Possible Role for Growth Hormone in Suppressing Acylated Ghrelin and Hunger Ratings During and After Intermittent Exercise of Different Intensities in Obese Individuals

  • Majid Gholipour Mail Physical Education Center, Sharif University of Technology, Tehran, Iran.
  • Mohamad Reza Kordi Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
  • Mohamad Taghikhani Department of Clinical Biochemistry, Faculty of Medicine Sciences, Tarbiat Modares University, Tehran, Iran.
  • Ali Asghar Ravasi Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
  • Abas Ali Gaeini Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
  • Arezoo Tabrizi Physical Education Center, Sharif University of Technology, Tehran, Iran.
Acylated ghrelin, Appetite, College students, Obesity, Running


Body weight is influenced by both food intake and energy expenditure. Acylated ghrelin enhances appetite, and its circulating level is suppressed by growth hormone. Data on the acylated ghrelin responses to exercise of different intensities in obese individuals are currently not available. This study examined the effects of an intermittent exercise protocol on acylated ghrelin levels and hunger ratings in obese people. Nine inactive male ran on the treadmill at 0900 with progressive intensities of 50, 60, 70, and 80% of VO2max for 10, 10, 5, and 2 min respectively. Blood samples were collected before the exercise at 0845 (-15 min as the resting values), after each workload (10, 23, 31, and 36 min during exercise), and at 30, 60, and 120 min thereafter. The control trial was conducted under identical conditions with the exception of exercise. Compared to the baseline, both acylated ghrelin levels and hunger ratings were suppressed at 70% of VO2max during exercise (17.74 vs. 9.80 pmol/L and 4.84 vs. 2.96 unit respectively) and remained significantly lower than the control trial 2 h after the cessation of exercise (13.95 vs. 20.32 pmol/L and 3.33 vs. 6.04 unit, respectively). Growth Hormone increased during the exercise period and peaked at 80% of VO2max. These findings indicate that acylated ghrelin concentrations and hunger ratings are suppressed during exercise and two hours thereafter in obese individuals, and it is possible that Growth Hormone caused the suppression of acylated ghrelin.


Stiegler P, Cunliffe A. The Role of Diet and Exercise for the Maintenance of Fat-Free Mass and Resting Metabolic Rate during Weight Loss. Sports Med 2006;36(3):239-62.

Frayn KN. Metabolic Regulation: A Human Perspective. 2nd ed. Wiley-Blackwell 2003.

Ariyasu H, Takaya K, Tagami T, Ogawa Y, Hosoda K, Akamizu T, Suda M, Koh T, Natsui K, Toyooka S, Shirakami G, Usui T, Shimatsu A, Doi K, Hosoda H, Kojima M, Kangawa K, Nakao K. Stomach Is a Major Source of Circulating Ghrelin, and Feeding State Determines Plasma Ghrelin-Like Immunoreactivity Levels in Humans. J Clin Endocrinol Metab 2001,86(10):4753-8.

Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K. Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature 1999;402(6762):656-60.

Murphy KG, Bloom SR. Gut hormones and the regulation of energy homeostasis. Nature 2006,14;444(7121):854-9.

Drazen DL, Woods SC. Peripheral signals in the control of satiety and hunger. Curr Opin Clin Nutr Metab Care 2003;6(6):621-9.

Broglio F, Benso A, Gottero C, Prodam F, Gauna C, Filtri L, Arvat E, van der Lely AJ, Deghenghi R, Ghigo E. Nonacylated ghrelin does not possess the pituitaric and pancreatic endocrine activity of acylated ghrelin in humans. J Endocrinol Invest 2003;26(3):192-6.

Tschöp M, Weyer C, Tataranni PA, Devanarayan V, Ravussin E, Heiman ML. Circulating Ghrelin Levels Are Decreased in Human Obesity. Diabetes 2001;50(4):707-9.

Soriano-Guillén L, Barrios V, Campos-Barros A, Argente J. Ghrelin levels in obesity and anorexia nervosa: effect of weight reduction or recuperation. J Pediatr 2004;144(1):36-42.

Jakicic JM, Clark K, Coleman E, Donnelly JE, Foreyt J, Melanson E, Volek J, Volpe SL; American College of Sports Medicine. Appropriate Intervention Strategies for Weight Loss and Prevention of Weight Regain for Adults. Med Sci Sports Exerc 2001;33(12):2145-56.

Blundell JE, King NA. Physical activity and regulation of food intake: current evidence. Med Sci Sports Exerc 1999;31(11 Suppl):573-83.

Burns SF, Broom DR, Miyashita M, Mundy C, Stensel DJ. A single session of treadmill running has no effect on plasma total ghrelin concentrations. J Sports Sci 2007;25(6):635-42.

Jürimäe J, Hofmann P, Jürimäe T, Palm R, Mäestu J, Purge P, Sudi K, Rom K, von Duvillard SP. Plasma ghrelin responses to acute sculling exercises in elite male rowers. Eur J Appl Physiol 2007;99(5):467-74.

Kraemer RR, Durand RJ, Acevedo EO, Johnson LG, Kraemer GR, Hebert EP, Castracane VD. Rigorous Running Increases Growth Hormone and Insulin-Like Growth Factor-I Without Altering Ghrelin. Exp Biol Med 2004;229(3):240-6.

Schmidt A, Maier C, Schaller G, Nowotny P, Bayerle-Eder M, Buranyi B, Luger A, Wolzt M. Acute exercise has no effect on ghrelin plasma concentrations. Horm Metab Res 2004;36(3):174-7.

Broom DR, Batterham RL, King JA, Stensel DJ. Influence of resistance and aerobic exercise on hunger, circulating= levels of acylated ghrelin, and peptide YY in healthy males. Am J Physiol Regul Integr Comp Physiol 2009;296(1):29-35.

Broom DR, Stensel DJ, Bishop NC, Burns SF, MiyashitaM. Exercise-induced suppression of acylated ghrelin in humans. J Appl Physiol 2007;102(6):2165-71.

King JA, Wasse LK, Ewens J, Crystallis K, Emmanuel J, Batterham RL, Stensel DJ. Differential acylated ghrelin, peptide YY3-36, appetite and food intake responses to equivalent energy deficits created by exercise andfood restriction. J Clin Endocrinol Metab 2011;96(4):1114-21.

King JA, Miyashita M, Wasse LK, Stensel DJ. Influence of prolonged treadmill running on appetite, energy intake and circulating concentrations of acylated ghrelin. Appetite 2010;54(3):492-8.

Chang FE, Dodds WG, Sullivan M, Kim MH,Malarkey WB. The acute effects of exercise on prolactin and growth hormone secretion: comparison between sedentary women and women runners with normal and abnormal menstrual cycles. J Clin Endocrinol Metab 1986;62(3):551-6.

Felsing NE, Brasel JA, Cooper DM. Effect of low and high intensity exercise on circulating growth hormone in men. J Clin Endocrinol Metab 1992;75(1):157-62.

Gilbert KL, Stokes KA, Hall GM, Thompson D. Growth hormone responses to 3 different exercise bouts in 18- to

- and 40 - to 50-year-old men. Appl Physiol Nutr Metab 2008;33(4):706-12.

Pritzlaff CJ, Wideman L, Weltman JY, Abbott RD, Gutgesell ME, Hartman ML, Veldhuis JD, Weltman A. Impact of acute exercise intensity on pulsatile growth hormone release in men. J Appl Physiol 1985;87(2),498-504.

Vestergaard ET, Dall R, Lange KH, Kjaer M, Christiansen JS, Jorgensen JO. The Ghrelin Response to Exercise before and after Growth Hormone Administration. J Clin Endocrinol Metab 2006; 92(1):297-303.

Jackson AS, Pollock ML. Generalized equations for predicting body density of men. Br J Nutr 1978;40(3):497-504.

Dill DB, Costill DL. Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration. J Appl Physiol 1974;37(2):247-8.

Flint A, Raben A, Blundell JE, Astrup A. Reproducibility, power and validity of visual analogue scales in assessment of appetite sensations in single test meal studies. Int J Obes Relat Metab Disord 2000;24(1):38-48.

Stock S, Leichner P, Wong AC, Ghatei MA, Kieffer TJ, Bloom SR, Chanoine JP. Ghrelin, peptide YY, glucosedependent insulinotropic polypeptide, and hunger responses to a mixed meal in anorexic, obese, and control female adolescents. J Clin Endocrinol Metab 2005;90(4):2161-8.

Christ ER, Zehnder M, Boesch C, Trepp R, Mullis PE, Diem P, Décombaz J. The effect of increased lipid intake on hormonal responses during aerobic exercise in endurance-trained men. Eur J Endocrinol 2006;154(3):397-403.

Mackelvie KJ, Meneilly GS, Elahi D, Wong AC, Barr SI, Chanoine JP. Regulation of appetite in lean and obese adolescents after exercise: role of acylated and desacyl ghrelin. J Clin Endocrinol Metab 2007;92(2):648-54.

Asanoi H, Wada O, Miyagi K, Ishizaka S, Kameyama T, Seto H, Sasayama S. New Redistribution Index of Nutritive Blood Flow to Skeletal Muscle During Dynamic Exercise. Circulation 1992;85(4),1457-63.

Rowell LB. Human cardiovascular adjustments to exercise and thermal stress. Physiol Rev 1974;54(1):75-159.

Iranmanesh A, Lizarralde G, Veldhuis JD. Age and Relative Adiposity Are Specific Negative Determinants of the Frequency and Amplitude of Growth Hormone (GH) Secretory Bursts and the Half-Life of Endogenous GH in Healthy Men. J Clin Endocrinol Metab 1991;73(5):1081-8.

Scacchi M, Pincelli AI, Cavagnini F. Growth hormone in obesity. Int J Obes Relat Metab Disord 1999;23(3):260-71.

How to Cite
Gholipour M, Kordi MR, Taghikhani M, Ravasi AA, Gaeini AA, Tabrizi A. Possible Role for Growth Hormone in Suppressing Acylated Ghrelin and Hunger Ratings During and After Intermittent Exercise of Different Intensities in Obese Individuals. Acta Med Iran. 52(1):29-37.