Nasal Carriage of Uncommon Coagulase-Negative Staphylococci in Nurses and Physicians of Tehran University Hospitals
Coagulase-negative staphylococci (CoNS) have been identified as a major cause of nosocomial infections. Nasal carriage of CoNS in nurses and physicians is known to be an important risk factor for potential hospital infections. This study was carried out to investigate the prevalence of nasal carriage of uncommon coagulase-negative staphylococci among nurse and physician staffs of Tehran University Hospitals. A total of 116 CoNS were isolated from anterior nares of the study participants working in different wards of the hospitals. Thirteen uncommon CoNS were identified using phenotypic and biochemical methods, were subsequently confirmed by API kits. Staphylococcus xylosus, Staphylococcus haemolyticus, and Staphylococcus capitis species accounted for 53.85%, 30.77%, and 15.38% from the isolates, respectively. Six isolates (46.15%) were found to be resistant to methicillin. In conclusion, screening of healthcare workers for uncommon CoNS colonization along with identification and testing for susceptibility of cultured isolates is of paramount importance in strengthening effective nosocomial infection control and prevention measures.
Boyce JM. Preventing Staphylococcal Infections by Eradicating Nasal Carriage of Staphylococcus aureus. Infect Control Hosp Epidemiol 1996;17(12):775-9.
Khalili MB, Sharifi-Yazdi MK, Dargahi H, et al. Nasal Colonization rate of Staphylococcus aureus strains among Health Care Service Employee’s of Teaching UniversityHospitals in Yazd. Acta Med Iran 2009;47(4):315-7.
Von-Eiff C, Peters G, Heilmann C. Pathogenesis of infections due to coagulase-negative staphylococci. Lancet Infect Dis 2002;2(11):677-85.
Rastegar-Lari A, Pourmand MR, Ohadian Moghadam S, et al. Prevalence of PVL-containing MRSA isolates among hospital staff nasal carriers. Lab Med 2011;42(5):283-86.
Klingenberg CA, Rønnestad AS, Anderson TG, et al. Persistent strains of coagulase-negative staphylococci in a neonatal intensive care unit: Virulence factors and invasiveness. Clin Microbiol Infect 2007;13(11):1100–11.
Kleeman KT, Bannerman TL, Kloos WE. Species Distribution of Coagulase-Negative StaphylococcalIsolates at a Community Hospital and Implications for Selection of Staphylococcal Identification Procedures. J Clin Microbiol 1993;31(5):1318-21.
Koziol-Montewka M, Szczepanic A, Baranowicz L, et al.The investigation of Staphylococcus aureus and coagulase-negative staphylococci nasal carriage amongpatients undergoing haemodialysis. Microbial Res 2006;161(4):281-7.
Pourmand MR, Abdossamadi Z, Salari MH, et al. Slime layer formation and the prevalence of mecA and aap genes in Staphylococcus epidermidis isolates. J Infect Dev Ctries 2011;5(1):34-40.
Havaei SA, Ohadian Moghadam S, Pourmand MR, et al. Prevalence of genes encoding bi-component leukocidinsamong clinical isolates of methicillin-resistant Staphylococcus aureus. Iranian J Publ Health 2010;39(1):8-14.
Sefani S, Varaldo PE. Epidemiology of methicillinresistant staphylococci in Europe. Clin Microbial Infect2003;9(12):1179-86.
Coia JE, Duckwort GJ, Edwards DI, et al. Guidelines for the control and prevention of methicillin-resistant Staphylococcus aureus (MRSA) in healthcare facilities. J Hosp Infect 2006;63(Suppl 1):S1-44.
Nagase N, Sasaki A, Yamashita K,et al. Isolation andspecies distribution of staphylococci from animal and human skin. J Vet Med Sci 2002;64(3):245-50.
Kloos WE, Musselwhite MS. Distribution and persistenceof Staphylococcus and Micrococcus species and otheraerobic bacteria on human skin. Appl Microbiol1975;30(3):381-5.
Fredheim EG, Klingenberg C, Rohde H, et al. BiofilmFormation by Staphylococcus haemolyticus. J Clin Microbiol 2009;47(4):1172-80.
Froggatt JW, Johnston JL, Galetto DW, et al. Antimicrobial resistance in nosocomial isolates of Staphylococcus haemolyticus. Antimicrob Agents Chemother1989;33(4):460-6 .
Schwalbe RS, Stapleton JT, Gilligan PH. Emergence of vancomycin resistance in coagulase-negative staphylococci. N Engl J Med 1987;316(15):927-31 .
Biavasco F, Vignaroli C, Varaldo PE. Glycopeptide resistance in coagulase-negative staphylococci. Eur J Clin Microbiol Infect Dis 2000;19(6):403-17.
Sandoe JA, Kerr KG, Reynolds GW, et al. Staphylococcus capitis endocarditis: two cases and review of the literature. Heart 1999;82(3):e1.
Cui B, Smooker PM, Rouch DA, et al. Differences between Two Clinical Staphylococcus capitis Subspecies as Revealed by Biofilm, Antibiotic Resistance, and Pulsed-Field Gel Electrophoresis Profiling. J Clin Microbiol 2013;51(1):9-14.
Abera B, Kibret M, Mulu W. Knowledge and beliefs on antimicrobial resistance among physicians and nurses in hospitals in Amhara Region, Ethiopia. BMC Pharmacol Toxicol 2014;15(1):26.
Akhtar N. Staphylococcal Nasal Carriage of Health Care Workers. J Coll Physicians Surg Pak 2010;20(7):439-43.
Al-Abdli NE, Baiu SH. Nasal Carriage of Staphylococcus in Health Care Workers in Benghazi Hospitals. Am J Microbiol Res 2014;2(4):110-12.
Pourmand MR, Memarian M, Hoseini M, et al. High prevalence of sea gene among clinical isolates of Staphylococcus aureus in Tehran. Acta Med Iran 2009;47(5):357-61.
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