The Pattern of Methylation and Polymorphism in Interleukin-6 Promoter Gene Are Related to the Development of Rheumatoid Arthritis?

  • Isaura Isabelle Fonseca Gomes-Silva Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235-Cidade Universitária, Recife-PE, Brazil.
  • Eliezer Rushansky Department of Clinical Rheumatology, University of Pernambuco, Rua Arnóbio Marques, 10-Santo Amaro, Recife-PE, Brazil.
  • Francisco Geraldo Carvalho-Neto Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235-Cidade Universitária, Recife-PE, Brazil.
  • Amanda Virginia Batista Vieira Department of Biology, Rural Federal University of Pernambuco, Rua Manoel de Medeiros-Dois Irmãos, Recife-PE, Brazil.
  • Maria Helena Queiroz de Araujo Mariano Department of Clinical Rheumatology, University of Pernambuco, Rua Arnóbio Marques, 10-Santo Amaro, Recife-PE, Brazil.
  • Paulo Roberto Eleutério de Souza Department of Biology, Rural Federal University of Pernambuco, Rua Manoel de Medeiros-Dois Irmãos, Recife-PE, Brazil.
  • Martín Alejandro Montes Department of Biology, Rural Federal University of Pernambuco, Rua Manoel de Medeiros-Dois Irmãos, Recife-PE, Brazil.
  • Maria Mascena Diniz Maia Mail Department of Biology, Rural Federal University of Pernambuco, Rua Manoel de Medeiros-Dois Irmãos, Recife-PE, Brazil.
Keywords:
Cytokine, Inflammation, Epigenetics, MSP-PCR, Single nucleotide polymorphism

Abstract

Rheumatoid Arthritis (RA) is a complex disease with higher level of IL-6. In order to elucidate the alterations related to IL-6 gene in RA, we evaluated the -174 G/C IL-6 gene polymorphism and methylation pattern of its promoter. A total of 120 RA patients and 120 healthy controls were recruited for polymorphism analysis, and 30 individuals of both groups were randomly selected for methylation analysis. The IL-6 gene polymorphism was analyzed by PCR-RFLP and methylation pattern was analyzed by MSP-PCR. Regarding IL-6 gene polymorphism, no significant difference was found between RA patients and controls (P>0.05). For DNA, two CpG regions of IL-6 promoter gene was analyzed. The first region (−1069, −1061, −1057 and −1001 CpG) did not show significant differences, whereas the second region (−628, −610, −574 and −491 CpG) showed a significant hypermethylated status (P=0.0004) in RA patients as compared with controls.These results, suggest a possible relationship between methylation pattern and the susceptibility to RA in our studied population.

Author Biography

Maria Helena Queiroz de Araujo Mariano, Department of Clinical Rheumatology, University of Pernambuco, Rua Arnóbio Marques, 10-Santo Amaro, Recife-PE, Brazil.

 

 

 

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Published
2018-08-13
How to Cite
1.
Gomes-Silva IIF, Rushansky E, Geraldo Carvalho-Neto F, Virginia Batista Vieira A, Helena Queiroz de Araujo Mariano M, Roberto Eleutério de Souza P, Alejandro Montes M, Mascena Diniz Maia M. The Pattern of Methylation and Polymorphism in Interleukin-6 Promoter Gene Are Related to the Development of Rheumatoid Arthritis?. Acta Med Iran. 56(7):429-433.
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Articles