A Comparative Study of Blood Culture Sampling from Umbilical Catheter Line versus Peripheral Site


Neonatal sepsis is an important cause of death and morbidity in newborns and is diagnosed by isolation of organism in blood culture. In several reports,reliablity of blood cultures were done from umbi lical catheters,have been demonstrated. The objective of the present study was to determine,wether an inde welling umbilical catheter, could be an alternative site for blood culture. In a prospective study over 6 months during 2006,141 paired blood cultures from 134 infant,were done simultaneously from peripheral site and umbilical catheter (mostly U. V. C),during the first four days of life. Majority of these infants were preterm and admitted to NICU for special care. these infants had indwelling umbilical line and had indication of sepsis workup. A total of 141 pairs of blood cultures were obtained from 134 infants. In 16 infants blood culture pairs were positive for one organism in both peripheral vein and umbilical site. 71. 6% of total cultures (n=11)pairs were negative in boths site. A total of 22 pairs were positive in one site only,with 5 positive from peripheral vein only and the other 17 from umblical site. Two pairs were positve in boths site with two different organism. In over all 16 infant (11%)of blood were considered to be contaminated. Contamination rate were 2. 4% and 9. 2% for peripheral and umbilical catheter site. Contamination rate increased after 48 hours of age in umbilical catheter. The result showed that after 2 days contamination rate for blood culture taken from catheter line increased and specifity decreased. We recommended that blood culture via umblical catheter in first 2 days in sick neonates with indwelling catheter can be a alternate site of blood culture sampelling.

Fernandes ACS, de Sousa Junior FC, de Oliveira SM, Calich L, Milan EP. Prevalence of candida sepsis in umbilical catheters implanted in newborns in Natal, Brazil. Braz J Microbiol 2007;38(1):101-34.

Rowen JL, Tate JM, Nordoff N, Passarell L, McGinnis MR. Candida isolates from neonates: frequency of misidentification and reduced fluconazole susceptibility. J Clin Microbiol 1999;37(11):3735-7.

Mermel LA. Prevention of intravascular catheter-related infections. Ann Intern Med 2000;132(5):391-402.

Hines AJ, Rawlins PV. Staphylococcus aureus Septicemia with a fatal transmural myocardial infarction in a 27-weekgestationntwin infant: a case study. Neonatal Netw 2010;29(2):75-85.

Salzman MB, Isenberg HD, Shapiro JF, Lipsitz PJ, Rubin LG. A prospective study of the catheter hub as the portal of entry for microorganisms causing catheter-related sepsis in neonates. J Infect Dis 1993;167(2):487-90.

Cowett RM, Peter G, Hakanson DO, Oh W. Reliability of bacterial culture of blood obtained from an umbilical artery catheter. J Pediatr 1976;88(6):1035-6.

Pourcyrous M, Korones SB, Bada HS, Patterson T, Baselski V. Indwelling umbilical arterial catheter: a preferred sampling site for blood culture. Pediatrics 1988;81(6):821-5.

Bengtsson BS. Coagulase negative staphylococcal sepsis with upper extremity gangrene and shock in a premature infant: An unusual complicatiopn of a peripheralintravenous catheter. Internet J Pediatr Neonatal 2005:5(1); Available from: xmlPrinter=true&xmlFilePath=journals/ijpn/vol5n1/gangre e.xml.

IssueVol 48, No 4 (2010) QRcode
Blood culture umblicus indewlling cateter

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Hamedi A, Mamoury GA, Akhlaghi F. A Comparative Study of Blood Culture Sampling from Umbilical Catheter Line versus Peripheral Site. Acta Med Iran. 1;48(4):231-233.