Prevalence of Hepatitis B and C among Patients Looking for Hospital Care; Five Years’ Study in Mashhad, Iran

  • Atefeh Yari Microbiology and Virology Research Center, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Hadis Yousefzadeh Student Research Committee, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Sahar Tahaghoghi Microbiology and Virology Research Center, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
  • Kiarash Ghazvini Mail Microbiology and Virology Research Center, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Hepatitis B virus, Hepatitis C virus, Real Time PCR, Viral load, Gender


This study was conducted to find out the prevalence, viral load and co-infection of HBV and HCV infection among patients seeking to hospital care in Mashhad, Iran. A total of 402 samples (349 samples for HBV and 53 for HCV) were received and were screened for hepatitis B and C during 2004 to 2014. Viral loads of HBV DNA and HCV RNA were quantified by real-time PCR. Among 349 collected samples, 229 (65.61%) were positive for HBV DNA and 36 (67.92%) for HCV RNA. Among the ones positive for HBV DNA and HCV RNA, HCV was more prevalent (86.11% Vs 58%) ,in male patients, a higher incidence was attributed to HBV than HCV (34.42 Vs 13.88%%). The incidence of co-infection of HBV and HCV was in 5 (1.88%) patients. Association of age and load of HBV, HCV showed that higher marginal viral loads found to be more common in the age groups of upper 30 years old (P=0.064, P=0.079, respectively). The present study provides the preliminary information about high HCV and HBV prevalence. Findings from the current study will be helpful for the better management and control of viral hepatitis among patients looking for hospital care.


Zhou HB, Hu JY, Hu HP. Hepatitis B virus infection and intrahepatic Cholangiocarcinoma. World J Gastroenterol 2014:20(19):5721-9.

Saffar H, Ajami A, Saffar MJ, et al. Prevalence of Hepatitis B Virus Seromarkers in Young Adults Vaccinated at Birth; Impact on the Epidemiology of Hepatitis B Infection in Iran. Hepat Mon 2014;14(5):e17263.

Al Baqlani SA, Sy BT, Ratsch BA, et al. MolecularEpidemiology and Genotyping of Hepatitis B Virus of HBsAg-Positive Patients in Oman. PLoS One 2014;(5):e97759.

Lin J, Wu JF, Zhang Q, et al. Virus-related liver cirrhosis: Molecular basis and therapeutic options. World J Gastroenterol 2014;20(21):6457-69.

World Health Organization. Hepatitis B vaccines. Wkly Epidemiol Rec 2009;48:405-19.

Zanetti AR, Van Damme P, Shouval D. The global impact of vaccination against hepatitis B: a historical overview. Vaccine 2008;26(49):6266-73.

Yan Z, Fan K, Wang Y, et al. Changing pattern of clinical epidemiology on hepatitis C virus infection in southwest china. Hepat Mon 2012;12(3):196-204.

Gacche RN, Kaid AM. Epidemiology of Viral Hepatitis B and C Infections in Ibb City, Yemen. Hepat Mon 2012;12(7):460-2.

Fathimoghaddam F, Hedayati-Moghaddam MR, BidkhorimHR, et al. The prevalence of hepatitis B antigen-positivity in the general population of Mashhad, Iran. Hepat Mon2011;11(5):346-50.

Liver Int Sievert W, Altraif I, Razavi HA, et al. A systematic review of hepatitis C virus epidemiology in Asia, Australia and Egypt. Liver Int 2011;31(Suppl2):S61-80.

Arababadi MK, Nasiri Ahmadabadi B, Yousefi Daredor H, et al. Epidemiology of occult hepatitis B infection among thalassemic, hemophilia, and hemodialysis patients. Hepat Mon 2012;12(5):315-19.

Babamahmoodi F, Heidari Gorji MA, Mahdi Nasehi M, et al. The prevalence rate of hepatitis B and hepatitis C coinfection in HIV positive patients in Mazandaran province, Iran. Med Glas (Zenica) 2012;9(2):299-303.

Kassaian N, Adibi P, Kafashaian A, Yaran M, et al. Hepatitis C virus and associated risk factors among prisoninmates with history of drug injection in Isfahan, Iran. Int J Prev Med 2012;3(Suppl 1):S156-61.

Zahedi MJ, Darvish Moghaddam S, Alavian SM, et al. Seroprevalence of hepatitis viruses B, C, D and HIV infection among hemodialysis patients in Kerman Province, South-East Iran. Hepat Mon 2012;12(5):339-43.

Noori S, Mohamadi A, Sarbazi MR, et al. Epidemiological features of hepatitis B and C infection in a high risk population: results of screening programs. Gastroenterol Hepatol Bed Bench 2013;6(3):136-140.

Ramezani A, Amirmoezi R, Volk JE, et al. HCV, HBV, and HIV seroprevalence, coinfections, and related behaviors among male injection drug users in Arak, Iran. AIDS Care 2014;26(9):1122-6.

Javadi A, Ataei B, Kassaian N, et al. Co-infection of human immunodeficiency virus, hepatitis C and hepatitis B virus among injection drug users in Drop in centers. J Res Med Sci 2014;19(Suppl 1):S17-21.

Foroughipour M, Jabbari Azad F, Farid Hosseini R, et al. Outcome of Intravenous Immunoglobulin-Transmitted HTLV-I, Hepatitis B, Hepatitis C, and HIV infections. Iran J Basic Med Sci 2013;16(3):221-4.

Amiri FB, Gouya MM, Saifi M, et al. Vulnerability of homeless people in Tehran, Iran, to HIV, tuberculosis and viral hepatitis. PLoS One 2014;9(6):e98742.

Raja A, Waheed K, Waqaruddin A, et al. Screening of HBsAg and anti HCV from tertiary care, private and public sector hospitals. Pak J Med Res 2011;50(1):20-3.

Khan J, Shafiq M, Mushtaq S, et al. Seropositivity and Coinfection of Hepatitis B and C among Patients Seeking Hospital Care in Islamabad, Pakistan. BioMed Res Int 2014;2014:516859.

Qureshi H, Bile KM, Jooma R, et al. Prevalence of hepatitis B and C viral infections in pakistan: findings of a national survey appealing for effective prevention and control measures. East Mediterr Health J 2010;16(Suppl):S15-23.

Gull A, Jamal N, Abbas H. Seropositivity of HbsAg and Anti HCV in Rawalpindi and Islamabad and analysis of risk factors. Ann Pak Inst Med Sci 2009;5(1):242-4.

Pennap GR, Yakubu A, Oyige O, et al. Prevalence ofhepatitis B and C virus infection among people of a local community in Keffi, Nigeria. Afr J Microbiol Res 2010;4(4):274-8.

Al-Ajlan A. Prevalence of hepatitis B and C among students of health colleges in Saudi Arabia, East Mediterr Health J 2011;17(10):759-62.

How to Cite
Yari A, Yousefzadeh H, Tahaghoghi S, Ghazvini K. Prevalence of Hepatitis B and C among Patients Looking for Hospital Care; Five Years’ Study in Mashhad, Iran. Acta Med Iran. 54(1):54-57.
Original Article(s)