Correlation Between ncRNA Gene Profiles and NEIL1 Expression in Breast Cancer
Abstract
Endonuclease VIII-like 1, a crucial DNA repair enzyme encoded by the Neil1 gene, is essential for shielding the genome from oxidative damage. It is an essential part of the Base Excision Repair (BER) pathway, which fixes minor but dangerous DNA defects. We describe how the Neil family contributes to cancer’s harmful biological characteristics. The aim is to detect some types of ncRNA genes that can be used as molecular markers for breast cancer. The methodology involves detecting the gene expression levels of the Neil1 gene and several ncRNA genes, including miRNA‑3912‑3p, snoRNA SNHG7, and lncRNA BC069792, using the qPCR technique. The results showed increased expression levels of the Neil1 gene (2.435), miRNA‑3912‑3p (12.34), lncRNA BC069792 (6.11), and snoRNA SNHG7 (5.08) in patients with biopsy samples, while decreased expression levels were observed for all genes in blood samples compared with healthy cases. The conclusion from this study suggests that miRNA‑3912‑3p may be considered a regulatory factor for the Neil1 gene and a potential biomarker for breast cancer.
2. Shah AW, Fatima E. Cellular mechanisms of DNA repair in response to genotoxic stress. J Transl Res 2024;2:92-114.
3. Hamed OM. Analysis of common mutation of P53 gene in males with lung cancer in Mosul City. Bionatura 2022;7:52.
4. Caliri AW, Tommasi S, Besaratinia A. Relationships among smoking, oxidative stress, inflammation, macromolecular damage, and cancer. Mutat Res Rev Mutat Res 2021;787:108365.
5. Yuan H, Qing T, Zhu S, Yang X, Wu W, Xu K, et al. The effects of altered DNA damage repair genes on mutational processes and immune cell infiltration in esophageal squamous cell carcinoma. Cancer Med 2023;12:10077-90.
6. Hameed MA, Hamed OM. Detection of P53 suppressor gene mutation in women with breast cancer in Mosul city. AIP Conf Proc 2023;2834:020007.
7. Hua AB, Sweasy JB. Functional roles and cancer variants of the bifunctional glycosylase NEIL2. Environ Mol Mutagen 2023;65:40-56.
8. Grundy GJ, Parsons JL. Base excision repair and its implications to cancer therapy. Essays Biochem 2020;64:831-43.
9. Ramadan ZJ, Hamed OM, Khalaf IH. Detection of genetic variation for some genes related with recurrent spontaneous abortion in Nineveh province. Biochem Cell Arch 2020;20:6407-14.
10. Chen Y, Ma M, Zou A, Wang X, Dong W. Research progress on the role of the NEIL family in cancer. Front Cell Dev Biol 2025;13:1612329.
11. Eckenroth BE, Bumgarner JD, Matsumoto-Elliott O, David SS, Doublié S. Structural and biochemical insights into NEIL2’s preference for abasic sites. Nucleic Acids Res 2023;51:12508-21.
12. Khudher S, Hamed OM. The effect of non-coding RNA genes on the gene expression of MLH1 gene in patients with lung cancer. Health Biotechnol Biopharma 2025;9:1-13.
13. Cherbuin N, Patel H, Walsh EI, Ambikairajah A, Burns R, Brüstle A, Rasmussen LJ. Cognitive function is associated with genetically determined efficiency of DNA repair mechanisms. Genes 2024;15:153.
14. Duijf PH, Nanayakkara D, Nones K, Srihari S, Kalimutho M, Khanna KK. Mechanisms of genomic instability in breast cancer. Trends Mol Med 2019;25:595-611.
15. Kang L, Zou X, Zhang G, Xiang J, Wang Y, Yang M, et al. A variant in a microRNA binding site in NEIL2 3′UTR confers susceptibility to age‑related cataracts. FASEB J 2019;33:10469-76.
16. Anurag M, Punturi N, Hoog J, Bainbridge MN, Ellis MJ, Haricharan S. Comprehensive profiling of DNA repair defects in breast cancer identifies a novel class of endocrine therapy resistance drivers. Clin Cancer Res 2018;24:4887-99.
17. Yamamoto R, Yamamoto M, Kusaka H, Masatsugu H, Matsuyama S, Tajima T, et al. NEIL1 mRNA splicing variants are expressed in normal mouse organs. J Radiat Res 2012;53:234-41.
18. Mohajeri Khorasani A, Raghibi A, Haj Mohammad Hassani B, Bolbolizadeh P, Amali A, Sadeghi M, et al. Decoding the role of NEIL1 gene in DNA repair and lifespan: a literature review with bioinformatics analysis. Adv Biol 2024;8:2300708.
19. Al-Hassani OMH. Role of MTHFR C667T and MTRR A66G genes polymorphism with thyroid disorders. J Phys Conf Ser 2020;1660:012007.
20. Prakash A, Carroll BL, Sweasy JB, Wallace SS, Doublié S. Genome and cancer single nucleotide polymorphisms of the human NEIL1 DNA glycosylase: activity, structure, and the effect of editing. DNA Repair (Amst) 2014;14:17-26.
| Files | ||
| Issue | Vol 64 No 4 (2026) | |
| Section | Original Articles | |
| Keywords | ||
| Breast cancer Base excision repair miRNA3912-3p snoRNA SNHG7 lncRNA BC069792 Neil1 gene | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
