Frequency of KCNQ1 Variant rs2237892 in Type 2 Diabetes in East Azerbaijan Population, Northwest of Iran
,
Abstract
Genome-wide association studies in Asian population for the first time identified common variants in the KCNQ1 gene to be associated with type 2 diabetes in Japanese populations, recently. This result has been replied in some other East Asian population but, still now, there is no any report about this subject in Iranian population. The aim of this study was to investigate the frequency of KCNQ1 variants in type 2 diabetes in East Azerbaijan population, northwest of Iran. A total of 75 T2D and 90 normal East Azerbaijan subjects were entered the study. A demographic date was recorded for all subjects. Genomic DNA was extracted from the whole blood, and the KCNQ1 single nucleotide polymorphisms (SNPs) rs2237892 were genotyped with PCR-RFLP technique. The results of the study indicated an increased level of FBS and HbA1C among T2D cases (P<0/0001), as expected. Screening for KCNQ1 rs237892 among T2D case group revealed that the genotype frequencies of CC, CT and TT were 87.0% (67/77), 10.4% (8/77) and 2.6% (2/77), respectively. The allelic frequencies of C and T in case subjects were 92.21% (142/154) and 7.79% (12/154), respectively. Analysis of the normal control subjects showed the genotype frequencies of CC, CT and TT were 94.4% (85/90), 3.3% (3/90) 2/2% (2/90), respectively. The allelic frequencies of C and T among controls were 96.11% (173/180) and 3.89% (7/180), respectively. In conclusion, the results of our study indicated that there are no any significant differences in frequency of KCNQ1 rs237892 alleles between T2D and control subjects.
Federation I, Atlas ID. International Diabetes Federation. IDF diabetes atlas, 7th edn Brussels, Belgium: International Diabetes Federation. 2017.
Unoki H, Takahashi A, Kawaguchi T, Hara K, Horikoshi M, Andersen G, et al. SNPs in KCNQ1 are associated with susceptibility to type 2 diabetes in East Asian and European populations. Nat Genet 2008;40:1098-102.
Yasuda K, Miyake K, Horikawa Y, Hara K, Osawa H, Furuta H, et al. Variants in KCNQ1 are associated with susceptibility to type 2 diabetes mellitus. Nat Genet 2008;40:1092-7.
Neyroud N, Richard P, Vignier N, Donger C, Denjoy I, Demay L, et al. Genomic organization of the KCNQ1 K+ channel gene and identification of C-terminal mutations in the long-QT syndrome. Circ Res 1999;84:290-7.
Barhanin J, Lesage F, Guillemare E, Fink M, Lazdunski M, Romey G. KvLQT1 and IsK (minK) proteins associate to form the IKs cardiac potassium current. Nature 1996;384:78-80.
Towbin JA, Vatta M. Molecular biology and the prolonged QT syndromes. Am J Med 2001;110:385-98.
Thévenod F. Ion channels in secretory granules of the pancreas and their role in exocytosis and release of secretory proteins. Am J Physiol Cell Physiol 2002;283:C651-C72.
Lee Y, . Kang, SE,. Kim, SH,. Han, JS,. Kim, CH,. Kim, HJ,. Ahn, CW,. Cha, BS,. Nam, M,. Nam, CM,. and Lee, HC. Association between polymorphisms in SLC30A8, HHEX, CDKN2A/B, IGF2BP2, FTO, WFS1, CDKAL1, KCNQ1 and type 2 diabetes in the Korean population. J Hum Genet 2008;53:991-8.
Panaghie G, Abbott GW. The role of S4 charges in voltage-dependent and voltage-independent KCNQ1 potassium channel complexes. J Gen Physiol 2007;129:121-33.
Qi Q, Li H, Loos RJ, Liu C, Wu Y, Hu FB, et al. Common variants in KCNQ1 are associated with type 2 diabetes and impaired fasting glucose in a Chinese Han population. Hum Mol Genet 2009;18:3508-15.
Hu C, Wang C, Zhang R, Ma X, Wang J, Lu J, et al. Variations in KCNQ1 are associated with type 2 diabetes and beta cell function in a Chinese population. Diabetologia 2009;52:1322-5.
Saif-Ali R, . Muniandy, S,. Al-Hamodi, Z,. Lee, CH,. Ahmed, kh,. Al-Mekhlafi, AM,. and Shah Ismai, I. KCNQ1 Variants Associate with Type 2 Diabetes in Malaysian Malay Subjects. Ann Acad Med Singapore 2011;40:488-92.
Lee Y-H, Kang ES, Kim SH, Han SJ, Kim CH, Kim HJ, et al. Association between polymorphisms in SLC30A8, HHEX, CDKN2A/B, IGF2BP2, FTO, WFS1, CDKAL1, KCNQ1 and type 2 diabetes in the Korean population. J Hum Genet 2008;53:991-8.
Almawi WY, Nemr R, Keleshian SH, Echtay A, Saldanha FL, AlDoseri FA, et al. A replication study of 19 GWAS-validated type 2 diabetes at-risk variants in the Lebanese population. Diabetes Res Clin Pract 2013;102:117-22.
Müssig K, Staiger H, Machicao F, Kirchhoff K, Guthoff M, Schäfer SA, et al. Association of type 2 diabetes candidate polymorphisms in KCNQ1 with incretin and insulin secretion. Diabetes 2009;58:1715-20.
van Vliet-Ostaptchouk JV, van Haeften TW, Landman GW, Reiling E, Kleefstra N, Bilo HJ, et al. Correction: Common Variants in the Type 2 Diabetes KCNQ1 Gene Are Associated with Impairments in Insulin Secretion During Hyperglycaemic Glucose Clamp. PloS one 2012;7.
Bagherzadeh A, Nejati-Afkham A, Tajallizade-Khoob Y, Shafiee A, Sharifi F, Esfahani MA, et al. Association of cardiac autonomic neuropathy with arterial stiffness in type 2 diabetes mellitus patients. J Diabetes Metab Disord 2013;12:1.
Hong JH, Jang HW, Kang YE, Lee JH, Kim KS, Kim HJ, et al. Urinary chiro-and myo-inositol levels as a biological marker for type 2 diabetes mellitus. Dis Markers 2012;33:193-9.
Turki A, Mtiraoui N, Al-Busaidi AS, Khirallah M, Mahjoub T, Almawi WY. Lack of association between genetic polymorphisms within KCNQ1 locus and type 2 diabetes in Tunisian Arabs. Diabetes Res Clin Pract 2012;98:452-8.
Bazzi M, Nasr F, Alanazi M, Alamri A, Turjoman A, Moustafa A, et al. Association between FTO, MC4R, SLC30A8, and KCNQ1 gene variants and type 2 diabetes in Saudi population. Genet Mol Res 2014;13:10194-203.
Tan JT, Nurbaya S, Gardner D, Ye S, Tai ES, Ng DP. Genetic Variation in KCNQ1 Associates With Fasting Glucose andβ-Cell Function A Study of 3,734 Subjects Comprising Three Ethnicities Living in Singapore. Diabetes 2009;58:1445-9.
Tan JT, Ng DP, Nurbaya S, Ye S, Lim XL, Leong H, et al. Polymorphisms identified through genome-wide association studies and their associations with type 2 diabetes in Chinese, Malays, and Asian-Indians in Singapore. J Clin Endocrinol Metab 2010;95:390-7.
| Files | ||
| Issue | Vol 56, No 2 (2018) | |
| Section | Original Article(s) | |
| Keywords | ||
| Type 2 diabetes GWAS KCNQ1 rs237892 Polymorphism | ||
| Rights and permissions | |
|
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
