The Role of TNF-α in Aflatoxin B-1 Induced Hepatic Toxicity in Isolated Perfused Rat Liver Model

  • Kazem Koohi Mohammad Department of Basic Sciences, School of Veterinary Medicine, Tehran University, Tehran, Iran.
  • Mahmoud Ghazi-Khansari Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Farzad Hayati Department of Surgery and Radiology, School of Veterinary Medicine, University of Tehran, Tehran, Iran.
  • Hamid Staji Department of Pathobiology, School of Veterinary Medicine, Semnan University, Semnan, Iran.
  • Morteza Keywanloo Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Semnan University, Semnan, Iran.
  • Ebrahim Shahroozian Department of Basic Sciences, School of Veterinary Medicine, Semnan University, Semnan, Iran.
Keywords: Aflatoxin B1, TNF-α, IPRL

Abstract

Aflatoxin B-1 (AFB1) is one of the major mycotoxins causing food contamination. Previous studies have shown that AFB1 can induce carcinogenicity and toxic effects in the isolated perfused rat liver and these effects are associated with its metabolites and peroxidation activity. Here we surveyed whether these pathogenic effects of AFB1 are associated with TNF-α as an inflammatory cytokine in general liver damages. In this study, we used twenty male Wistar rats (250-300 g). Rats were divided into four groups. Control group was pre-treated with LPS and then perfused with KHBB. The second group was pretreated with PTX and LPS and then perfused with KHB. The third group was pre-treated with LPS and then perfused with AFB-1 and KHB. The last group was pretreated with LPS and PTX and then perfused with AFB1 and KHB. Results revealed that aflatoxin B1 significantly increased the enzyme activity of aminotransferase and levels of lipid peroxidation. Also, the levels of Glutathione decreased in the aflatoxin group significantly. TNF-α released in perfusate and increased in aflatoxin B1 group significantly and decreased in AFB-1+PTX. Exposure to Aflatoxin B1 may induce reactive oxygen species, so these species may induce overproduction of proinflammatory cytokines such as TNF-α and may cause more damage to hepatic cells.

Author Biography

Ebrahim Shahroozian, Department of Basic Sciences, School of Veterinary Medicine, Semnan University, Semnan, Iran.
Department

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Published
2017-09-16
How to Cite
1.
Koohi Mohammad K, Ghazi-Khansari M, Hayati F, Staji H, Keywanloo M, Shahroozian E. The Role of TNF-α in Aflatoxin B-1 Induced Hepatic Toxicity in Isolated Perfused Rat Liver Model. Acta Med Iran. 55(7):416-421.
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Original Article(s)