Evaluation of Angiogenesis in Colorectal Carcinoma by CD34 Immunohistochemistry Method and its Correlation with Clinicopathologic Parameters

  • Nourieh Sharifi Department of Pathology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Kamran Ghaffarzadegan Department of Pathology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Hossein Ayatollahi Mail Department of Pathology and Hematology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Mohammad Taghi Shakeri Department of Community Medicine, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Mohammad Hadi Sadeghian Department of Pathology and Hematology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Jafar Bordbar Azari Department of Pathology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Keywords:
Angiogenesis, antigens, CD34, colorectal neoplasm, immunohistochemistry, clinical pathology

Abstract

The basic pathogenic step in the process of tumor growth, invasion and metastasis is tumor-induced angiogenesis. The aim of this study was to evaluate the angiogenesis in colorectal carcinoma by microvascular density (MVD) determination with IHC (immunohistochemistry) method and to determine if and how angiogenesis correlates with clinicopathologic parameters. Sixty two archival, paraffin embedded tissue samples of colorectal carcinoma from Omid Hospital (Mashhad, Islamic republic of Iran) were selected. Microvessels were identified immunohistochemically, using monoclonal CD34 antibody. Two investigators examined the microvessel density then the median value of MVD was determined and correlated with clinicopathologic parameters. Tumor-induced angiogenesis of colorectal carcinoma statistically correlated with histological tumor grade (P=0.000). There was no significant correlation between intratumoral microvessel density and sex and age of patients, localization, and stage and histologicalal tumor type (P > 0.1). Intratumoral microvessel density quantification in histologic specimens of colorectal carcinoma reflects the grade of tumors and may be a useful additional prognostic factor.

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How to Cite
1.
Sharifi N, Ghaffarzadegan K, Ayatollahi H, Shakeri MT, Sadeghian MH, Bordbar Azari J. Evaluation of Angiogenesis in Colorectal Carcinoma by CD34 Immunohistochemistry Method and its Correlation with Clinicopathologic Parameters. Acta Med Iran. 47(3):161-164.
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