Acceleration of skin wound healing with tragacanth (Astragalus) preparation: an experimental pilot study in rats.
AbstractGum tragacanth is a natural complex mixture of polysaccharides and alkaline minerals extracted from species of Astragalus plant, which is found widely in arid regions of the Middle East. In a pilot experimental study we examined the effects of its topical application on wound healing in ten albino adult male rats. Two similar parasagittal elliptical full-thickness wounds (control vs. test samples) were created on the dorsum of each animal. Test group samples were fully covered by a thin layer of gum tragacanth daily. The extent of wound healing was evaluated by planimetric analysis on multiple occasions during the 10-day study period. On the 7th day of the study, the percent of wound closure was significantly higher in gum tragacanth-treated specimens compared to the control samples (87%±2% vs. 70%±4%, P<0.001). The majority of wounds in the test group were completely closed by the 10th day of the study. The difference in wound healing index measured by histological examination on day 10 of the study was also statistically meaningful between the two groups (0.624±0.097 vs. 0.255±0.063, P<0.05). The results of this study clearly showed the useful effects of topical application of gum tragacanth in acceleration of skin wound contraction and healing. More studies are encouraged to identify the implicating agents and precisely understand the mechanism by which they exert their wound healing effects.
Winter GD. Formation of the scab and the rate of epithelialization of superficial wounds in the skin of the young domestic pig. Nature 1962;193(4812):293-4.
Purna SK, Babu M. Collagen based dressings-a review. Burns 2000;26(1):54-62.
Ishihara M, Nakanishi K, Ono K, et al. Photocrosslinkable chitosan as a dressing for wound occlusion and accelerator in healing process. Biomaterials 2002;23(3):833-40.
Ishihara M, Ono K, Sato M, et al. Acceleration of woundcontraction and healing with a photocrosslinkable chitosan hydrogel. Wound Repair Regen 2001;9(6):513-21.
Thomas S. Alginate dressings in surgery and wound management: -- Parts 1. J Wound Care 2000;9(2):56-60.
Peplow PV. Glycosaminoglycan:a candidate to stimulate the repair of chronic wounds. Thromb Haemost 2005;94(1):4-16.
Gentry HS. Gum tragacanth in Iran. Econ Bot 1957;11(1):40-63.
Burdock GA, editor. Encyclopedia of food and color additives: . 1st ed. Boca Raton:CRC Press; 1996: p. 2818-20.
Iwu MM, editor. Handbook of African medicinal plants. 1st ed. Boca Raton:CRC Press;1993:p. 123-4.
Weiping W. Tragacanth and karaya. In:Phillips GO, Williams PA, editors. Handbook of hydrocolloids. 1st ed. Boca Raton:CRC Press;2000:p. 231-46.
Al-Hazmi MI, Stauffer KR, editors. Gas chromatographic determination of hydrolyzed sugars in commercial gums. J Food Sci 1986;51:1091-2.
Whistler RL, BeMiller JN, editors. Industrial gums:polysaccharides and their derivatives. 1st ed. New York:Academic Press;1993:p. 289-99.
Davidson RL. Handbook of water soluble gums and resins. Illustrated ed. New York:McGraw-Hill;1980:p. 1-11.
Elvers B, Hawkins S, Russey W, editors. Ullmann’s encyclopedia of industrial chemistry:Volume A25. 1 sted. Weinheim:VCH;1994:p. 1-62.
Amer S, Kamil R, Siddiqui PQ. The hypolipidaemic effect of gum tragacanth in diet induced hyperlipidaemia in rats. Pak J Pharm Sci 1999;12(2):33-9.
Eastwood MA, Brydon WG, Anderson DM. The effects of dietary gum tragacanth in man. Toxicol Lett 1984;21(1):73-81.
Hagiwara A, Tanaka H, Tiwawech D, et al. Oral toxicity study of tragacanth gum in B6C3F1 mice:development of squamous-cell hyperplasia in the forestomach and its reversibility. J Toxicol Environ Health 1991;34(2):207-18.
Hagiwara A, Boonyaphiphat P, Kawabe M, et al. Lack of carcinogenicity of tragacanth gum in B6C3F1 mice. Food Chem Toxicol 1992;30(8):673-9.
Strobel S, Ferguson A, Anderson DM. Immunogenicity, immunological cross reactivity and non-specific irritant properties of the exudate gums, arabic, karaya and tragacanth. Food Addit Contam 1986;3(1):47-56.
Moghbel A, Hemmati AA, Agheli H, et al. The effect of tragacanth mucilage on the healing of full-thickness wound in rabbit. Arch Iranian Med 2005;8(4):257-62.
Kanani AG, Bahrami SH, Ahmadi Tafti SH, et al. Effect of chitosan-polyvinyl alcohol blend nanofibrous web on the healing of excision and incision full thickness wounds. IET Nanobiotechnol 2010;4(4):109-17.
Ueno H, Yamada H, Tanaka I, et al. Accelerating effects of chitosan for healing at early phase of experimental open wound in dogs. Biomaterials 1999;20(15):1407-14.
VandeVord PJ, Matthew HW, DeSilva SP, et al. Evaluation of the biocompatibility of a chitosan scaffold in mice. J Biomed Mater Res 2002;59(3):585-90.
Usami Y, Minami S, Okamoto Y, et al. Influence of chain length of N-acetyl-D-glucosamine and D-glucosamine residues on direct and complement-mediated chemotactic activities for canine polymorphonuclear cells. Carbohydrate Polymers 1997;32(2):115-22.
Peluso G, Petillo O, Ranieri M, et al. Chitosan-mediated stimulation of macrophage function. Biomaterials 1994;15(15):1215-20.
Masuoka K, Ishihara M, Asazuma T, et al. The interaction of chitosan with fibroblast growth factor-2 and its protection from inactivation. Biomaterials 2005;26(16):3277-84.
Nergard CS, Matsumoto T, Inngjerdingen M, et al. Structural and immunological studies of a pectin and a pectic arabinogalactan from Vernonia kotschyana Sch. Bip. Ex Walp. (Asteraceae). Carbohydr Res 2005;340(1):115-30.
Yamada H, Kiyohara H. Complement-activating polysaccharides from medicinal herbs. In:Wagner H, editors. Immunomodulatory agents from plants. 1st ed. Basel: Birkhauser;1999:p. 161-202.
Zippel J, Deters A, Hensel A. Arabinogalactans from Mimosa tenuiflora (Willd.) Poiret bark as active principles for wound-healing properties:specific enhancement of dermal fibroblast activity and minor influence on HaCaT keratinocytes. J Ethnopharmacol 2009;124(3):391-6.
Lansdown AB, Sampson B, Rowe A. Sequential changes in trace metal, metallothionein and calmodulin concentrations in healing skin wounds. J Anat (London) 1999;195(Pt 3):375-86.
Lansdown AB. Calcium:a potential central regulator in wound healing in the skin. Wound Rep Reg 2002;10(5):271-85.
Grzesiak JJ, Pierschbacher MD. Shifts in the concentrations of magnesium and calcium in early porcine and rabbit wound fluids activate the cell migratory response. J Clin Invest 1995;95(1):227-33.