Prevalence and Serotype Determination of Streptococcus agalactiae Isolated From Non-Pregnant Women in Tehran, Iran
Abstract
Consequence of Streptococcus agalactiae, Group B Streptococcus (GBS) relating infant’s diseases are well documented. Although many women carry this bacterium in their vagina, they may transfer to their infant during delivery and may result in different neonatal invasive diseases. The aim of this study was to determine the prevalence of GBS and serotyping the isolated species among un-selective non-pregnant women who attended two gynecology clinics in Tehran. In this cross-sectional study, a total of 560 vaginal samples collected from non-pregnant women. Following inoculation of the specimen on Blood Agar, the standard technology was applied for the final identification of GBS. Detected GBS species were further confirmed using specific PCR directed on dlts gene. Capsular serotyping was done by using the multiplex polymerase chain reaction (PCR) method. The chi-square method was used for statistical analysis. Fifty (8.9%) out of 560 non-pregnant women were carriers of GBS. The most common types were III (36%), followed by type II (32%), Ia (26%), and Ib (6%), respectively. Results represent that the prevalence rate of GBS in non-pregnant women was reliable and similar to what obtained from pregnant women. In addition, the serotype III was found the most dominant types, as well as other investigations in the Tehran area. Therefore, vaccine designation based on type III is recommended.
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19. Sharmila V, Joseph NM, Babu TA, Chaturvedula L, Sistla S. Genital tract group B streptococcal colonization in pregnant women: a South Indian perspective. The Journal of Infection in Developing Countries. 2011;5(08):592-5.
20. Beigverdi R, Jabalameli F, Mirsalehian A, Hantoushzadeh S, Boroumandi S, Taherikalani M, et al. Virulence factors, antimicrobial susceptibility and molecular characterization of Streptococcus agalactiae isolated from pregnant women. Acta microbiologica et immunologica Hungarica. 2014;61(4):425-34. https://doi.org/10.1556/AMicr.61.2014.4.4.
21. Nahaei MR, Ghandchilar N, Bilan N, Ghahramani P. Maternal carriage and neonatal colonization of Streptococcus agalactiae in Tabriz, Northwest Iran. Iranian Journal of Medical Sciences. 2015;32(3):177-81.
22. Mousavi SM, Nassaj M, Arabestani MR, Nazeri H, Rahmaninan M, Hoseinzadeh H. Genotyping of Clinical Streptococcus agalactiae Strains Based on Molecular Serotype of Capsular (cps) Gene Cluster Sequences Using PCR Assay in Hamadan during 2013-2014. Infection, Epidemiology and Microbiology. 2016;2(4):12-6. URL: http://journals.modares.ac.ir/article-4-7183-en.html.
23. Kong F, Ma L, Gilbert GL. Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization. Journal of medical microbiology. 2005;54(12):1133-8. DOI: 10.1099/jmm.0.46244-0.
24. Eren A, Kucukercan M, Oguzoglu N, Unal N, Karateke A. The carriage of group B streptococci in Turkish pregnant women and its transmission rate in newborns and serotype distribution. Turk J Pediatr. 2005;47(1):28-33.
25. Ippolito DL, James WA, Tinnemore D, Huang RR, Dehart MJ, Williams J, et al. Group B streptococcus serotype prevalence in reproductive-age women at a tertiary care military medical center relative to global serotype distribution. BMC infectious diseases. 2010;10(1):336. doi: 10.1186/1471-2334-10-336.
2. Martins E, Andreu A, Melo-Cristino J, Ramirez M. Distribution of pilus islands in Streptococcus agalactiae that cause human infections: insights into evolution and implication for vaccine development. Clin Vaccine Immunol. 2013;20(2):313-6. doi: 10.1128/CVI.00529-12
3. Najarian N, Khalili MB, Astani A, Vakili M, Sadeh M. Serotype Determination of Streptococcus agalactiae Detected from Vagina and Urine of Pregnant Women in Yazd, Iran-2015. International Journal of Medical Laboratory. 2018;5(1):49-57. URL: http://ijml.ssu.ac.ir/article-1-228-en.html.
4. Nazari A, Khalili MB, Astani A, Vakili M, Sadeh M, Mojibiyan M, et al. Determination of Genotypes of Streptococcus agalactiae Isolated from both Urine and Vagina of Pregnant Women Referred to Gynecology Clinics of Yazd, Iran-2015. URL: http://ijml.ssu.ac.ir/article-1-169-en.html.
5. Poyart C, Tazi A, Réglier-Poupet H, Billoët A, Tavares N, Raymond J, et al. Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci. Journal of clinical microbiology. 2007;45(6):1985-8. doi: 10.1128/JCM.00159-07
6. Imperi M, Pataracchia M, Alfarone G, Baldassarri L, Orefici G, Creti R. A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae. Journal of microbiological methods. 2010;80(2):212-4. doi: 10.1016/j.mimet.2009.11.010.
7. Emaneini M, Jabalameli F, van Leeuwen WB, Beigverdi R. Prevalence of Group B Streptococcus in Pregnant Women in Iran. The Pediatric infectious disease journal. 2018;37(2):186-90.
8. Sadeh M, Firouzi R, Derakhshandeh A, Khalili MB, Kong F, Kudinha T. Molecular characterization of Streptococcus agalactiae isolates from pregnant and non-pregnant women at Yazd University Hospital, Iran. Jundishapur journal of microbiology. 2016;9(2). DOI: 10.5812/jjm.30412.
9. Hadavand S, Ghafoorimehr F, Rajabi L, Davati A, Zafarghandi N. Frequency of group B Streptococcal colonization in pregnant women aged 35-37 weeks in clinical centers of Shahed University, Tehran, Iran. Iranian journal of pathology. 2015;10(2):120. DOI: 10.7508/IJP.2015.02.006.
10. Fatemi F, Chamani L, Pakzad P, Zeraati H, Rabbani H, Asgari S. Colonization rate of group B Streptococcus (GBS) in pregnant women using GBS agar medium. Acta Medica Iranica. 2009;47(1):25-30.
11. Aali BS. The association of preterm labor with vaginal colonization of group B streptococci. Iranian Journal of Reproductive Medicine. 2007;5(4):191.
12. Jahed T, Shariati MK, Darabi P, Karimi A. Frequency of Group B Streptococcus colonization and antibiogram in women at 35-37 weeks of gestation visited in prenatal clinic of Mahdieh Hospital in 2008. Pajoohandeh Journal. 2011;16(3):139-43. URL: http://pajoohande.sbmu.ac.ir/article-1-1164-en.html.
13. Absalan M, Eslami G, Zandi H, Mosaddegh A, Vakili M, Khalili MB. Prevalence of Recto-Vaginal Colonization of Group B Streptococcus in Pregnant Women. Journal of Isfahan Medical School. 2013;30(220).
14. Lu B, Li D, Cui Y, Sui W, Huang L, Lu X. Epidemiology of G roup B streptococcus isolated from pregnant women in B eijing, C hina. Clinical Microbiology and Infection. 2014;20(6):O370-O3. DOI: 10.1111/1469-0691.12416.
15. Busetti M, D’Agaro P, Campello C. Group B streptococcus prevalence in pregnant women from North-Eastern Italy: advantages of a screening strategy based on direct plating plus broth enrichment. Journal of clinical pathology. 2007;60(10):1140-3. doi: 10.1136/jcp.2006.043489.
16. Rahnama S, Tehrani H, Amirmozafari N, Azadmanesh K, Biglari S. Distribution of capsular serotypes in group b streptococci clinical isolates based on genotyping. Razi Journal of Medical Sciences. 2010;17(78/79):25-33. URL: http://rjms.iums.ac.ir/article-1-1604-fa.html.
17. Bland ML, Vermillion ST, Soper DE. Late third-trimester treatment of rectovaginal group B streptococci with benzathine penicillin G. American journal of obstetrics and gynecology. 2000;183(2):372-6. DOI: 10.1067/mob.2000.107668.
18. Dahan-Saal J, Gérardin P, Robillard P, Barau G, Bouveret A, Picot S, et al. Determinants of group B streptococcus maternal colonization and factors related to its vertical perinatal transmission: case-control study. Gynecologie, obstetrique & fertilite. 2011;39(5):281-8. DOI: 10.1016/j.gyobfe.2011.02.014.
19. Sharmila V, Joseph NM, Babu TA, Chaturvedula L, Sistla S. Genital tract group B streptococcal colonization in pregnant women: a South Indian perspective. The Journal of Infection in Developing Countries. 2011;5(08):592-5.
20. Beigverdi R, Jabalameli F, Mirsalehian A, Hantoushzadeh S, Boroumandi S, Taherikalani M, et al. Virulence factors, antimicrobial susceptibility and molecular characterization of Streptococcus agalactiae isolated from pregnant women. Acta microbiologica et immunologica Hungarica. 2014;61(4):425-34. https://doi.org/10.1556/AMicr.61.2014.4.4.
21. Nahaei MR, Ghandchilar N, Bilan N, Ghahramani P. Maternal carriage and neonatal colonization of Streptococcus agalactiae in Tabriz, Northwest Iran. Iranian Journal of Medical Sciences. 2015;32(3):177-81.
22. Mousavi SM, Nassaj M, Arabestani MR, Nazeri H, Rahmaninan M, Hoseinzadeh H. Genotyping of Clinical Streptococcus agalactiae Strains Based on Molecular Serotype of Capsular (cps) Gene Cluster Sequences Using PCR Assay in Hamadan during 2013-2014. Infection, Epidemiology and Microbiology. 2016;2(4):12-6. URL: http://journals.modares.ac.ir/article-4-7183-en.html.
23. Kong F, Ma L, Gilbert GL. Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization. Journal of medical microbiology. 2005;54(12):1133-8. DOI: 10.1099/jmm.0.46244-0.
24. Eren A, Kucukercan M, Oguzoglu N, Unal N, Karateke A. The carriage of group B streptococci in Turkish pregnant women and its transmission rate in newborns and serotype distribution. Turk J Pediatr. 2005;47(1):28-33.
25. Ippolito DL, James WA, Tinnemore D, Huang RR, Dehart MJ, Williams J, et al. Group B streptococcus serotype prevalence in reproductive-age women at a tertiary care military medical center relative to global serotype distribution. BMC infectious diseases. 2010;10(1):336. doi: 10.1186/1471-2334-10-336.
| Files | ||
| Issue | Vol 57, No 9 (2019) | |
| Section | Articles | |
| DOI | https://doi.org/10.18502/acta.v57i9.2641 | |
| Keywords | ||
| Group B streptococcus Multiplex polymerase chain reaction (PCR) Non-pregnant women Capsular serotyping | ||
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How to Cite
1.
Goudarzi L, Khalili M bagher, Vakili M, Sadeh M. Prevalence and Serotype Determination of Streptococcus agalactiae Isolated From Non-Pregnant Women in Tehran, Iran. Acta Med Iran. 2020;57(9):560-564.
