Acta Medica Iranica 2016. 54(4):240-244.

Attenuated Lead Induced Apoptosis in Rat Hepatocytes in the Presence of Lycopersicon Esculentum
Hamidreza Ahmadi Ashtiani, Arash Khaki, Shahram Ejtemaei Mehr, Soghra Anjarani, Manochehr Dadgarnejad, Mahmoud Alebouyeh, Hossein Rastegar

Abstract


Lead (Pb), has, for decades, being known for its adverse effects on various body organs and systems. In the present study, the damage of Pb on the Liver tissue apoptosis was investigated, and Lycopersicon esculentum as an antioxidants source was administered orally to prevent the adverse effects of Pb. Eighteen Wistar rats, randomized into three groups (n=6), were used for this study. Animals in Group A served as the control and were drinking distilled water. Animals in Groups B and C were drinking 1%Lead acetate (LA). Group C animals were, in addition to drinking LA, treated with 1.5 ml/day of Lycopersicon esculentum. Treatments were for three months. The obtained results showed that lead acetate caused significant reductions in the liver weight, plasma and tissue superoxide dismutase and catalase activity, but a significant increase in plasma and tissue malondialdehyde concentration but Lycopersicon esculentum have an inhibitory effect on LA liver adverse effect. So, it can be concluded that Lycopersicon esculentum have a significant protective effect on liver lead acetate adverse effects as well as, lead acetate -induced oxidative stress.


Keywords


Lead; Apoptosis; Hepatocytes; Lycopersicon

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References


Gidlow DA. Lead toxicity. Occup Med (Lond) 2004;54(2):76-81.

Manesh FK, Erler JT, Gonick HC. Experimental model of lead nephropathy: Correlation between renal functional changes and hematological indices of lead toxicity. J Trace Elem Electrolytes Health Dis 1994;8(1):9-13.

Waldron HA. The Anaemia of Lead Poisoning: A Review. Br J Ind Med 1966;23(2):83-100.

Hopkins A. Experimental lead poisoning in the baboon. Br J Ind Med 1970;27(2):130-40.

Patocka J, Cerný K. Inorganic lead toxicology. Acta Medica (Hradec Kralove) 2003;46(2):65-72.

Gurer-Orhan H, Sabir HU, Ozgüneş H. Correlation between clinical indicators of lead poisoning and oxidative stress parameters in controls and lead-exposed workers. Toxicology 2004;195(2-3):147-54.

Lisa T. The Top 10 Antioxidant Foods. Back to nutrition archives, Better Nutrition, 2002. (Accessed in May 12, 2015, at http://naturefirstusa.org/Special%20Reports%5 Cantioxidants%5CThe%20Top%2010%20Antioxidant%20Foods.htm).

Jeanie LD. Tomato and Broccoli-2 Antioxidant Power Blasts, 2007. (Accessed in May 16, 2015, at http://www. webmed.com/food-recipes/tasty-tomato-antioxi dant- power-blast).

Nguyen ML, Schwartz SJ. Lycopene: chemical and biological properties. Food Technol 1999;53(2):38-45.

Shi J, Le Maguer M. Lycopene in Tomatoes: Chemical and Physical Properties Affected by Food Processing. Crit Rev Biotechnol 2000;20(4):293-334.

Sandhu JS, Krasnyanski SF, Domier LL, et al. Oral immunization of mice with transgenic tomato fruit expressing respiratory syncytial virus-F protein induces a systemic immune response. Transgenic Res 2000;9(2):127-35.

Rajaii F, Khaki AA, Khaki A, et al. Histopathological effect of Sulfur Dioxide in Mouse Liver Following the Chronic and Acute Exposure. Pakistan J Biol Sci 2008;8:1241-5.

Khaki A. Protective effect of quercetin against necrosis and apoptosis induced by experimental ischemia and reperfusion in rat liver. African J Pharmacy Pharmacol 2010;4(1):22-6.

Hodgson E, editor. A textbook of modern toxicology. 3rd ed. Hoboken, N.J: Wiley-Interscience; 2004.

Auman JT, Chou J, Gerrish K, et al. Identification of genes implicated in methapyrilene-induced hepatotoxicity by comparing differential gene expression in target and nontarget tissue. Environ Health Perspect 2007;115(4):572-78.

Waters M, Stasiewicz S, Merrick BA, et al. CEBS – Chemical Effects in Biological Systems: a public data repository integrating study design and toxicity data with microarray and proteomics data. Nucleic Acids Res 2008;36(Database issue):D892-900.

Pande M, Flora SJ. Lead induced oxidative damage and its response to combined administration of lipoic acid and succimers in rats. Toxicology 2002;177(2-3):187-96.

Upasani CD, Khera A, Balaraman R. Effect of lead with Vitamins E, C, or spirulina on malondialdehyde: conjugated dienes and hydroperoxides in rats. Indian J Exp Biol 2001;39(1):70-4.

Adanaylo V, Oteiza P. Lead intoxication: antioxidant defence and oxidative stress in rat brain. Toxicol 1999;135:77-85.

Sandhir R, Gill KD. Effect of lead on lipid peroxidation in liver of rats. Biol Trace Elem Res 1995;48(1):91-7.

Gurer H, Ercal N. Can antioxidant be beneficial in the treatment of lead poisoning? Free Radic Biol Med 2000;29(10):927-45.

Flora SJ, Tandon SK. Preventive and therapeutic effects of thiamine, ascorbic acid and their combination in lead intoxication. Acta Pharmacol Toxicol 1986;58(5):374-8.

Patra RC, Swarup D. Effect of antioxidant ascorbic acid, L-methionine on tocopherol alone or along with chelator on cardiac tissue of lead-treated rats. Vet Arch 2004;74(3):235-44.

Pinon-Lataillade G, Thoreux-Manlay A, Coffigny H, et al. Reproductive toxicity of chronic lead exposure in male and female mice. Hum Exp Toxicol 1995;14(11):872-8.

Wang C, Zhang Y, Liang J, et al. 'Impacts of ascorbic acid and thiamine supplementation at different concentrations on lead toxicity in testis. Clin Chim Acta 2006;370(1-2):82-8.

Li JJ, Tang Q, Li Y, et al. Role of oxidative stress in the apoptosis of hepatocellular carcinoma induced by combination of arsenic trioxide and ascorbic acid. Acta Pharmacol Sin 2006;27(8):1078-84.

Yu X, Kubota H, Wang R, et al. Involvement of Bcl-2 family genes and fas signaling systemin primary and secondary male germ cell apoptosis induced by 2-bromopropane in rat. Toxicol Appl Pharmacol 2001;174(1):35-48.


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