Acute and Subchronic Toxicity Assessment of the Hydroalcoholic Extract of Stachys lavandulifolia in Mice
Stachys lavandulifolia is used as the herbal tea and its wide and potent medical effects have been reported for the extract in animal studies. This study aimed to find the safety profile of the extract to find the appropriate doses for further human studies. The aerial parts of the plant were air-dried and the hydroalcoholic extract was obtained and concentrated by percolation method with 140 mg/ml concentration. To assess the toxicity profile of this extract, 60 female mice (30 cases, 30 controls, 24.8 ±2.1 g, 4-6 weeks) were administered the extract by oral gavages in acute (24 hrs), subacute (14 days) and subchronic (45 days) models. All clinical, hematological, biochemical and histopathological changes were assessed in appropriate midpoints and endpoints and compared with control group. Doses up to 140 mg⁄kg were recognized as maximum tolerated dose in subchronic model. Abnormal changes in kidney and liver weight in treatment groups as well as the significant elevation of biochemical parameters in 45 days study has suggested the possible hepatic and renal toxicity potentials of this extract with doses upper than 140mg⁄kg. Doses up 70 mg⁄kg could be considered as no observable adverse effect level (NOAEL) and could be used in further clinical trials on the possible therapeutic effects of this plant.
Skaltsa HD, Mavrommati A, Constantinidis T. A chemotaxonomic investigation of volatile constituents in Stachys subsect. Swainsonianeae (Labiatae). Phytochemistry 2001;57(2):235-44.
Skaltsa HD, Lazari DM, Chinou IB, Loukis AE. Composition and antibacterial activity of the essential oils of Stachys candida and S. chrysantha from southern Greece. Planta Med 1999;65(3):255-6.
Mozaffarian V. A dictionary of Iranian plant names. Iran:Farhang Moaser Press; 1996.
Rechinger KH. Labiatae. In: Rechinger KH, editor. Flora Iranica, No. 150. Graz: Akademische, Druck- und Verlagsanstalt; 1982; p. 354-95.
Basaran AA, Calis I, Anklin C, Nishibe S, Sticher O. Lavandulifolioside: a new phenylpropanoid glycoside from Stachys lavandulifolia. Helvetica Chimica Acta1988;71(6):1483-90
Amin GH. Popular Medicinal Plants of Iran. Tehran: Health Ministry Press; 1991.
Rabbani M, Sajjadi SE, Zarei HR. Anxiolytic effects ofStachys lavandulifolia Vahl on the elevated plus-maze model of anxiety in mice. J Ethnopharmacol 2003;89(2-3):271-6.
Rabbani M, Sajjadi SE, Jalali A. Hydroalcohol extract and fractions of Stachys lavandulifolia Vahl: effects on spontaneous motor activity and elevated plus-maze behaviour. Phytother Res 2005;19(10):854-8.
Saeedi M, Morteza-Semnani K, Mahdavi MR, Rahimi F. Antimicrobial studies on extracts of four species of stachys. Indian J Pharm Sci 2008;70(3):403-6.
Khanavi M, Sharifzadeh M, Hadjiakhoondi A, Shafiee A. Phytochemical investigation and anti-inflammatory activity of aerial parts of Stachys byzanthina C. Koch. J Ethnopharmacol 2005;97(3):463-8.
Ramezani M, Hassanzadeh MK and Safdarabadi DM. Volatile constituents of Stachys lavandulifolia Vahlgrowing in Iran. ACGC Chem Res Commun 2002;15:15:20-3.
Javidnia K, Mojab F, Mojahedi SA. Chemical constituents of the essential oil of Stachys lavandulifolia Vahl from Iran. Iran J Pharmaceutical Res 2004;3:61-3.
Delazar A, Delnavazi MR, Nahar L, Moghadam SB, Mojarab M, Gupta A, Williams AS, Mukhlesur Rahman M, Sarker SD. Lavandulifolioside B: a new phenylethanoid glycoside from the aerial parts of Stachys lavandulifolia Vahl. Nat Prod Res 2011;25(1):8-16.
McNaught AD, Wilkinson A. Compendium of ChemicalTerminology: IUPAC. Recommendations. 2nd ed. Oxford: Blackwell Scientific Publications; 1997.
Altun D, Ayar A, Uysal H, Kara AA, Unal EL. Extended longevity of Drosophila melanogaster by water and ethanol extracts of Stachys lavandulifolia. Pharm Biol 2010;48(11):1291-6.
Hajhashemi V, Ghannadi A, Sedighifar S. Analgesic and anti-inflammatory properties of the hydroalcoholic, polyphenolic and boiled extracts of Stachys lavandulifolia.Res Pharmaceutical Sci (RPS) 2007;2:92-8.
Ghasemi Pirbalouti A, Roshan Chaleshtori A, Tajbakhsh E, Momtaz H, Rahimi E, Shahin F. Bioactivity of medicinal plants extracts against Listeria monocytogenes isolated from food. Int J Food Agriculture Environment (JFAE) 2009;7(1):66-9.
Naghibi F, Mosaddegh M, Motamed SM, Ghorbani A. Labiatae family in folk medicine in Iran: from ethnobotany to pharmacology. Iran J Pharmaceutical Res 2005;4(2): 63-79.
Maleki N, Garjani A, Nazemiyeh H, Nilfouroushan N, Eftekhar Sadat AT, Allameh Z, Hasannia N. Potent antiinflammatory activities of hydroalcoholic extract from aerial parts of Stachys inflata on rats. J Ethnopharmacol 2001;75(2-3):213-8.
|Issue||Vol 49, No 12 (2011)|
|Stachys lavandulifolia Acute toxicity Subacute toxicity Subchronic toxicity NOAEL|
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