Effect of Aqueous Extract of Elaeagnus angustifolia Fruit on Experimental Cutaneous Wound Healing in Rats


The present study was conducted to investigate the histological changes and wound healing effect of aqueous extract of Elaeagnus angustifolia. After creating full-thickness skin wounds on the back of 45 male Sprague-Dawley rats they were randomly divided into three groups. Treated group received the extract, positive control group were treated with mupirocin ointment 2% and control group did not receive any treatment. Wound healing rates were calculated on days 3, 5, 8, 10, 12 and 15 post-wounding and the wound tissues were harvested at 5, 10, and 15 days for histological analysis and hydroxyproline content measurement. The results indicated a significant increase in the percentage of wound contraction and hydroxyproline content in the treated group comparing to the control and positive control groups. A significant increase in the assigned histological scores was observed at 10 and 15 days in the treated and positive control groups compared to the control group. The results demonstrate that aqueous extract of Elaeagnus angustifolia accelerates cutaneous wound healing, and its effect may be due to the increased re-epithelialization and collagen deposition in wound and so it can be considered as a therapeutic agent for wound healing.

Guo S, Dipietro LA. Factors affecting wound healing. J Dent Res 2010;89(3):219-29.

Menke NB, Ward KR, Witten TM, Bonchev DG, Diegelmann RF. Impaired wound healing. Clin Dermatol 2007;25(1):19-25.

Gál P, Toporcer T, Grendel T, Vidová Z, Smetana K Jr, Dvoránková B, Gál T, Mozes S, Lenhardt L, Longauer F, Sabol M, Sabo J, Backor M. Effect of Atropa belladonna L. on skin wound healing: biomechanical and histological study in rats and in vitro study in keratinocytes, 3T3 fibroblasts, and human umbilical vein endothelial cells. Wound Repair Regen 2009;17(3):378-86.

Farahbakhsh S, Arbabian S, Emami F, Rastegar Moghadam B, Ghoshooni H, Noroozzadeh A, Sahraei H, Golmanesh L, Jalili C, Zrdooz H. Inhibition of Cyclooxygenase type 1 and 2 enzyme by aqueous extract of Elaeagnus angustifolia in mice. Basic Clin Neuroscience 2011;2(2):31-7.

Dembińska-Migas W, Gill S. Flavonoids in leaves of Elaeagnus angustifolia L. Pol J Pharmacol Pharm 1973;25(6):599-606.

Mirhydar H. Encyclopedia of Plants: Indications of Plants in the Prevention and Treatment of Diseases. Vol. 2. Tehran: Islamic Farhang Press; 1998. p. 163-4.

Gurbuz I, Ustun O, Yesilada E, Sezik E, Kutsal O. Antiulcerogenic activity of some plants used as folk remedy in Turkey. J Ethnopharmacol 2003;88(1):93-7.

Hosseinzadeh H, Ramezani M, Namjo N. Muscle relaxant activity of Elaeagnus angustifolia L. fruit seeds in mice. J Ethnopharmacol 2003;84(2-3):275-8.

Ramezani M, Hosseinzadeh H, Daneshmand N. Antinociceptive effect of Elaeagnus angustifolia fruit seeds in mice. Fitoterapia 2001;72(3):255-62.

Ahmadiani A, Hosseiny J, Semnanian S, Javan M, Saeedi F, Kamalinejad M, Saremi S. Antinociceptive and antiinflammatory effects of Elaeagnus angustifolia fruitextract. J Ethnopharmacol 2000;72(1-2):287-92.

Rasekhi HR, Kamali M, Hosseini SM, Fallahi M. Wound healing properties of Elaeagnus angustifolia. J Pharmacol 1999;51(supplement):128.

Kokane DD, More RY, Kale MB, Nehete MN, Mehendale PC, Gadgoli CH. Evaluation of wound healing activity of root of Mimosa pudica. J Ethnopharmacol 2009;124(2):311-5.

Nayak S. Influence of ethanol extract of Vinca rosea on wound healing in diabetic rats. J Biol Sci 2006;6(3):51-5.

Cardoso CR, Souza MA, Ferro EA, Favoreto S Jr, Pena JD. Influence of topical administration of n-3 and n-6 essential and n-9 nonessential fatty acids on the healing ofcutaneous wounds. Wound Repair Regen 2004;12(2):235-43.

Kim YS, Roh TS, Lee WJ, Yoo WM, Tark KC. The effect of botulinum toxin A on skin flap survival in rats. Wound Repair Regen 2009;17(3):411-7.

Lim JS, Yoo G. Effects of adipose-derived stromal cells and of their extract on wound healing in a mouse model. J Korean Med Sci 2010;25(5):746-51.

Greenhalgh DG, Sprugel KH, Murray MJ, Ross R. PDGFand FGF stimulate wound healing in the geneticallydiabetic mouse. Am J Pathol 1990;136(6):1235-46.

Woessner JF Jr. The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch Biochem Biophys 1961;93: 440-7.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54.

Rassouli A, Shams GR, Sharifi D. Determination ofhydroxyproline in equine tendon using a modifiedspectrophotometric method. Pajouhesh Sazandegi 2007;73:114-9.

Mori R, Kondo T, Nishie T, Ohshima T, Asano M. Impairment of skin wound healing in beta-1,4- galactosyltransferase-deficient mice with reduced leukocyte recruitment. Am J Pathol 2004;164(4): 1303-14.

Edwards R, Harding KG. Bacteria and wound healing. Curr Opin Infect Dis 2004;17(2):91-6.

Beigom Taheri J, Anbari F, Maleki Z, Boostani S, Zarghi A, Pouralibaba F. Efficacy of Elaeagnus angustifolia Topical Gel in the Treatment of Symptomatic Oral Lichen Planus. J Dent Res Dent Clin Dent Prospects 2010;4(1):29-32.

Burgess C. Topical vitamins. J Drugs Dermatol 2008;7(7 Suppl):s2-6.

Martin A. The use of antioxidants in healing. Dermatol Surg 1996;22(2):156-60.

Nayak S, Nalabothu P, Sandiford S, Bhogadi V, Adogwa A. Evaluation of wound healing activity of Allamanda cathartica. L. and Laurus nobilis. L. extracts on rats. BMC Complement Altern Med 2006;6:12.

Jorge MP, Madjarof C, Gois Ruiz AL, Fernandes AT, Ferreira Rodrigues RA, de Oliveira Sousa IM, Foglio MA, de Carvalho JE. Evaluation of wound healing properties of Arrabidaea chica Verlot extract. J Ethnopharmacol 2008;118(3):361-6.

IssueVol 50, No 9 (2012) QRcode
Elaeagnus Angustifolia Histology Hydroxyproline Rat Wound healing

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Mehrabani Natanzi M, Pasalar P, Kamalinejad M, Dehpour AR, Tavangar SM, Sharifi R, Ghanadian N, Rahimi-Balaei M, Gerayesh-Nejad S. Effect of Aqueous Extract of Elaeagnus angustifolia Fruit on Experimental Cutaneous Wound Healing in Rats. Acta Med Iran. 50(9):589-596.