Original Articles

An Evaluation of the Levels of IL-1β and IL-7 in Children With Viral Gastroenteritis

Abstract

Gastroenteritis is associated with high rates of morbidity and mortality in the general population, especially in children. To investigate the predictive role of interleukins (IL-1β and IL-7) in children with viral gastroenteritis as potential diagnostic biomarkers. This cross-sectional study included children who were diagnosed with gastroenteritis at private clinics and Al-Batoul Teaching Hospital for Maternity and Childhood, Diyala, Iraq. The present study was conducted from May 2023 to May 2024. Ninety blood samples were collected from children with gastroenteritis, and 50 blood samples were taken from healthy children and those in the control group. Additionally, 90 samples of the stool were collected. The ELIZA technique was used for exploring IL-1β and IL-7 in blood samples and for detecting viral antigens (Norovirus, Adenovirus and Astrovirus) in stool samples. The data were analyzed statistically via SPSS v. 24 and Prism v. 10. There was a statistically significant increase in the incidence of infectious gastroenteritis in thin children aged 1-3 years who were living in rural areas. As indicated in this study, the most common symptoms were diarrhoea, vomiting and fever, and the differences were significant. Compared with those in the control group, there were significant differences in the levels of IL-1β and IL-7 among the children infected with gastroenteritis. ROC analysis of IL-1β and IL-7 interleukins revealed moderate sensitivity (79% and 88%, respectively) and low specificity (64% and 69%, respectively) at cut-off values of 8.52 and 7.01. The occurrence of norovirus occurred in 30% of the total samples, followed by Adenovirus in 20% and Astrovirus in 11%. The serum levels of IL-1β and IL-7 were high among children infected with gastroenteritis. Nevertheless, these parameters cannot be considered reliable vital indicators that are sufficient for diagnosing infections caused by gastroenteritis.

1. Soltan Dallal MM, Rajabi Z, Papizadeh M, Amiri S, Foroushani AR, Naser A, et al. Prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli associated with outbreaks of food-borne gastroenteritis in Tehran. Cell Mol Biomed Rep 2024;4:199-207.
2. Horne RG, Freedman SB, Johnson-Henry KC, Pang XL, Lee BE, Farion KJ, et al. Intestinal microbial composition of children in a randomized controlled trial of probiotics to treat acute gastroenteritis. Front Cell Infect Microbiol 2022;12:883163.
3. Monroe SS. Control and prevention of viral gastroenteritis. Emerg Infect Dis 2011;17:1347-8.
4. Leung A, Prince T, Society CP, Nutrition, Committee G. Oral rehydration therapy and early refeeding in the management of childhood gastroenteritis. Paediatr Child Health 2006;11:527-31.
5. Koteswari P, Lakshmi PA, Yaseen M, Sultana S, Tabassum A, Soumya P, et al. Preterm birth: causes and complications observed in tertiary care hospitals. Cell Mol Biomed Rep 2022;2:202-12.
6. Amjadi N, Talayeh M, Momeni M, Mansouri N. The comparison of umbilical cord artery pH in newborns with and without thick meconium stained amniotic fluid. Cell Mol Biomed Rep 2023;3:222-6.
7. Bányai K, Estes MK, Martella V, Parashar UD. Viral gastroenteritis. Lancet 2018;392:175-86.
8. Huang Y, Zhou N, Zhang S, Yi Y, Han Y, Liu M, et al. Norovirus detection in wastewater and its correlation with human gastroenteritis: a systematic review and meta-analysis. Environmental Science and Pollution Research. 2022;29:22829-42.
9. Kaminsky LW, Al-Sadi R, Ma TY. IL-1β and the intestinal epithelial tight junction barrier. Front Immunol 2021;12:767456.
10. Isaacs KL, Sartor RB, Haskill S. Cytokine messenger RNA profiles in inflammatory bowel disease mucosa detected by polymerase chain reaction amplification. Gastroenterology 1992;103:1587-95.
11. Mao L, Kitani A, Strober W, Fuss IJ. The role of NLRP3 and IL-1β in the pathogenesis of inflammatory bowel disease. Front Immunol 2018:9:2566.
12. Rawat M, Nighot M, Al-Sadi R, Gupta Y, Viszwapriya D, Yochum G, et al. IL1B increases intestinal tight junction permeability by up-regulation of MIR200C-3p, which degrades occludin mRNA. Gastroenterology 2020;159:1375-89.
13. Man SM. Inflammasomes in the gastrointestinal tract: infection, cancer and gut microbiota homeostasis. Nat Rev Gastroenterol Hepatol 2018;15:721-37.
14. Outlioua A, Badre W, Desterke C, Echarki Z, El Hammani N, Rabhi M, et al. Gastric IL-1β, IL-8, and IL-17A expression in Moroccan patients infected with Helicobacter pylori may be a predictive signature of severe pathological stages. Cytokine 2020;126:154893.
15. Zhang W, Du JY, Yu Q, Jin JO. Interleukin-7 produced by intestinal epithelial cells in response to Citrobacter rodentium infection plays a major role in innate immunity against this pathogen. Infect Immun 2015;83:3213-23.
16. Fry TJ, Mackall CL. The many faces of IL-7: from lymphopoiesis to peripheral T cell maintenance. The J Immunol 2005;174:6571-6.
17. Lee JC, Lyons PA, McKinney EF, Sowerby JM, Carr EJ, Bredin F, et al. Gene expression profiling of CD8+ T cells predicts prognosis in patients with Crohn disease and ulcerative colitis. J Clin Invest 2011;121:4170-9.
18. Belarif L, Danger R, Kermarrec L, Nerrière-Daguin V, Pengam S, Durand T, et al. IL-7 receptor influences anti-TNF responsiveness and T cell gut homing in inflammatory bowel disease. J Clin Invest 2019;129:1910-25.
19. Lin FJ, Huang YC, Huang YC, Huang LM, Liu CC, Chi H, et al. Clinical and epidemiological features in hospitalized young children with acute gastroenteritis in Taiwan: a multicentered surveillance through 2014–2017. J Formos Med Assoc 2022;121:519-28.
20. Khan MA. Epidemiological studies on gastroenteritis in children in the Bannu district, Khyber Pakhtunkhwa, Pakistan. Z Gesundh Wiss 2023;31:739-46.
21. Cai H, Shao Y, Yu W. Prevalence and associated factors of acute gastroenteritis in children and adolescents aged from 6 to 17 years old: a cross-sectional study based on the National Health and Nutrition Examination Survey database 1999–2018. BMJ Open 2023;13:e068319.
22. Chaucheyras-Durand F, Sacy A, Karges K, Apper E. Gastro-intestinal microbiota in equines and its role in health and disease: The black box opens. Microorganisms 2022;10:2517.
23. Moon RC, Bleak TC, Rosenthal NA, Couturier B, Hemmert R, Timbrook TT, et al. Relationship between diagnostic method and pathogen detection, healthcare resource use, and cost in US adult outpatients treated for acute infectious gastroenteritis. J Clin Microbiol 2023;61:e01628-22.
24. Robson K, Bouchoucha S, Considine J. Emergency department assessment and management of children with gastroenteritis. Australas Emerg Care 2024;27:81-7.
25. Leung AK, Hon KL. Paediatrics: how to manage viral gastroenteritis. Drugs Context 2021;10:2020-11-7.
26. Kılıçaslan O, Sav N, Karaca S, Şahin I, Öksüz S, Kocabay K. Rol e of routine laboratory markers in the diagnosis of rotavirus and adenoviru s gastroenteritis. J Med Sci Res 2022;10:76-81.
27. Koo HL, Neill FH, Estes MK, Munoz FM, Cameron A, DuPont HL, et al. Noroviruses: the most common pediatric viral enteric pathogen at a large university hospital after introduction of rotavirus vaccination. J Pediatric Infect Dis Soc 2013;2:57-60.
28. Al-Saffar VH. Rotavirus Stimulates Secretion of Interleukin-1beta (IL-1β) in Children with Gastroenteritis. Int J Psychosoc Rehabil 2020;24.
29. Lu Z, Zou J, Hu Y, Li S, Zhou T, Gong J, et al. Serological markers associated with response to immune checkpoint blockade in metastatic gastrointestinal tract cancer. JAMA Netw Open 2019;2:e197621.
30. Hyams JS, Fitzgerald JE, Wyzga N, Treem WR, Justinich CJ, Kreutzer DL. Characterization of circulating interleukin-1 receptor antagonist expression in children with inflammatory bowel disease. Dig Dis Sci 1994;39:1893-9.
31. Raab Y, Hällgren R, Gerdin B. Enhanced Intestinal Synthesis of lnterleukin-6 Is Related to the Disease Severity and Activity in Ulcerative Colitis. Digestion 1994;55:44-9.
32. Wedrychowicz A, Stopyrowa J, Fyderek K, Miezynski W. Stool interleukin 1β and interleukin 1 receptor antagonist concentrations in children with active ulcerative colitis and during recovery. Eur J Pediatr 2003;162:541-2.
33. Villatoro A, Cuminetti V, Bernal A, Torroja C, Cossío I, Benguría A, et al. Endogenous IL-1 receptor antagonist restricts healthy and malignant myeloproliferation. Nat Commun 2023;14:12.
34. Bednarz-Misa I, Diakowska D, Krzystek-Korpacka M. Local and systemic IL-7 concentration in gastrointestinal-tract cancers. Medicina (Kaunas) 2019;55:262.
35. Winer H, Rodrigues GO, Hixon JA, Aiello FB, Hsu TC, Wachter BT, et al. IL-7: comprehensive review. Cytokine 2022;160:156049.
36. Shalapour S, Deiser K, Kühl AA, Glauben R, Krug SM, Fischer A, et al. Interleukin-7 links T lymphocyte and intestinal epithelial cell homeostasis. PloS One 2012;7:e31939.
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IssueVol 63 No 1 (2025) QRcode
SectionOriginal Articles
DOI https://doi.org/10.18502/acta.v63i1.18590
Keywords
Gastroenteritis Viral infections Interleukins Interleukin-1 beta (IL-1 β) Interleukin-7 (IL-7)
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How to Cite
1.
Mohsen SM, Hwaid AH, Nazzal MF. An Evaluation of the Levels of IL-1β and IL-7 in Children With Viral Gastroenteritis. Acta Med Iran. 2025;63(1):19-24.