Protective Effects of Crocin Against Streptozotocin-Induced Oxidative Damage in Rat Striatum
,
Abstract
The study aimed to investigate the protective effects of crocin (Cro) against streptozotocin (STZ)-induced oxidative damage in rat striatum. Animals were randomly divided into four groups (five each). Group 1 (sham) were treated with normal saline (2 ml/kg, p.o.). Group 2 (STZ-lesioned or lesion) were injected with ICV-STZ (3 mg/kg bilaterally, on day 1 and 3) and treated with normal saline (2 ml/kg, p.o.) respectively, for 21 days. Group 3 (sham+Cro) were injected ICV on day 1 and 3 with artificial CSF and treated with crocin (100 mg/kg, p.o.) for 21 days. Group 4 (lesion+Cro) were injected with ICV STZ (3 mg/kg bilaterally, on day 1 and 3) and treated with crocin (100 mg/kg, p.o.) for 21 days. The homogenized striatum was used for measuring malondialdehyde (MDA), and total thiol contents besides glutathione peroxidase (GPx) activity. Crocin treatment resulted in a significant reduction in MDA concentration as compared to the STZ-lesioned rats. Moreover, crocin produced a significant elevation in total thiol content and GPx activity, as compared with STZ-lesioned group. The present findings provide evidence that crocin may have a therapeutic significance for neurodegenerative diseases such as Alzheimer's disease (AD).
Sharma M, Gupta YK. Chronic treatment with trans resveratrol prevents intracerebroventricular streptozotocin induced cognitive impairment and oxidative stress in rats. Life Sci 2002;71(21):2489-98.
Tiwari V, Kuhad A, Bishnoi M, et al. Chronic treatment with tocotrienol, an isoform of vitamin E, prevents intracerebroventricular streptozotocin-induced cognitive impairment and oxidative–nitrosative stress in rats. Pharmacol Biochem Behav 2009;93(2):183-9.
Sharma M, Gupta YK. Effect of chronic treatment of melatonin on learning, memory and oxidative deficiencies induced by intracerebroventricular streptozotocin in rats. Pharmacol Biochem Behav 2001;70(2-3):325-31.
Ochiai T, Soeda SH, Ohno S, et al. Crocin prevents the death of PC-12 cells through sphingomyelinase-ceramide signaling by increasing glutathione synthesis. Neurochem Int 2004;44(5):321-30.
Konoshima T, Takasaki M, Tokuda H, et al. Crocin and crocetin derivatives inhibit skin tumour promotion in mice. Phytother Res 1998;12(6):400-4.
Abe K, Saito H. Effects of saffron extract and its constituent crocin on learning behavior and long-term potentiation. Phytother Res 2000;14(3):149-52.
Pitsikas N, Zisopoulou S, Tarantilis PA, et al. Effects of the active constituents of Crocus sativus L., crocins on recognition and spatial rats’ memory. Behav Brain Res 2007;183(2):141-6.
Sugiura M, Shoyama Y, Saito H, e al. The effects of ethanol and crocin on the induction of long-term potentiation in the CA1 region of rat hippocampal slices. Jpn J Pharmacol 1995;67(4):395-7.
Lee IA, Lee JH, Baek NI, et al. Antihyperlipidemic effect of crocin isolated from the fractus of Gardenia jasminoides and its metabolite crocetin. Biol Pharm Bull 2005;28(11):2106-210.
Garcia-Olmo DC, Riese HH, Escribano J, et al. Effects of long-term treatment of colon adenocarcinoma with crocin, a carotenoid from saffron (Crocus sativus L.): an experimental study in the rat. Nutr Cancer 1999;35(2):120-6.
He SHY, Qian ZY, Tang FT, et al. Effects of crocin on experimental atherosclerosis in quails and its mechanisms. Life Sci 2005;77(8):907-21.
Nam KN, Park YM, Jung HJ, et al. Anti-inflammatory effects of crocin and crocetin in rat brain microglial cells. Eur J Pharmacol 2010;648(1-3):110-6.
Zheng YQ, Liu JX, Wan JN, et al. Effects of crocin on reperfusion-induced oxidative/nitrative injury to cerebral microvessels after global cerebral ischemia. Brain Res 2007;1138(1):86-94.
Naghizadeh B, Boroushaki MT, Vahdati Mashhadian N, et al. Protective effects of crocin against cisplatin-induced acute renal failure and oxidative stress in rats. Iran Biomed J 2008;12(2):93-100.
Naghizadeh B, Mansouri MT, Vahdati Mashhadian N. Crocin attenuates cisplatin-induced renal oxidative stress in rats. Food Chem Toxicol 2010;48(10):2650-5.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides inanimal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95(2):351-8.
Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82(1):70-7.
Sharma M, Gupta YK. Intracerebroventricular injection of streptozotocin in rats produces both oxidative stress in the brain and cognitive impairment. Life Sci 2001;68(9):1021-9.
Militao GC, Ferreira PM, de Freitas RM. Effects of lipoic acid on oxidative stress in rat striatum after pilocarpineinduced seizures. Neurochem Int 2010;56(1):16-20.
Rodriguez-Martinez E, Camacho A, Maldonado PD, et al. Effect of quinolinic acid on endogenous antioxidants in rat corpus striatum. Brain Res 2000;858(2):436-9.
Stahl W, Sies H. Antioxidant activity of carotenoids. Mol Aspects Med 2003;24(6):345-51.
| Files | ||
| Issue | Vol 52, No 2 (2014) | |
| Section | Articles | |
| Keywords | ||
| Crocin Streptozotocin Oxidative stress Striatum Rat | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
