Vaccination of Diffuse Large B- Cell Lymphoma Patients with Antigen-Primed Dendritic Cells

  • Saeid Abediankenari Mail Department of Immunology, Immonogenetic Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Ghasem Janbabaei Mollae Department of Hematology and Oncology, Mazandaran University of Medical Sciences, Sari, Iran.
  • Maryam Ghasemi Department of Pathology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Yousef Yousefzadeh Department of Immunology, Immonogenetic Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  • Masoud Bahrami Department of Orthopedic, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
  • Kamran Alimoghaddam Department of Hematology and Oncology, Transplantation Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Dendritic cell, Lymphoma, Vaccine


Dendritic cells (DCs) are professional antigen presenting cells that have a potential role in the initiating of immune responses. The cell vaccination is a new strategy in treatment of infectious diseases and cancers. In this study, we have generated monocyte-derived dendritic cells of lymphoma patient's peripheral blood mononuclear cells then; these cells were used as vaccine in lymphoma patients. We generated dendritic cell vaccine from lymphoma patient's blood monocytes with human interleukin-4, granulocyte monocyte colony stimulating factor and then, antigen-primed Dcs were administrated subcutaneously close to the inguinal lymph nodes after maturation of dendritic cells. After 7 days, we analyzed immune response in lymphoma patients with determining of LDH, Beta 2 Microglobulin, CD4+T cell percent, CD8+ Tcell percent and Tumor size before and after vaccination. Furthermore, phenotypic and functional analysis of dendritic cells was performed using anti CD83-FITC monoclonal antibodies. Before vaccination, the mean ± SD of LDH was 530.62±140.65 but after vaccination it was 459±109.45 that significantly different between experimental groups (P=0.002). In addition, the CD8+ T cells percentage significantly different between two groups (P=0.002). We concluded that the use of dendritic cell probably is one of the suitable noninvasive treatments for lymphoma patients that they have not response to chemical drugs.


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How to Cite
Abediankenari S, Janbabaei Mollae G, Ghasemi M, Yousefzadeh Y, Bahrami M, Alimoghaddam K. Vaccination of Diffuse Large B- Cell Lymphoma Patients with Antigen-Primed Dendritic Cells. Acta Med Iran. 51(5):284-288.