Protective Effect of 25Mg-Porphyrin-Fullerene Nanoparticles on Oxygen-Glucose Deprivation/Reperfusion Injury in PC12 Cells

  • Peivand Ghasemzadeh Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
  • Seyed Mahdi Rezayat Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran. AND School of Advanced Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Sharareh Adeli Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  • Nahid Rahbar-Roshandel Mail Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Keywords:
25Mg magnetic isotope effect, 25MgPMC16 nanoparticles, ATP, Oxygen-glucose deprivation/reperfusion, PC12 cell line

Abstract

We investigated the effects of 25Mg-Porphyrin-Fullerene nanoparticles, (25MgPMC16) smart ferroporphyrin nanoparticles, on PC12 cells exposed to oxygen-glucose deprivation/reperfusion. In order to explore its effect on cells under oxygen-glucose deprivation conditions, the cultures were pretreated with 25MgPMC16 24 hours prior to oxygen-glucose deprivation/reperfusion. To initiate the oxygen-glucose deprivation/reperfusion, the cell culture medium was replaced with a glucose-free medium and the cells were transferred to a humidified incubation chamber in a mixture of 95% N2 and 5% CO2 at 37° C for 30, 60 and 120 min. Cell viability was assessed by MTT assay. Exposure of PC12 cells to 30, 60 and 120 min oxygen-glucose deprivation significantly decreased the cell viability. Pretreatment of the cultures with 25MgPMC16 significantly increased cell viability in a concentration-dependent manner. Pretreatment, the cultures with MK-801 (10 µM), a non-competitive NMDA antagonist, has attenuated the cell death after 30 min oxygen-glucose deprivation. We concluded that 25MgPMC16 could protect PC12 cells against oxygen-glucose deprivation/reperfusion-induced cell injury in a concentration-dependent manner. That could be due to the effect of 25MgPMC16 on ATP synthesis and the antioxidant effects of its components.

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Published
2016-09-17
How to Cite
1.
Ghasemzadeh P, Rezayat SM, Adeli S, Rahbar-Roshandel N. Protective Effect of 25Mg-Porphyrin-Fullerene Nanoparticles on Oxygen-Glucose Deprivation/Reperfusion Injury in PC12 Cells. Acta Med Iran. 54(8):478-484.
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