Effect of Topical Application of Silymarin (Silybum marianum) on Excision Wound Healing in Albino Rats
-
Roya Sharifi
,
Hossein Rastegar
,
Mohammad Kamalinejad
,
Ahmad Reza Dehpour
,
Seyed Mohammad Tavangar
,
Maliheh Paknejad
,
Mahboobeh Mehrabani Natanzi
,
Naghmeh Ghannadian
,
Minoo Akbari
,
Parvin Pasalar
Abstract
Silymarin, an extract from Silybum marianum, has been shown to have antioxidant properties. However, there is no scientific report on wound healing activity of the silymarin. The purpose of this study was to evaluate the effect of topical administration of silymarin on excision wound healing in rats. Excision wounds were made on the back of rats. Rats were divided into three groups, as control, vehicle, and treatment. Vehicle and treatment groups received polyethylene glycol and silymarin dissolved in polyethylene glycol, respectively. The control group did not receive any treatment. The wound tissues were removed on 5th, 10th and 15th day for histopathological analysis and total collagen determination by hydroxyproline assay. Results showed that silymarin increased epithelialization and decreased inflammation but did not have any effect on percentage of wound contraction, collagenization and hydroxyproline levels. It was concluded that silymarin can significantly stimulate epithelialization and reduce inflammation in full-thickness wounds in rats.
Diegelmann RF, Evans MC. Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci 2004;9:283-9.
James TJ, Hughes MA, Hofman D, Cherry GW, Taylor RP. Antioxidant characteristics of chronic wound fluid. Br J Dermatol 2001;145(1):185-6.
Musalmah M, Fairuz AH, Gapor MT, Ngah WZ. Effect of vitamin E on plasma malondialdehyde, antioxidant enzyme levels and the rates of wound closures during wound healing in normal and diabetic rats. Asia Pac J Clin Nutr 2002;11 Suppl 7:S448-51.
Lodhi S, Pawar RS, Jain AP, Singhai A. Wound healing potential of Tephrosia purpurea (Linn.) Pers. in rats. J Ethnopharmacol 2006;108(2):204-10.
Majumdar M, Nayeem N, Kamath JV, Asad M. Evaluation of Tectona grandis leaves for wound healing activity. Pak J Pharm Sci 2007;20(2):120-4.
Suguna L, Singh S, Sivakumar P, Sampath P, Chandrakasan G. Influence of Terminalia chebula on dermal wound healing in rats. Phytother Res 2002;16(3):227-31.
Gazák R, Walterová D, Kren V. Silybin and silymarin: New and emerging applications in medicine. Curr Med Chem 2007;14(3):315-38.
Gu M, Singh RP, Dhanalakshmi S, Agarwal C, Agarwal R. Silibinin inhibits inflammatory and angiogenic attributes in photocarcinogenesis in SKH-1 hairless mice. Cancer Res 2007;67(7):3483-91.
Katiyar SK. Treatment of silymarin, a plant flavonoid, prevents ultraviolet light-induced immune suppression and oxidative stress in mouse skin. Int J Oncol 2002;21(6):1213-22.
Katiyar SK, Korman NJ, Mukhtar H, Agarwal R. Protective effects of silymarin against photocarcinogenesis in a mouse skin model. J Natl Cancer Inst 1997;89(8):556-66.
Meeran SM, Katiyar S, Elmets CA, Katiyar SK. Silymarin inhibits UV radiation-induced immunosuppression through augmentation of interleukin-12 in mice. Mol Cancer Ther 2006;5(7):1660-8.
Singh RP, Agarwal R. Flavonoid antioxidant silymarin and skin cancer. Antioxid Redox Signal 2002;4(4):655-63.
Svobodová A, Walterová D, Psotová J. Influence ofsilymarin and its flavonolignans on H(2)O(2)-induced oxidative stress in human keratinocytes and mousefibroblasts. Burns 2006;32(8):973-9.
Svobodová A, Zdarilová A, Malisková J, Mikulková H, Walterová D, Vostalová J. Attenuation of UVA-induced damage to human keratinocytes by silymarin. J Dermatol Sci 2007;46(1):21-30.
Toklu HZ, Tunali-Akbay T, Erkanli G, Yüksel M, Ercan F, Sener G. Silymarin, the antioxidant component of Silybum marianum, protects against burn-induced oxidative skin injury. Burns 2007;33(7):908-16.
Zhao J, Lahiri-Chatterjee M, Sharma Y, Agarwal R. Inhibitory effect of a flavonoid antioxidant silymarin on benzoyl peroxide-induced tumor promotion, oxidative stress and inflammatory responses in SENCAR mouse skin. Carcinogenesis 2000;21(4):811-6.
Abramov Y, Golden B, Sullivan M, Botros SM, Miller JJ, Alshahrour A, Goldberg RP, Sand PK. Histologic characterization of vaginal vs. abdominal surgical wound healing in a rabbit model. Wound Repair Regen 2007;15(1):80-6.
Woessner JF Jr. The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch Biochem Biophys 1961;93:440-7.
Cardoso CR, Souza MA, Ferro EA, Favoreto S Jr, Pena JD. Influence of topical administration of n-3 and n-6 essential and n-9 nonessential fatty acids on the healing of cutaneous wounds. Wound Repair Regen 2004;12(2):235-43.
Swaim SF, Lee AH. Topical wound medications: a review. J Am Vet Med Assoc 1987;190(12):1588-93.
Swaim SF, Hinkle SH, Bradley DM. Wound contraction: basic and clinical factors. Compend Contin Educ Pract Vet 2001;23(1):20-4.
Russo A, Longo R, Vanella A. Antioxidant activity of propolis: role of caffeic acid phenethyl ester and galangin. Fitoterapia 2002;73 Suppl 1:S21-9.
Ramakrishnan G, Raghavendran HR, Vinodhkumar R, Devaki T. Suppression of N-nitrosodiethylamine induced hepatocarcinogenesis by silymarin in rats. Chem Biol Interact 2006;161(2):104-14.
Nencini C, Giorgi G, Micheli L. Protective effect of silymarin on oxidative stress in rat brain. Phytomedicine 2007;14(2-3):129-35.
Cotran RS, Kumar V, Robbins SL, Schoen FJ, editors. Robbins Pathologic Basis of Disease. Philadelphia, PA: WB Saunders; 1994. p. 35-92.
Eming SA, Werner S, Bugnon P, Wickenhauser C, Siewe L, Utermöhlen O, Davidson JM, Krieg T, Roers A. Accelerated wound closure in mice deficient for interleukin-10. Am J Pathol 2007;170(1):188-202.
Gutiérrez-Fernández A, Inada M, Balbín M, Fueyo A,Pitiot AS, Astudillo A, Hirose K, Hirata M, Shapiro SD, Noël A, Werb Z, Krane SM, López-Otín C, Puente XS. Increased inflammation delays wound healing in mice deficient in collagenase-2 (MMP-8). FASEB J 2007;21(10):2580-91.
Chang JW, Kim CS, Kim SB, Park SK, Park JS, Lee SK. Proinflammatory cytokine-induced NF-kappaB activation in human mesangial cells is mediated through intracellular calcium but not ROS: effects of silymarin. Nephron Exp Nephrol 2006;103(4):e156-65.
Schümann J, Prockl J, Kiemer AK, Vollmar AM, Bang R, Tiegs G. Silibinin protects mice from T cell-dependent liver injury. J Hepatol 2003;39(3):333-40.
Hashem MA, Jun KY, Lee E, Lim S, Choo HY, Kwon Y. A rapid and sensitive screening system for human type I collagen with the aim of discovering potent anti-aging or anti-fibrotic compounds. Mol Cells 2008;26(6):625-30.
| Files | ||
| Issue | Vol 50, No 9 (2012) | |
| Section | Articles | |
| Keywords | ||
| Epithelialization Excision wound Silymarin Wound healing | ||
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