The Effect of Vitamin a Supplementation on Disease Progression, Cytokine Levels and Gene Expression in Multiple Sclerotic Patients: Study Protocol For a Randomized Controlled Trial

  • Mohammad Hassan Harrirchian Iranian Center for Neurological Research, Tehran University of Medical Sciences, Tehran, Iran.
  • Niyaz Mohammadzadeh Honarvar Department of Cellular and Molecular Nutrition, Faculty of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
  • Fariba Koohdani Department of Cellular and Molecular Nutrition, Faculty of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
  • Sama Bitarafan Department of Cellular and Molecular Nutrition, Faculty of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
  • Fereydoon Siassi Community Nutrition Department, Faculty of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
  • Sima Jafarirad Department of Cellular and Molecular Nutrition, Faculty of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Nutrition, Faculty of Paramedicine, Jundishapur University of Medical Sciences, Ahvaz, Iran.
  • Mina Abdolahi Department of Cellular and Molecular Nutrition, Faculty of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
  • Eisa Salehi Immunology Department, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Mohammad Ali Saharian Department of Neurology, Sina Hospital, Tehran, Iran.
  • Homa Rouystaee Medicine faculty, Tehran University of Medical Sciences, Tehran, Iran.
  • Mohammad Reza Eshraghian Department of Biostatistics, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  • Ali Akbar Saboor-Yarghi Mail Department of Cellular and Molecular Nutrition, Faculty of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. AND Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Keywords:
Multiple sclerosis, Vitamin A, Magnetic resonance imaging, Cytokine, Gene expression

Abstract

Multiple Sclerosis (MS) is a chronic inflammatory disease that leads to degeneration of the brain and spinal tissue. Imbalances of CD4+ T cells including Thelper1 (Th1)/Thelper2 (Th2) and Thelper17 (Th17)/Tregulatory (Treg), their secreted cytokines and gene expressions, are important aspects of in immunopathogenesis of MS. Vitamin A and its metabolites can regulate the immune system and appears to be effective in preventing progression of the autoimmune disease such as MS. Disease progression was evaluated By Magnetic Resonance Imaging (MRI), Expanded Disability States Scale (EDSS) and Multiple Sclerosis Functional Composite (MSFC) tests. Cytokine levels were measured using ELISA kits and gene expression was quantified by Real time PCR (RT-PCR) system. According to the difference between the epidemiological and clinical data on the relationship between vitamin A and immune system regulation, this study of the first time assesses Immune function as well as gene expression and progression of the disease following administration of vitamin A supplement.

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How to Cite
1.
Harrirchian MH, Mohammadzadeh Honarvar N, Koohdani F, Bitarafan S, Siassi F, Jafarirad S, Abdolahi M, Salehi E, Saharian MA, Rouystaee H, Eshraghian MR, Saboor-Yarghi AA. The Effect of Vitamin a Supplementation on Disease Progression, Cytokine Levels and Gene Expression in Multiple Sclerotic Patients: Study Protocol For a Randomized Controlled Trial. Acta Med Iran. 52(2):94-100.
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