Evaluation of the Expression of P-Glycoprotein in Propoxur-Resistant Caco-2 Cells

  • Shabnam Yazdian Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  • Najmeh Fahham Department of Pharmacology, Iran Pasteur Institute, Tehran, Iran.
  • Mohammad Hossein Ghahremani Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  • Monireh Afzali Virtual School, Tehran University of Medical Sciences, Tehran, Iran.
  • Narges Farsandaj Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  • Melody Vatankhah Department of Biology, College of Science, University of Tehran, Tehran, Iran.
  • Seyed Nasser Ostad Mail Department of Toxicology and Pharmacology, Toxicology and Poisoning Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Keywords:
Propoxur, Toxicity, P-glycoprotein, Resistant

Abstract

There is a great concern about the effect of propoxur, as one of the more common N-methyl carbamate pesticides, on human health due to its extensive use in agricultural and non-agricultural applications. Caco-2 cells became resistant to propoxur, and the resistance was confirmed through MTT assay. Then the cell membrane integrity and P-glycoprotein expression were measured by LDH assay and western blot analysis, respectively and compared to the parent cells.  Contrary to what was expected, the expression of P-glycoprotein in propoxur resistant cells was lower than parent cells.This study indicates that the resistance to propoxur may not be related to P-glycoprotein expression directly, since P-glycoprotein expression has decreased in these cells.

References

Balimane PV, Chong S, Morrison RA. Current methodologies used for evaluation of intestinal permeability and absorption. J Pharmacol Toxicol Methods 2000;44(1):301-12.

Banerjee BD, Seth V, Bhattacharya A, et al. Biochemical effects of some pesticides on lipid peroxidation and freeradical scavengers. Toxicol Lett 1999;107(1-3):33-47.

Maran E, Fernández M, Barbieri P, et al. Effects of four carbamate compounds on antioxidant parameters. Ecotox Environ Safe 2009;72(3):922-30.

Maran E, Fernández-Franzón M, Font G, et al. Effects of aldicarb and propoxur on cytotoxicity and lipid peroxidation in CHO-K1 cells. Food Chem Toxicol 2010;48(6):1592-6.

Carriere V, Chambaz J, Rousset M. Intestinal responses to xenobiotics. Toxicol In Vitro 2001;15(4-5):373-8.

Seelig A, Landwojtowicz E. Structure-activity relationship of P-glycoprotein substrates and modifiers. Eur J Pharm Sci 2000;12(1):31-40.

Kang W, Weiss M. Influence of P-glycoprotein modulators on cardiac uptake, metabolism, and effects of idarubicin. Pharm Res 2001;18(11):1535-41.

Lanning CL, Fine RL, Sachs CW, et al. Chlorpyrifos oxon interacts with the mammalian multidrug resistance protein, P-glycoprotein. J Toxicol Environ Health 1996;47(4):395-407.

Lecoeur S, Videmann B, Mazallon M. Effect of= organophosphate pesticide diazinon on expression and activity of intestinal P-glycoprotein. Toxicol Lett 2006;161(3):200-9.

Abu-Qare AW, Elmasry E, Abou-Donia MB. A role for Pglycoprotein in environmental toxicology. J Toxicol Environ Health B Crit Rev 2003;6(3):279-88.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65(1):55-63.

Ghahremani MH. Distinct roles for Galphai2, Galphai3, and Gbeta gamma in modulation offorskolin- or Gsmediated cAMP accumulation and calcium mobilization by dopamine D2S receptors. J Biol Chem 1999;274(14):9238-45.

Sambrook J, Gething MJ. Protein structure. Chaperones, paperones. Nature 1989;342(6247):224-5.

Smulders CJ, Bueters TJ, Van Kleef RG, et al. Selective effects of carbamate pesticides on rat neuronal nicotinic acetylcholine receptors and rat brain acetylcholinesterase. Toxicol Appl Pharmacol 2003;193(2):139-46.

Kuo HH, Shyu SS, Wang TC. Genotoxicity of low dose Nnitroso propoxur to human gastric cells. Food Chem Toxicol. 2008;46(5):1619-26.

Habibollahi P, Ghahremani MH, Azizi E, et al. Multi drug resistance-1 (MDR1) expression in response to chronic diazinon exposure: an in vitro study on Caco-2 cells. B Environ Contam Tox 2011;86(1):105-9.

Ostad SN, Maneshi A, Sharifzadeh, et al. Effect of 17-beta Estradiol on the Expression of Inducible Nitric Oxide Synthase in Parent and Tamoxifen Resistant T47D Breast Cancer Cells. Iranian J Pharmaceutic Res 2009;2(8):125-33.

Fouladdel S, Azizi E, Rahimi HR, et al. Determination of the Expression of COX II and Aromatase Protein in Parent and Tamoxifen-resistant Subline of Human Breast Cancer T47D cells. Med Oncol 2011;6(7):710-5.

Soloneski S, Reigosa MA, Molinari G, et al. Genotoxic and cytotoxic effects of carbofuran and furadan on Chinese hamster ovary (CHOK1) cells. Mutat Res 2008;656(1-2):68-73.

Cavret S, Videmann B, Mazallon M, et al. Diazinon cytotoxicity and transfer in Caco-2 cells: effect of longterm exposure to the pesticide. Environ Toxicol Pharmacol 2005;20(2):375-80.

Limtrakul P, Khantamat O, Pintha K. Inhibition of Pglycoprotein function and expression by kaempferol and quercetin. J Chemother 2005;17(1):86-95.

Anderle P, Niederer E, Rubas W, et al. P-glycoprotein (Pgp) mediated efflux in Caco2 cell monolayers: the influence of culturing conditions and drug exposure on Pgp expression levels. J Pharm Sci 1998;87(6):757-62.

How to Cite
1.
Yazdian S, Fahham N, Ghahremani MH, Afzali M, Farsandaj N, Vatankhah M, Ostad SN. Evaluation of the Expression of P-Glycoprotein in Propoxur-Resistant Caco-2 Cells. Acta Med Iran. 52(10):728-733.
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