Temperature Changes During and after Eccentric Contractions and its Effect on Force and Desmin Loss in Rat
The typical features of eccentric exercise-induced muscle damage is prolonged loss of muscle strength and the most rapid structural change in the fibers is loss of immunostaining for the intermediate filament protein, desmin. In this study isolated perfused rat muscle was used to examine the direct effect of temperature changes on the eccentric contraction-induced force and desmin loss. The left medial gastrocnemius muscle was separated and the entire lower limb was transferred into a prewarmed (35o C) organ bath. Temperature was adjusted to 31 or 39o C during and after eccentric contractions. Maximal isometric force and desmin loss were measured after 15 isometric or eccentric contractions. According to our data, organ bath temperature changes during or after eccentric contractions had no significant effect on force loss. However, a strong correlation between desmin loss and temperature changes during (r = 0.886, P< 0.05) and a weak correlation between desmin loss and temperature changes after (r= 0.699, P<0.05) eccentric contractions was observed. Our results suggest that cooling during eccentric contractions may decrease desmin loss but temperature changes after eccentric contractions have no effect on desmin loss.
Prosk U, Allen TJ. Damage to skeletal muscle from eccentric exercise. Exer Sport Sci Rev 2001; 33:98-104.
Allen DG. Eccentric muscle damage: mechanisms of early reduction of force. Acta Physiol Scand 2001; 171:311-9.
Lieber RL, Fridén J. Mechanisms of muscle injury gleaned from animal models. Am J Phys Med Rehabil 2002; 81:S70-S79.
Evans RK, Knight KL, Draper DO, Parcell AC. Effects of warm-up before eccentric exercise on indirect markers of muscle damage. Med Sci Sports Exerc 2002; 34:1892-9.
Armstrong RB, Warren GL, Warren JA. Mechanisms of exercise-induced muscle fiber injury. Sports Med. 1991; 12:184-207.
Fridén J, Lieber RL. Segmental muscle fiber lesions after repetitive eccentric contractions. Cell Tissue Res 1998; 293:165-71.
Friden J, Lieber RL. Eccentric exercise-induced injuries to contractile and cytoskeletal muscle fiber components. Acta Physiol Scand 2001; 171:321-6.
Price MG. Molecular analysis of intermediate filament cytoskeleton-putative load-bearing structure. Am J Physiol= 1984; 246:H566-H72.
Belcastro AN. Skeletal muscle calcium-activated neutral protease (calpain) with exercise. J Appl Physiol. 1993; 74:1381-86.
Féason L, Stockholm D, Freyssene D, Richard I, Duguez S, Beckmann JS, Denis C. Molecular adaptations of neuromuscular disease-associated proteins in response to eccentric exercise in human skeletal muscle. J Physiol 2002; 5431:297-306.
Ray CA, Gracey KH. Augmentation of exercise-induced muscle sympathetic nerve activity during muscle heating. J Appl Physiol 1997; 82:1719-25.
Ray CA, Hume KM, Gracey K H, Mahoney ET.. Muscle cooling delays activation of the muscle metaboreflex in humans. Am J Physiol 1997; 273:H2436-41.
Warren GL, Ingalls CP, Armstrong RB. Temperature dependency of force loss and Ca (2+) homeostasis in mouse EDL muscle after eccentric contractions. Am. J. Physiol. Regul. Integr. Comp. Physiol 2002; 282:R1122-R32.
Blazevich AJ, Sharp NCC. Understanding Muscle Architectural Adaptation: Macro-and Micro-Level Research. Cells Tissues Organs 2005; 181:1-10.
Nosaka K, Sakamoto K, Mewton M, Sacco P. Influence of Pre-Exercise Muscle Temperature on Responses to Eccentric Exercise. J Athle Training 2004; 39:132-7.
Brock Symons T, Clasey JL, Gater DR, Yates JW. Effects of deep heat as a preventative mechanism on delayed onset muscle soreness. J Strength Cond Res 2004; 18:155-61.
Paddon-Jones D, Quigley BM. Effect of cryotherapy on muscle soreness and strength following eccentric exercise. Int J Sports Med 1997; 18:588-93.
Rijkelijkhuizen JM, Baan GC, de Haan A, de Ruiter CJ, Huijing PA. Extramuscular myofascial force transmission for in situ rat medial gastrocnemius and plantaris muscles in progressive stages of dissection. J Exp Biol 2005; 208:129-40.
Constable JK, Barclay CJ, Gibbs CL. Energetics of lengthening in mouse and toad skeletal muscles. J Physiol (Lond) 1997; 505:205-15
Delp MD, Duan C, Ray CA, Armstrong RB. Rat hindlimb muscle blood flow during level and downhill locomotion. J Appl Physiol 1999; 86:564-8.
Ranatunga KW, Sharpe B, Turnbull B. Contractions of a human skeletal muscle at different temperatures. J Physiol (Lond) 1987; 390:383-95.
Kenny GP, Reardon FD, Zaleski W, Reardon ML, Haman F, Ducharme MB. Muscle temperature transients before, during, and after exercise measured using an intramuscular multisensor probe. J Appl Physiol 2003; 94:2350-7.
Draper D, Harris St, Schulthies S, Durrant E, Knight KL, Ricard M. Hot-Pack and 1-MHz Ultrasound Treatments Have an Additive Effect on Muscle Temperature Increase. J Athl Train 1998; 33:21-24.
Ashton DF, Draper DO, Myrer JW. Temperature Rise in Human Muscle During Ultrasound Treatments Using Flex- All as a Coupling Agent. J Athl Train 1998; 33:136-40.
Wirth VJ, Van Luen BL, Mistry D, Saliba E, McCue FC. Temperature Changes in Deep Muscles of Humans During Upper and Lower Extremity Exercise. J Athl Train 1998; 33:211-5.
Draper DO, Knight K, Fujiwara T, Castel JC. Temperature change in humam muscle during and after pulsed shortwave diathermy. J Orthop Sports Phy Ther 1999; 29: 13- 18;Discussion 19-22.
Warren GL, Lowe DA, Armstrong RB. Measurement tools used in the study of eccentric contraction-induced injury. Sports Med 1999; 27:43-59.
Koh TJ, Escobedo J. Cytoskeletal disruption and small heat shock protein translocation immediately after lengthening contractions. Am J Physiol Cell Physiol 2004; 286:C713-C22.
Yaoita H, Fischman AJ, Strauss HW, Saito T, Sato E, Maruyama Y. Uridine: a marker of myocardial viability after coronary occlusion and reperfusion. Int J Cardiac Imaging. 1993; 9:273-80.
Goldlust EJ, Paczynski RP, He YY, Hsu CY, Goldberg MP. Automated measurement of infarct size with scanned images of triphenyltetrazolium chloride–stained rat brains. Stroke1996; 27:1657-62.
Howell JN, Chleboun G, Conatser R. Muscle stiffness, strength loss, swelling and soreness following exerciseinduced injury in humans. J Physiol (Lond) 1993; 464:183-96.
Proske U, Allen TJ. Damage to skeletal muscle from eccentric exercise. Exerc Sport Sci Rev. 2005; 33:98-104.
Tidus PM, Cort J, Woodruff SJ, Bryden P. Ultrasound Treatment and Recovery From Eccentric-Exercise-Induced Muscle Damage. J Sport Rehabil 2002; 11:305-14.
Vasaghi-Gharamaleki B, Keshavarz M, Gharibzadeh S, Sotodeh M, Marvi H, Mosayebnejad J, Takamjani IE. The influence of temperature alternations on eccentric contraction-induced isometric force and desmin loss in rat medial gastrocnemius muscle. J Med Sci 2008, 8(2): 162-9.
Peters D, Barash IA, Burdi M, Yuan PS, Mathew L, Fridén J, Lieber R. Asynchronous functional, cellular and transcriptional changes after a bout of eccentric exercise in rat. J Physiol 2003; 553:947-57.
Yu JG, Fürst DO, Thornell LE. The mode of myofibril remodelling in human skeletal muscle affected by DOMS induced by eccentric contractions. Histochem Cell Biol 2003; 119:383-93.
Beaton LJ, Tarnopolsky MA, Phillips SM. Contractioninduced muscle damage in humans following calcium channel blocker administration. J Physiol 2002; 544:849-59.
Crameri RM, Aagaard P, Qvortrup K, Langberg H, Olesen J, Kjaer M. Myofiber damage in human skeletal muscle: effects of electrical stimulation versus voluntary contraction. J Physiol 2007; 583:365-80.
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