Evaluation of the Expression of P-Glycoprotein in Propoxur-Resistant Caco-2 Cells

  • Shabnam Yazdian Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  • Najmeh Fahham Department of Pharmacology, Iran Pasteur Institute, Tehran, Iran.
  • Mohammad Hossein Ghahremani Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  • Monireh Afzali Virtual School, Tehran University of Medical Sciences, Tehran, Iran.
  • Narges Farsandaj Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  • Melody Vatankhah Department of Biology, College of Science, University of Tehran, Tehran, Iran.
  • Seyed Nasser Ostad Mail Department of Toxicology and Pharmacology, Toxicology and Poisoning Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Propoxur, Toxicity, P-glycoprotein, Resistant


There is a great concern about the effect of propoxur, as one of the more common N-methyl carbamate pesticides, on human health due to its extensive use in agricultural and non-agricultural applications. Caco-2 cells became resistant to propoxur, and the resistance was confirmed through MTT assay. Then the cell membrane integrity and P-glycoprotein expression were measured by LDH assay and western blot analysis, respectively and compared to the parent cells.  Contrary to what was expected, the expression of P-glycoprotein in propoxur resistant cells was lower than parent cells.This study indicates that the resistance to propoxur may not be related to P-glycoprotein expression directly, since P-glycoprotein expression has decreased in these cells.


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How to Cite
Yazdian S, Fahham N, Ghahremani MH, Afzali M, Farsandaj N, Vatankhah M, Ostad SN. Evaluation of the Expression of P-Glycoprotein in Propoxur-Resistant Caco-2 Cells. Acta Med Iran. 52(10):728-733.