11/M100S22E.pdf.(
Sambrook J, Russell DW, editors. Molecular Cloning: A Laboratory Manual. 3rd. ed. NY, USA: Cold Spring Harbor Laboratory Press; 2001: p.
Khosravi AD, Mihani F. Detection of metallo-β-lactamase producing Pseudomonas aeruginosa strains isolated from burn patients in Ahwaz, Iran. Diagn Microbiol Infect Dis 2008;60(1):125-8.
Cedric M, John P, Ivan R, et al, editors. Hospital infection, Sterilization and Disinfection. Medical Microbiology, London: Mosby Publisher; 1998: p. 481.
Timothy RW, Mark AT, Laurent P, et al. Metallo-β- lactamase a quite before storm. Clin Microbiol Rev 2005;18(2):306-25.
Sacha P, Żórawski M, Hauschild T, et al. The presence ofblaIMP genes on plasmids DNA isolated from multidrugresistant Pseudomonas aeruginosa strains at University Hospital in Bialystok (Poland) - first report. Folia Histochemica et Cytobiologica 2007;45(4):405-8.
Varsha G. Metallo-β-lactamases in Pseudomonas aeruginosa and Acinetobacter species. Expert Opin Investig Drugs 2008;17(2):131-43.
Wydmuch Z, Ciolek O, Cholewa K, et al. GyrAmutations in ciprofloxacin resistant clinical isolates of Pseudomonas aeruginosa in Silesian Hospital in Poland. Polish J Microbiol 2005;54(3):201-6.
Tsakris A, Pournaras S, Woodford N, et al. Outbreak of infections caused by Pseudomonas aeruginosa producing VIM-1 carbapenemase in Greece. J Clin Microbiol 2000;38(3):1290-2.
Rossi F. The Challenges of Antimicrobial Resistance in Brazil. Clin Infect Dis 2011;52(9):1138-43.
Murphy TA, Simm AM, Toleman MA, et al. Biochemical Characterization of the Acquired Metallo-β-lactamase SPM-1 from Pseudomonas aeruginosa. Antimicrob Agents Chemother 2003;47(2):582-7.
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| Issue | Vol 54, No 2 (2016) | |
| Section | Original Articles | |
| Keywords | ||
| Pseudomonas aeruginosa MBL Carbapenems ESBL CRPA Bla genes | ||
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