Investigating the Effect of Phlomis Lanceolata Boiss and Hohen on Cancer Cell Lines

  • Farnaz Soltani-Nasab Department of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
  • Jinous Asgarpanah Department of Pharmacognosy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
  • Maryam Majdzadeh Department of Toxicology and Pharmacology, Faculty of Pharmacy, and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran AND Virtual School, Tehran University of Medical Sciences, Tehran, Iran.
  • Seyed Nasser Ostad Mail Department of Toxicology, Pharmacology and Nanotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Keywords:
Cell line, Cytotoxic activity, MTT assay, Phlomis lanceolata

Abstract

Phlomis lanceolata is a medicinal plant that has long been used to treat various conditions such as diabetes, gastric ulcer, hemorrhoids, inflammation and wounds. As most of Phlomis species have shown cytotoxic activity against proliferation of different cell lines, a biological investigation of P. lanceolata was carried out in this study. The aim of this study was to find out the in vitro cytotoxic activity of total extract and different fractions of Phlomis lanceolata on four cell lines. Cytotoxic activity of the metanolic total extract and partition fractions of chloroform, ethyl acetate and petroleum ether of flowering aerial parts of Phlomis lanceolata on the HT29, Caco2, T47D and NIH3T3 cell lines is examined by MTT. Petroleum ether fraction showed high cytotoxic activity against proliferation of all four cell lines. Presence of heavy triterpens and lipophil compounds recognized by TLC test in Petroleum ether fraction is responsible for high cytotoxic activity. The results emphasize the importance of phytochemical studies which could lead to the discovery of new active compounds.

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How to Cite
1.
Soltani-Nasab F, Asgarpanah J, Majdzadeh M, Ostad SN. Investigating the Effect of Phlomis Lanceolata Boiss and Hohen on Cancer Cell Lines. Acta Med Iran. 52(5):333-336.
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