Remyelination of the Corpus Callosum by Olfactory Ensheathing Cell in an Experimental Model of Multiple Sclerosis

  • Mohammad Azimi Alamouti Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Mehrdad Bakhtiyari Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Fatemeh Moradi Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
  • Tahmineh Mokhtari Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Azim Hedayatpour Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Fariba Zafari Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
  • Mohammad Barbarestani Mail Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Keywords:
Olfactory ensheathing Cell, Remyelination, Demyelination

Abstract

Multiple Sclerosis (MS) causes loss of the myelin sheath, which leads to loss of neurons. Regeneration of myelin sheath stimulates axon regeneration and neurons’ survival. In this study, olfactory ensheathing cell (OEC) transplantation is investigated to restore myelin sheath in an experimental model of MS in male mice.OECs were isolated from the olfactory mucosa of seven-day-old infant rats and cultured. Then, cells were evaluated and approved by flow cytometry by p75 and GFAP markers. A total of 32 mice (C57BL /6) were studied in four groups; 1) without any treatment (control), 2) Sham (receiving PBS), 3) MS model and 4) MS and OEC transplantation. MS was induced by adding Cuprizon in the diet of animals for six weeks. After the expiration of 20 days, histologic analysis was performed with approval of the presence of cells in the graft area and the removal of myelin and myelin regeneration with two types of luxal fast blue (LFB) staining and immunohistochemistry. The purity of the cells ensheathing the olfactory was 90%.  There was a significant difference in Myelin percentage of PBS and OEC recipient groups (P≤0.05). MBP and PLP of the myelin sheath in the group receiving OECs were more than MS group.According to the findings, in MS model MBP and PLP of the myelin sheath is reduced. In the group receiving OECs, it was returned to a normal level significantly compared to the sham group received only PBS significant differences were observed. The OECs transplantation can improve myelin restoration.

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Published
2015-10-06
How to Cite
1.
Azimi Alamouti M, Bakhtiyari M, Moradi F, Mokhtari T, Hedayatpour A, Zafari F, Barbarestani M. Remyelination of the Corpus Callosum by Olfactory Ensheathing Cell in an Experimental Model of Multiple Sclerosis. Acta Med Iran. 53(9):533-539.
Section
Original Article(s)