The Effect of Gamma Irradiation on the Osteoinductivity of Demineralized Human Bone Allograft

  • Babak Arjmand Chronic Diseases Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran. AND Brain and Spinal Cord Injury Research Center, Tehran University of Medical Sciences, Tehran, Iran.
  • Hamid Reza Aghayan Chronic Diseases Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran. AND Brain and Spinal Cord Injury Research Center, Tehran University of Medical Sciences, Tehran, Iran.
  • Bagher Larijani Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
  • Mehrnaz Sahebjam Brain and Spinal Cord Injury Research Center, Tehran University of Medical Sciences, Tehran, Iran.
  • Firoozeh Ghaderi Brain and Spinal Cord Injury Research Center, Tehran University of Medical Sciences, Tehran, Iran.
  • Parisa Goodarzi Mail Brain and Spinal Cord Injury Research Center, Tehran University of Medical Sciences, Tehran, Iran. AND Cellul Fanavaran Knowledge-Based Organization, Tehran University of Medical Sciences, Tehran, Iran.
Keywords:
Gamma irradiation, Bone allograft, Osteoinduction, Osteoconduction, Sterilization

Abstract

The gamma irradiation has been used for end sterilization of allograft bones and its effects with a 25 kGy dosage on the osteoinductive properties of demineralized bone allograft powder was studied. This work carried out using an experimental method in an animal model. In this study the demineralized bone allograft powder which had been sterilized and prepared with gamma irradiation in a 25 kGy dosage in 18 hours, was used as a study group and the demineralized bone allograft powder which had been prepared aseptically was used as the reference group. 30 mg of bone powder from each group were implanted into right and left paravertebral muscles of eighteen rats, separately. After four weeks, the implanted samples were harvested with a 0.5 cm border and then the osteoinductivity of implants in two groups were compared with histopathologic studies. In 94.4% of the reference samples a new bone formation was observed. In the study group, this difference was observed only in 27.7% of samples (P<0.002). It appears that using gamma irradiation may lead to a reduction in osteoinduction properties of demineralized bone allograft powder.

References

Tomford WW. Bone allograft:past, present and future. Cell Tissue Bank 2000;1(2):105-9.

Tomford WW. Transmission of disease through transplantation of musculoskeletal allografts. J Bone JointSurg Am 1995;77(11):1742-54.

Sanzen L, Carlsson A. Transmission of human T celllymphotropic virus type 1 by a deep-frozen bone allograft.Acta Orthop Scand 1997;68(1):72-4.

Cook Sd, Salkeld SL, Prewett AB. Simian immunodeficiency virus (human HIV-II) transmission in allograft bone procedures. Spine 1995;20(12):1338-42.

Conrad EU, Gretch DR, Obermeyer KR, et al. Transmission of the Hepatitis-C virus by tissue transplantation. J Bone Joint Surg Am 1995;77(2):214-24.

Hilmy N, Lina M. Effect of ionising radiation on viruses, proteins and prions. In:Nather A, editor. Advances in Tissue Banking. The Scientific Basis of Tissue Transplantation. 5th ed. Singapore: World Scientific Publication Co. Pte. Ltd.; 2001; p. 358-74.

Glowacki J. A review of osteoinductive testing methodsand sterilization process for demineralized bone. Cell Tissue Bank 2005;6(1):3-12.

Radiation Sterilization of Tissue Allografts: Requirements for Validation and Routine Control, A Code of Practice, International Atomic Energy Agency. (Accessed in Jan 2014, 28, at http://www.iaea.org).

General standards for tissue banking. European Association of Tissue Banks (EATB). (Accessed in Jan 2014, 28, at http://eatb.org).

Standards for tissue banking. American Association of Tissue Banks (AATB). (Accessed in Jan 2014, 28, at http://aatb.org).

Dziedzic-Goclawska A, Kaminiski A, et al. Irradiation as a safety procedure in tissue banking. Cell Tissue Bank 2005;6(3):201-19.

Glowacki J, Mulliken JB. Demineralized bone implants. Clin Plas Surg 1985;12(2):233-241.

Urist MR, Hernandez A. Excitation transfer in bone. Deleterious effects of cobalt 60 radiation-sterilization of bank bone. Arch Surg 1974;109(4):586-93

Glowacki J, Kaban LB, Murray JE, et al. Application of the biological principle of induced osteogenesis for craniofacial defects. Lancet 1981;1(8227):959-62.

Dzeidzic-Goclawski A, Ostrowski K, Stachowics W, et al. Effect of radiation sterilization on the osteoinductive properties and the rate of remodeling of bone implants preserved by lyophilization and deep freezing. Clin Orthop Relat Res 1991;272(1):30-7.

Akkus O, Rimnac CM. Fracture resistanc of gamma radiation sterilized cortical bone allografts. J Orthop Res 2001;19(5):927-934.

Zhou Z, Qin T, Yang J, et al. Mechanical strength of cortical allografts with gamma radiation versus ethylene oxide sterilization. Acta Orthop Belg 2011;77(5):670-5.

How to Cite
1.
Arjmand B, Aghayan HR, Larijani B, Sahebjam M, Ghaderi F, Goodarzi P. The Effect of Gamma Irradiation on the Osteoinductivity of Demineralized Human Bone Allograft. Acta Med Iran. 52(3):215-219.
QRcode
Section
Articles