Evaluation of Genistein Effect on Invasion of Breast Cancer Stem Cell-Like Cells

  • Farnaz Malekmarzban Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Toxicology and Pharmacology, Poisoning Research Center, Tehran University of Medical Sciences, Tehran, Iran.
  • Vahideh Montazeri Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Toxicology and Pharmacology, Poisoning Research Center, Tehran University of Medical Sciences, Tehran, Iran.
  • Maryam Majdzadeh Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Toxicology and Pharmacology, Poisoning Research Center, Tehran University of Medical Sciences, Tehran, Iran.
  • Seyed Nasser Ostad Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Toxicology and Pharmacology, Poisoning Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Keywords: MDA-MB-231, CD44 /CD24-cells, Cytotoxicity, Genistein

Abstract

It is established that cancer stem cells (CSCs) have potential in tumor formation, progression, and metastasis in different types of solid tumors. Additionally, recent studies have demonstrated that Genistein consumption is correlated with lowered cancer rate and metastasis decrease. In this study, we premised that Genistein could be an influential agent targeting breast cancer stem cell-like cells. In this survey, CD44+/CD24-cells (cancer stem cell-like cells) were isolated from MDA-MB-231 cells by magnet-activated cell sorting (MACS). The effect of various concentrations of Genistein on the cell proliferation and viability in MDA-MB-231 CD44+/CD24-cells tested in comparison to their parental cells utilizing MTT assay, invasion assay. The results of this study show that Genistein could inhibit invasion in MDA-MB-231 cells. Also, we observed that Genistein could be efficient for invasion inhibition in CD44+/CD24-cells. Considering the finding that Genistein could have desired effects on MDA-MB-231 as well as CD44+/CD24-cells, we demonstrated that Genistein could be an influential agent for treating malignant and invasive breast tumors.

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Published
2018-06-23
How to Cite
1.
Malekmarzban F, Montazeri V, Majdzadeh M, Ostad SN. Evaluation of Genistein Effect on Invasion of Breast Cancer Stem Cell-Like Cells. Acta Med Iran. 56(6):360-365.
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Articles