Evaluation of the Expression of P-Glycoprotein in Propoxur-Resistant Caco-2 Cells
-
Shabnam Yazdian
,
Najmeh Fahham
,
Mohammad Hossein Ghahremani
,
Monireh Afzali
,
Narges Farsandaj
,
Melody Vatankhah
,
Seyed Nasser Ostad
Abstract
There is a great concern about the effect of propoxur, as one of the more common N-methyl carbamate pesticides, on human health due to its extensive use in agricultural and non-agricultural applications. Caco-2 cells became resistant to propoxur, and the resistance was confirmed through MTT assay. Then the cell membrane integrity and P-glycoprotein expression were measured by LDH assay and western blot analysis, respectively and compared to the parent cells. Contrary to what was expected, the expression of P-glycoprotein in propoxur resistant cells was lower than parent cells.This study indicates that the resistance to propoxur may not be related to P-glycoprotein expression directly, since P-glycoprotein expression has decreased in these cells.
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| Issue | Vol 52, No 10 (2014) | |
| Section | Original Article(s) | |
| Keywords | ||
| Propoxur Toxicity P-glycoprotein Resistant | ||
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