Acta Medica Iranica 2017. 55(10):616-620.

Study of Serum Malondialdehyde Level in Opioid and Methamphetamine Dependent Patients
Khadije Najafi, Sajad Ahmadi, Mahdi Rahpeyma, Habibolah Khazaie, Asad Vaisi-Raygani, Ali Moini, Amir Kiani

Abstract


Opioid compound and methamphetamine are commonly used in drug abuse; these can disrupt the normal function of cellular and molecular systems, leading to several events such as oxidative stress, aging, apoptosis, and necrosis. Malondialdehyde (MDA) is the most important biomarker for evaluation of oxidative stress and determination of lipid peroxidation. In this study, 42 drug abusers and 22 healthy persons participated as case and control groups, respectively. MDA in volunteer sera was determined by high-performance liquid chromatography (HPLC) with fluorescence detection after pre-column derivatization using thiobarbituric acid. The analysis was performed on a ODS column by spectrofluorometer detection, operated at excitation of 515 nm and emission of 535 nm. A mixture of phosphate buffer (0.05 M, pH 6.8), containing potassium monobasic phosphate and methanol (60:40, v/v) at a flow rate of 1 ml/min, was used as the mobile phase. The retention time of MDA-TBA was 3.2 min. Our findings showed that the MDA level increased in the opioid and methamphetamine abusers when compared to the control group (P<0.05); however, no significant difference was observed between the opioid and methamphetamine groups. A state of oxidative stress during biological processes leads to lipid peroxidation, DNA damage, biomolecule dysfunctions, and many other diseases. Since it is impossible to eradicate the drug addiction, we should reduce the side effects of drug abuse, such as oxidative stress, by intake of proper nutrition and antioxidants.


Keywords


Opioid; Methamphetamine; Oxidative stress; Malondialdehyde

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References


Kobeissy F, Mouhieddine TH, Nokkari A, Itani M, Mouhieddine M, Zhang Z, et al. Recent updates on drug abuse analyzed by neuroproteomics studies: Cocaein, Methamphetamine and MDMA. Translation alproteomics.2014;3:38–52.

Cunha-Oliveira T, Rego A.C, Oliveira CR. Oxidative Stress and Drugs of Abuse: An Update. Mini-Reviews in Organic Chemistry.2013;10.

Nakashima k. High-Performance Liquid Chromatographic Analysis of Drug of Abuse in Biologic Sampels. Journal of health sciences.2005;51:272-277.

Da Costa JL, Chasin AA. Determination of MDMA, MDEA and MDA in urine by high performance liquid chromatography with fluorescence detection. J.Chromatog.2004;811:41–45.

Shahouzehi B, Shokoohi M, Najafipour H. The effect of opium addiction on serum adiponectin and leptin levels in male subjects: a case control study from Kerman coronary artery disease risk factors study (kercadrs).EXCLI Journal.2013;12:916-923.

Macotpet A, Suksawat F, Sukon P, Pimpakdee K, Pattarapanwichien E, Tangrassameeprasert R, et al. Oxidative stress in cancer-bearing dogs assessed by measuring serum malondialdehyde. BMC Veterinary Research.2013;9.

Rahman T, Hosen I, Islam M.M.T, Uddin Shekhar H. Oxidative stress and human health. Advances in Bioscience and Biotechnology.2012;3:997-1019.

Yamamoto BK, Bankson MG. Amphetamine neurotoxicity: cause and consequence of oxidative stress. Crit Rev. Neurobiol.2005;17:87-117.

Cadet JL, Krasnova IN, Jayanthi S, Lyles J. Neurotoxicity of substituted amphetamines: molecular and cellular mechanisms. Neurotox.Res.2007;11:183-202.

Fitzmaurice PS, Tong J, Yazdanpanah M, Liu PP, Kalasinsky KS, Kish SJ. Levels of 4-hydroxynonenal and malondialdehyde are increased in brain of human chronic users of methamphetamine. J.Pharmacol. Exp.Ther.2006;319:703-709.

Shin EJ, Duong CX, Nguyen XK, Li Z, Bing G, Bach JH, et al. Role of oxidative stress in methamphetamine-induced dopaminergic toxicity mediated by protein kinase C delta. Behav.Brain Res.2012;232:98-113.

Ozmen I, Naziroglu M, Alici H.A, Sahin F, Cengiz M, Eren I. Spinal morphine administration reduces the fatty acid contents in spinal cord and brain by increasing oxidative stress. Neurochem. Res.2007;32:19-25.

Güney Y, Bilgihan A, Ulukavak Ciftçi T, Çimen F, Coskun O. serum malondialdehyde levels and superoxide dismutase activities in pulmonary tuberculosis and lung cancers. Meslek Yüksekokulu Dergisi.2004;6:33-38.

Serafin Mun˜oz1 A, Preciado Puga M, Wrobel K, Garay Sevilla ME, Wrobel K. Micro Assay for Malondialdehyde in Human Serum by Extraction-Spectrophotometry Using an Internal Standard. Microchim. Acta.2004;148:285–291.

DR S, Kumaran S, Annam V, Hamsaveena. Age related changes in malondialdehyde: total antioxidant capacity ratio – a novel marker of oxidative stress. International Journal of Pharma and Bio Sciences.2010;1.

Akande AA, Akinyinka AO. Serum malondialdehyde levels during menstral cycle. African Journal of Biotechnology.2005;4:1297-1299.

Atici S, Cinel I, Cinel L, Doruk N, Eskandari G, Oral U. Liver and kidney toxicity in chronic use of opioids: An experimental long term treatment model. J. Biosci.2005;30:245–252.

Othman GQ, Amin YK. Oxidative Stress and Some Cellular Blood Variables in Morrphine Addicted Female Rats. Tikrit Medical Journal.2012;18:134-142.

Lord KC, Shenouda SK, McIlwain E, Charalampidis D, Lucchesi PA, Varner KJ. Oxidative stress contributes to methamphetamine- induced left ventricular dysfunction. Cardiovasc Res.2010;87:111-118.

Yamamoto BK, Zhu W. The Effects of Methamphetamine on the Production of Free

Radicals and Oxidative Stress. JPET.1998;287:107–114.

Karatas F, Karatepe M, baysar A.determination of free malondialdehyde in human serum by high-performance liquid chromatography. Analytical biochemistery.2002;311:76-79.


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